SV40/hTERT/Rb 不杤化系列商品 | 定量之高力價Lentivirus病毒液-ABM台灣代理

Abmgood不杤化系列商品immortalized reagenet

Abmgood公司於人類與老鼠不杤之細胞株上，具有幾十年的實驗經驗；提供一系列不杤 (immortalized)化之細胞株、及不杇化之試劑商品。ready-to-use定量好之retrovirus、lentiviru、 adenovirus，可直接加於細胞中進行感染表現；病毒液包括有SV40重組病毒、EBV重組病毒、hTERT病毒液、Myc基因病毒液、p53基因病毒液、Rb基因病毒液、Ras基因病毒液。提供106及109兩種包裝力價之病毒液；亦提供一系列病毒力價濃縮與定量試劑商品。

細胞不朽化的工具中，SV40 T antigen是最簡單且最常用來造成不朽化細胞生成的方法。多數的病毒基因和抑癌基因（p53、Rb等）的不活化相關，會誘導細胞處於replicative senescence的階段。目前也有研究顯示被感染的細胞，SV40 T anbtigen會誘導端粒酶（Telomerase）的活性。與端粒（telomere）長度相關的Telomerase Reverse Transcriptase protein (TERT)是一種不朽細胞的研究應用。平常細胞中，hTERT是處於不活化態，必要時會進行活化保持telomere的長度，避免細胞發生replicative senescence的現象。某些細胞hTERT 過度表現（over-expression）對於誘發細胞不朽化是無效的（如初代上皮細胞），反而會造成細胞死亡；同時表現hTERT catalytic subunit以及p53或RB siRNA在人類初代卵巢上皮細胞會造成細胞不朽化。過度表現Ras或Myc T58A mutants也有初代細胞不朽化的現象產生。

SV40 Cell Immortalization Reagents不杤化系列商品

Product	Vector Map (Click to View)	Description	Quantity	Cat. #
Adeno-SV40 Adenovirus	pAdeno-SV40	Adenovirus expressing SV40 large and small T antigens (10⁶ pfu/ml)	250 μl	G210
Lenti-SV40 Lentivirus	pLenti SV40	Lentivirus expressing SV40 whole gene (10⁶ IU/ml)	10ml	G203
Lenti-SV40 Lentivirus, High Titer	pLenti SV40	High Titer Lentivirus expressing SV40 whole gene (10⁹ IU/ml)	5 x 20 μl	LV612
Lenti-SV40T Lentivirus	pLenti-SV40-T	Lentivirus expressing SV40 large T antigen (10⁶IU/ml)	10ml	G256
Lenti-SV40T Lentivirus, High Titer	pLenti-SV40-T	High Titer Lentivirus expressing SV40 large T antigen (10⁹ IU/ml)	5 x 20 μl	LV613
Lenti-SV40Tt Lentivirus	pLenti-SV40-Tt	Lentivirus expressing SV40 large and small T antigens (10⁶ IU/ml)	10ml	G258
Lenti-SV40Tt Lentivirus, High Titer	pLenti-SV40-Tt	High Titer Lentivirus expressing SV40 large and small T antigens (10⁹ IU/ml)	5 x 20 μl	LV614
Retro-SV40 Retrovirus	pRetro-E2 SV40	Retrovirus expressing SV40 large and small T antigens (10⁶IU/ml)	10ml	G212

HPV Cell Immortalization Reagents不杤化系列商品

Lenti-HPV-16 E6/E7 Virus	pLenti-III-HPV-16 E6/E7	Lentivirus containing HPV-16 E6/E7 gene (10⁶ IU/ml)	10ml	G268
Lenti-HPV-16 E6/E7 Virus, High Titer	pLenti-III-HPV-16 E6/E7	High Titer Lentivirus containing HPV-16 E6/E7 gene (10⁹IU/ml)	5 x 20 μl	LV617

EBV Cell Immortalization Reagents不杤化系列商品

Product

Vector Map

(Click to View)

Description

Quantity

Cat. No.

EBV Virus

N/A

Virus containing EBV gene (10⁶ IU/ml)

