【SARS-CoV-2 NP IgG/IgM檢測】冠狀病毒核蛋白IgG/IgM抗體（2019-nCoV-Ab-IgG/IgM)
人新型冠狀病毒核蛋白IgG抗體（2019-nCoV-Ab-IgG）ELISA試劑盒 |貨號AE64577HU
Human Novel Coronavirus Nucleoprotein IgG antibody (2019-nCoV-Ab-IgG) ELISA Kit
冠狀病毒(Coronaviruses)是具有正義 RNA 基因組(positive-sense RNA genome)和螺旋對稱核衣殼(nucleocapsid)的包膜病毒(enveloped viruses)。在病毒感染的原代細胞(virus-infected primary cells)和用表達 N 蛋白(N protein)的質粒轉染的細胞(cells transfected with plasmids)中，冠狀病毒核蛋白(Coronavirus nucleoproteins)定位於細胞質(cytoplasm)和核仁(nucleolus)，這是一種亞核結構(subnuclear structure)。冠狀病毒 N 蛋白(Coronavirus N protein) 是冠狀病毒 RNA 合成(coronavirus RNA synthesis)所必需的，具有可能參與模板轉換(template switch)的 RNA 伴侶活性(RNA chaperone activity)。核衣殼蛋白(Nucleocapsid protein)是冠狀病毒(coronavirus)中含量最豐富的蛋白質。在病毒粒子組裝(virion assembly)過程中，N 蛋白(N protein)與病毒 RNA (viral RNA)結合併導致形成螺旋狀核衣殼(helical nucleocapsid)。核衣殼蛋白(Nucleocapsid protein)是一種高度免疫原性的磷蛋白(phosphoprotein)，也與病毒基因組複製(genome replication)和調節細胞信號通路(cell signaling pathways)有關。由於N蛋白序列保守(conservation)，免疫原性強，因此選擇冠狀病毒N蛋白(N protein)作為診斷工具。
該試劑盒中提供的微量滴定板已預塗有 2019-nCoV (N) 蛋白。然後將樣品添加到帶有Horseradish Peroxidase (HRP) 綴合的anti-human IgG antibody 抗體的適當microtiter plate wells中並孵育。然後將底物溶液(substrate solutions)添加到每個孔中。只有那些含有 2019-nCoV-Ab-IgG 和酶聯anti-human IgG antibody 抗體的孔才會出現顏色變化。通過添加硫酸溶液(sulphuric acid solution)終止酶-底物反應，並在 450 nm ± 2 nm 波長下用分光光度計測量顏色變化。該方法對檢測人 2019-nCoV-Ab-IgG 具有高靈敏度(sensitivity)和出色的特異性(specificity)。未觀察到人 2019-nCoV-Ab-IgG 與類似物之間存在顯著的交叉反應(cross-reactivity)或干擾(interference)。
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
| Species Reactivity | Human (Homo sapiens) |
| UniProt | N/A |
| Abbreviation | 2019-nCoV-Ab-IgG |
| Alternative Names | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein |
| Range | Qualitative detection antibody |
| Sensitivity | Request Information |
| Sample Type | Serum |
| Detection Method | Indirect method |
| Analysis Method | Qualitative |
| Assay Duration | 1-3h |
| Sample Volume | 1-200 μL |
| Detection Wavelengt | 450 nm |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human 2019-nCoV-Ab-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample Type | 1：2 | 1：4 | 1：8 | 1：16 |
| Serum | 78-89% | 81-99% | 92-103% | 95-105% |
| EDTA plasma | 91-101% | 90-98% | 93-101% | 91-98% |
| Heparin plasma | 92-103% | 93-102% | 92-99% | 91-101% |
| Sample Type | Number | Recovery range (%) | Average(%) |
| Serum | 10 | 90-101 | 96 |
| EDTA plasma | 10 | 89-97 | 93 |
| Heparin plasma | 10 | 91-99 | 95 |
人新型冠狀病毒核蛋白IgG抗體（2019-nCoV-Ab-IgG）ELISA試劑盒 套組內容
| Reagents | Quantity | Reagents | Quantity |
| Assay plate (96 Wells) | 1 | Instruction manual | 1 |
| Standard (lyophilized) | 2 | Sample Diluent | 1 x 20 mL |
| Biotin-Conjugate (concentrate 100 x) | 1 x 120 μL | Biotin-Conjugate Diluent | 1 x 20 mL |
| Streptavidin-HRP (concentrate 100 x) | 1 x 120 μL | Streptavidin-HRP Diluent | 1 x 20 mL |
