H2DCFDA (DCFH-DA) ROS螢光探針 | Biotium 貨號10058

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)

H₂DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H₂DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein.H2DCFDA (DCFH-DA) 是一種細胞滲透性探針，用於檢測細胞內活性氧 (ROS)，可檢測細胞中的活性氧中間體。氧化時，H2DCFDA 變成發出高綠色螢光的 2′,7′-二氯螢光素。 (Ex/Em= 504/529 nm)

• λ_Ex/λ_Em (pH >6) = 504/529 nm (end product)
• White solid soluble in DMSO or DMF
• Store desiccated at -20°C and protect from light
• C₂₄H₁₆Cl₂O₇
• MW: 487.29
• [4091-99-0]

建議使用(依不同細胞需最佳化調整) 

1. H2DCFDA 溶於 DMSO 得到 10 mM 儲備液，使用前進一步稀釋。
2. 細胞與 5 µM H2DCFDA 溶液在 PBS 中於 37°C 避光孵育 30 分鐘，然後用 0.05% 胰蛋白酶-EDTA 溶液收集細胞，懸浮在新鮮培養基中，立即用流式細胞儀分析。
3. 如果需要，與 H2DCFDA 探針一起使用螢光素染料 DCFDA 的 ROS 不敏感修飾作為陽性對照。染色程序與 H2DCFDA 相同。

Biotium 提供範圍廣泛的螢光探針用於研究凋亡細胞(Apotosis)

The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.