人新型冠狀病毒核蛋白IgM抗體（2019-nCoV-Ab-IgM）ELISA試劑盒 | 貨號AE64578HU

Human Novel Coronavirus Nucleoprotein IgM antibody (2019-nCoV-Ab-IgM) ELISA Kit

冠狀病毒(Coronaviruses)是具有正義 RNA 基因組(positive-sense RNA genome)和螺旋對稱核衣殼(nucleocapsid)的包膜病毒(enveloped viruses)。在病毒感染的原代細胞(virus-infected primary cells)和用表達 N 蛋白(N protein)的質粒轉染的細胞(cells transfected with plasmids)中，冠狀病毒核蛋白(Coronavirus nucleoproteins)定位於細胞質(cytoplasm)和核仁(nucleolus)，這是一種亞核結構(subnuclear structure)。冠狀病毒 N 蛋白(Coronavirus N protein) 是冠狀病毒 RNA 合成(coronavirus RNA synthesis)所必需的，具有可能參與模板轉換(template switch)的 RNA 伴侶活性(RNA chaperone activity)。核衣殼蛋白(Nucleocapsid protein)是冠狀病毒(coronavirus)中含量最豐富的蛋白質。在病毒粒子組裝(virion assembly)過程中，N 蛋白(N protein)與病毒 RNA (viral RNA)結合併導致形成螺旋狀核衣殼(helical nucleocapsid)。核衣殼蛋白(Nucleocapsid protein)是一種高度免疫原性的磷蛋白(phosphoprotein)，也與病毒基因組複製(genome replication)和調節細胞信號通路(cell signaling pathways)有關。由於N蛋白序列保守(conservation)，免疫原性強，因此選擇冠狀病毒N蛋白(N protein)作為診斷工具。

該試劑盒中提供的微量滴定板已預塗有 2019-nCoV (N) 蛋白。然後將樣品添加到帶有Horseradish Peroxidase (HRP) 綴合的anti-human IgM antibody 抗體的適當microtiter plate wells中並孵育。然後將底物溶液(substrate solutions)添加到每個孔中。只有那些含有 2019-nCoV-Ab-IgM 和酶聯anti-human IgM antibody 抗體的孔才會出現顏色變化。通過添加硫酸溶液(sulphuric acid solution)終止酶-底物反應，並在 450 nm ± 2 nm 波長下用分光光度計測量顏色變化。該方法對檢測人 2019-nCoV-Ab-IgM 具有高靈敏度(sensitivity)和出色的特異性(specificity)。未觀察到人 2019-nCoV-Ab-IgM 與類似物之間存在顯著的交叉反應(cross-reactivity)或干擾(interference)。

Species Reactivity	Human (Homo sapiens)
UniProt	N/A
Abbreviation	2019-nCoV-Ab-IgM
Alternative Names	SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein
Range	Qualitative detection antibody
Sensitivity	Request Information
Sample Type	Serum
Detection Method	Indirect method
Analysis Method	Qualitative
Assay Duration	1-3h
Sample Volume	1-200 μL
Detection Wavelengt	450 nm

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%

Sample Type	1：2	1：4	1：8	1：16
Serum	78-89%	81-99%	92-103%	95-105%
EDTA plasma	91-101%	90-98%	93-101%	91-98%
Heparin plasma	92-103%	93-102%	92-99%	91-101%

人新型冠狀病毒核蛋白IgG抗體（2019-nCoV-Ab-IgM）ELISA試劑盒 套組內容

Reagents	Quantity	Reagents	Quantity
Assay plate (96 Wells)	1	Instruction manual	1
Standard (lyophilized)	2	Sample Diluent	1 x 20 mL
Biotin-Conjugate (concentrate 100 x)	1 x 120 μL	Biotin-Conjugate Diluent	1 x 20 mL
Streptavidin-HRP  (concentrate 100 x)	1 x 120 μL	Streptavidin-HRP Diluent	1 x 20 mL
Wash Buffer (concentrate 25 x)	1 x 20 mL	Substrate Solution	1 x 12 mL
Stop Solution	1 x 10 mL	Adhesive Films	4