| Wash Buffer (concentrate 25 x) | 1 x 20 mL | Substrate Solution | 1 x 12 mL |
| Stop Solution | 1 x 10 mL | Adhesive Films | 4 |
人新型冠狀病毒核蛋白IgM抗體（2019-nCoV-Ab-IgM）ELISA試劑盒 | 貨號AE64578HU
Human Novel Coronavirus Nucleoprotein IgM antibody (2019-nCoV-Ab-IgM) ELISA Kit
冠狀病毒(Coronaviruses)是具有正義 RNA 基因組(positive-sense RNA genome)和螺旋對稱核衣殼(nucleocapsid)的包膜病毒(enveloped viruses)。在病毒感染的原代細胞(virus-infected primary cells)和用表達 N 蛋白(N protein)的質粒轉染的細胞(cells transfected with plasmids)中，冠狀病毒核蛋白(Coronavirus nucleoproteins)定位於細胞質(cytoplasm)和核仁(nucleolus)，這是一種亞核結構(subnuclear structure)。冠狀病毒 N 蛋白(Coronavirus N protein) 是冠狀病毒 RNA 合成(coronavirus RNA synthesis)所必需的，具有可能參與模板轉換(template switch)的 RNA 伴侶活性(RNA chaperone activity)。核衣殼蛋白(Nucleocapsid protein)是冠狀病毒(coronavirus)中含量最豐富的蛋白質。在病毒粒子組裝(virion assembly)過程中，N 蛋白(N protein)與病毒 RNA (viral RNA)結合併導致形成螺旋狀核衣殼(helical nucleocapsid)。核衣殼蛋白(Nucleocapsid protein)是一種高度免疫原性的磷蛋白(phosphoprotein)，也與病毒基因組複製(genome replication)和調節細胞信號通路(cell signaling pathways)有關。由於N蛋白序列保守(conservation)，免疫原性強，因此選擇冠狀病毒N蛋白(N protein)作為診斷工具。
該試劑盒中提供的微量滴定板已預塗有 2019-nCoV (N) 蛋白。然後將樣品添加到帶有Horseradish Peroxidase (HRP) 綴合的anti-human IgM antibody 抗體的適當microtiter plate wells中並孵育。然後將底物溶液(substrate solutions)添加到每個孔中。只有那些含有 2019-nCoV-Ab-IgM 和酶聯anti-human IgM antibody 抗體的孔才會出現顏色變化。通過添加硫酸溶液(sulphuric acid solution)終止酶-底物反應，並在 450 nm ± 2 nm 波長下用分光光度計測量顏色變化。該方法對檢測人 2019-nCoV-Ab-IgM 具有高靈敏度(sensitivity)和出色的特異性(specificity)。未觀察到人 2019-nCoV-Ab-IgM 與類似物之間存在顯著的交叉反應(cross-reactivity)或干擾(interference)。
| Species Reactivity | Human (Homo sapiens) |
| UniProt | N/A |
| Abbreviation | 2019-nCoV-Ab-IgM |
| Alternative Names | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein |
| Range | Qualitative detection antibody |
| Sensitivity | Request Information |
| Sample Type | Serum |
| Detection Method | Indirect method |
| Analysis Method | Qualitative |
| Assay Duration | 1-3h |
| Sample Volume | 1-200 μL |
| Detection Wavelengt | 450 nm |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
| Sample Type | 1：2 | 1：4 | 1：8 | 1：16 |
| Serum | 78-89% | 81-99% | 92-103% | 95-105% |
| EDTA plasma | 91-101% | 90-98% | 93-101% | 91-98% |
| Heparin plasma | 92-103% | 93-102% | 92-99% | 91-101% |
人新型冠狀病毒核蛋白IgG抗體（2019-nCoV-Ab-IgM）ELISA試劑盒 套組內容
| Reagents | Quantity | Reagents | Quantity |
| Assay plate (96 Wells) | 1 | Instruction manual | 1 |
| Standard (lyophilized) | 2 | Sample Diluent | 1 x 20 mL |
| Biotin-Conjugate (concentrate 100 x) | 1 x 120 μL | Biotin-Conjugate Diluent | 1 x 20 mL |
| Streptavidin-HRP (concentrate 100 x) | 1 x 120 μL | Streptavidin-HRP Diluent | 1 x 20 mL |
| Wash Buffer (concentrate 25 x) | 1 x 20 mL | Substrate Solution | 1 x 12 mL |
| Stop Solution | 1 x 10 mL | Adhesive Films | 4 |