【Nitrocefin 顯色頭孢菌素底物】chromogenic cephalosporin substrate CAS#41906-86-9

Nitrocefin | 顯色頭孢菌素底物(chromogenic cephalosporin substrate) CAS#41906-86-9 APExBIO貨號 B6052

產品介紹

Nitrocefin 是一種顯色頭孢菌素底物(chromogenic cephalosporin substrate)，用於檢測 β-內醯胺酶(β-lactamases)。 β-內醯胺酶(β-lactamases)是由各種微生物產生的酶，可介導對 β-內醯胺類抗生素(β-lactam antibiotics )，如青黴素(penicillins) 和頭黴素(cephamycins)的耐藥性。與其他檢測β-內醯胺酶( β-lactamases )的方法相比，頭孢硝唑( nitrocefin )更為靈敏，而且使用的材料少，設備便宜。可以通過測量 380 至 500 nm 區域之間的吸收變化來定量跟踪頭孢硝唑(nitrocefin)的顏色變化 [1]。

Nitrocefin is a chromogenic cephalosporin substrate which is used for detection of β-lactamases. β-lactamases are enzymes produced by various microbes that mediate resistance to β-lactam antibiotics such as penicillins and cephamycins. Comparing to other methods for β-lactamases detection, nitrocefin is more sensitive while using few materials and inexpensive equipment. The color change by nitrocefin can be followed quantitatively by measuring changes in absorption between 380 to 500 nm region[1].

Product Citation

1. Liu X, Ma L, et al. “Synergistic antibacterial mechanism of Bi(2)Te(3) nanoparticles combined with the ineffective β-lactam antibiotic cefotaxime against methicillin-resistant Staphylococcus aureus.” J Inorg Biochem. 2019 Jul;196:110687.” PMID:31004991

Chemical Properties

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	516.50
Cas No.	41906-86-9
Formula	C₂₁H₁₆N₄O₈S₂
Solubility	insoluble in EtOH; insoluble in H2O; ≥20.24 mg/mL in DMSO
Chemical Name	(6R,7R)-3-((E)-2,4-dinitrostyryl)-8-oxo-7-(2-(thiophen-2-yl)acetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
SDF	Download SDF
Canonical SMILES	[H][C@]1([C@@H]2NC(CC3=CC=CS3)=O)SCC(/C=C/C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O)=C(C(O)=O)N1C2=O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

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