Thunder-Link® Oligonucleotides 寡核酸標誌套組 貨號425-0000

Thunder-Link 抗體標幟套組 只需在oligonucleotide的3’或5’端設計-NH2修飾，輕鬆連接抗體和寡核苷酸。針對微量樣品使用，應用於免疫PCR（Immuno-PCR）。免疫PCR結合oligonucleotide於Immunoassay中，大幅提高偵測極限。內附control、separation column。Thunder-Link®PLUS能夠簡單快速地將抗體與寡核苷酸結合，具有高回收率的材料和卓越的清潔程序。 該試劑盒使用快速和簡單，克服了與標準結合方法相關的具耗時和冗長的方法。

Immuno-PCR應用

免疫PCR結合oligonucleotide於Immunoassay中，大幅提高偵測極限。在測試樣品例如全血和血清中，可精確和靈敏地檢測稀有細胞或低濃度的蛋白質。免疫PCR擴增，常規檢測單個核酸分子。與其模板雜交的DNA探針設計，提供高靈敏度和高特異性之方法。

INNOVA提供兩種商品，可將寡核苷酸連接到不同組的分子（例如抗體或金納米顆粒），讓免疫測定更多元化。Immuno-PCR靈敏度增加10至1000倍。亦可做為多重蛋白質診斷測定的平台。

Proximity ligation assay-Used to identify proteins co-located within cells.

Proximity extension assay-Variation on ImmunoPCR using two antibodies specific for different epitopes.

Immuno-PCR Data

Immuno-PCR data generated using Thunder-Link^®. A mouse monoclonal antibody specific for human CRP (clone C7) was purchased in unconjugated format from HyTest. The antibody was conjugated to an oligonucleotide using a Thunder-Link^® kit, and was used as a detection antibody in a sandwich immuno-PCR assay with a polyclonal anti-CRP antibody as the capture reagent.

The top graph shows a plot of the number of PCR cycles undertaken vs. fluorescence intensity generated by SYBR Green containing PCR probes at particular antigen concentrations. The bottom graph shows this data following conversion to antigen amount vs. cycle number to enable the calculation of a standard curve. The results show that the assay utilizes 1000-fold less capture antibody and 100-fold less detection antibody, and provides 1000-fold greater sensitivity than the equivalent ELISA.

Features	Benefits
Quick and easy to use	Save time, no specialist knowledge required
High levels of antibody and oligo recovery	Save precious reagents
Use your own oligo and antibody, at your desired ratio	Flexible
Freeze dried	Ships at ambient temperature, long shelf-life
Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
Unidirectional chemistry	No risk of cross-linking
Covalent bond	Highly stable conjugates
Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairs	Covers the majority of sequences
Linking chemistry works at both 5’ and 3’ end	Provides ability to combine with other modifications
Post-conjugation clean-up step	No interference from unbound oligo
Positive control antibody and oligo included	Enables confirmation of protocol success
Wide range of target proteins	Also applicable to antibody fragments and small proteins

產品編號	產品名稱	包裝
425-0000 425-0300	Thunder-Link® oligo conjugation system	1 reaction 3 reaction