10ml

G229

hTERT Cell Immortalization Reagents不杤化系列商品

Product	Vector Map (Click to View)	Description	Quantity	Cat. No.
Adeno-hTERT	pAdeno-hTERT	Adenovirus containing hTERT (10⁶ IU/ml)	250 μl	G205
Adeno-hTERT Antisense	pAdeno-hTERT Antisense	Adenovirus containing antisense hTERT (10⁶ IU/ml)	250 μl	G208
Lenti-CMV-hTERT-GFP-2A-Puro Virus	pLenti-CMV-hTERT-GFP-2A-Puro	Lentivirus containing hTERT with a GFP Reporter & resistance to Puromycin (10⁶ IU/ml)	10ml	LV623
Lenti-CMV-hTERT-GFP-2A-Puro Virus, High Titer	pLenti-CMV-hTERT-GFP-2A-Puro	High Titer Lentivirus containing hTERT with a GFP Reporter & resistance to Puromycin (10⁹ IU/ml)	5 x 20 μl	LV624
Lenti-CMV-hTERT-RFP-2A-Puro Virus	pLenti-CMV-hTERT-RFP-2A-Puro	Lentivirus containing hTERT with a RFP Reporter & resistance to Puromycin (10⁶ IU/ml)	10ml	LV625
Lenti-CMV-hTERT-RFP-2A-Puro Virus, High Titer	pLenti-CMV-hTERT-RFP-2A-Puro	High Titer Lentivirus containing hTERT with a RFP Reporter & resistance to Puromycin (10⁹ IU/ml)	5 x 20 μl	LV626
Lenti-EF1α-hTERT Virus	pLenti-EF1a-hTERT	Lentivirus containing hTERT (10⁶ IU/ml)	10ml	G706
Lenti-EF1α-hTERT Virus, High Titer	pLenti-EF1a-hTERT	High Titer Lentivirus containing hTERT (10⁹ IU/ml)	5 x 20 μl	LV616
Lenti-hTERT virus	pLenti-hTERT	Lentivirus containing hTERT (10⁶ IU/ml)	10ml	G200
Lenti-hTERT Virus, High Titer	pLenti-hTERT	High Titer Lentivirus containing hTERT (10⁹ IU/ml)	5 x 20 μl	LV615
Lenti-hTERT Antisense virus	pLenti-hTERT Antisense	Lentivirus containing antisense hTERT (10⁶ IU/ml)	10ml	G201
Lenti-hTERT-Neo Virus	pLenti-hTERT-Neo	Lentivirus containing hTERT with resistance to Neomycin (10⁶ IU/ml)	10ml	G204
Lenti-hTERT-Neo Virus, High Titer	pLenti-hTERT-Neo	High Titer Lentivirus containing hTERT with resistance to Neomycin (10⁹ IU/ml)	5 x 20 μl	LV622
Retro-E1/hTERT virus	pRetro-E1 hTERT	Retrovirus containing hTERT (10⁶ IU/ml)	10ml	G207

Gene Immortalization (Myc, p53, Rb, Ras, CDK4, BmiI) Reagents不杤化系列商品

Product	Vector Map (Click to View)	Description	Quantity	Cat. No.
Lenti-p53 siRNA Virus	Lenti-p53 siRNA	Recombinant p53 siRNA Lentivirus (10⁶ IU/ml)	10ml	G219
Lenti-p53 siRNA Virus, High Titer	Lenti-p53 siRNA	High Titer Recombinant p53 siRNA Lentivirus (10⁹ IU/ml)	5 x 20 μl	LV619
Lenti-Rb siRNA Virus	Lenti-Rb siRNA	Recombinant Rb siRNA Lentivirus (10⁶ IU/ml)	10ml	G223
Lenti-Rb siRNA Virus, High Titer	Lenti-Rb siRNA	High Titer Recombinant Rb siRNA Lentivirus (10⁹ IU/ml)	5 x 20 μl	LV621
Lenti-Bmi1 Virus, High Titer	pLenti-Bmi1	High Titer Recombinant Bmi1 Lentivirus (10⁹ IU/ml)	5 x 20 μl	LV608
Lenti-Bmi1 Virus	pLenti-Bmi1	Recombinant Bmi1 Lentivirus (10⁶ IU/ml)	10ml	LV610
Lenti-CDK4 Virus, High Titer	pLenti-CDK4	High Titer Recombinant CDK4 Lentivirus (10⁹ IU/ml)	5 x 20 μl	LV609
Lenti-CDK4 Virus	pLenti-CDK4	Recombinant CDK4 Lentivirus (10⁶ IU/ml)	10ml	LV611
Lenti-Myc T58A Virus	pLenti-Myc T58A	Recombinant Myc T58A Lentivirus (10⁶ IU/ml)	10ml	G217
Lenti-Myc T58A Virus, High Titer	pLenti-Myc T58A	High Titer Recombinant Myc T58A Lentivirus (10⁹ IU/ml)	5 x 20 μl	LV618
Lenti-Ras V12 Virus	pLenti-Ras V12	Recombinant Ras V12 Lentivirus (10⁶ IU/ml)	10ml	G221
Lenti-Ras V12 Virus, High Titer	pLenti-Ras V12	High Titer Recombinant Ras V12 Lentivirus (10⁹ IU/ml)	5 x 20 μl	LV620