麩醯胺酸/谷氨醯胺 (Glutamine, GLN)-Glutamine Colorimetric Assay Kit

麩醯胺酸/谷氨醯胺 (Glutamine, GLN) 是人體最豐富的游離氨基酸，是重要的細胞燃料，也是抗氧化劑穀胱甘肽 ( antioxidant glutathione , GSH) 的重要前體。麩醯胺酸/谷氨醯胺 (Glutamine, GLN) 在多種細胞特定過程中發揮調節作用，例如新陳代謝、蛋白質合成和降解以及呼吸爆發。嚴重的麩醯胺酸/谷氨醯胺 (Glutamine, GLN) 缺乏通常在危重疾病中迅速發生。谷氨醯胺 (Glutamine, GLN) 具有免疫和細胞調節的調節能力，麩醯胺酸/谷氨醯胺 (Glutamine, GLN) 可降低危重疾病的發病率和死亡率。熱休克蛋白 (heat shock proteins, HSP) 的表達對於應激或損傷時的細胞和組織保護至關重要。麩醯胺酸/谷氨醯胺 (Glutamine, GLN) 可通過熱休克蛋白 (heat shock proteins, HSP) 在疾病和損傷中充當代謝燃料和應激信號分子。麩醯胺酸/谷氨醯胺 (Glutamine, GLN) 具有增強損傷時熱休克蛋白 (heat shock proteins, HSP) 表達的能力，調節一些與代謝相關的基因的表達，信號轉導、細胞防禦和修復，並激活細胞內信號通路。

Glutamine 麩醯胺酸 (谷氨醯胺) 檢測分析試劑盒 | 貨號K556-100 (ABCAM ab197011)

Glutamine Colorimetric Assay Kit

產品描述：谷氨醯胺（Glutamine, Gln）是含有不帶電的醯胺作為側鏈的最豐富的氨基酸之一。 它通過谷氨酸(glutamate )和氨( ammonia)的縮合合成。 Gln被歸類為非必需氨基酸(non-essential amino acid)，然而，Gln在幾種生物過程中是重要的，例如蛋白質合成(Protein synthesis)，哺乳動物腎臟中 酸平衡(acid-balance )的調節和細胞生長。 它構成了細胞核苷酸(nucleotides )和己糖胺(hexosamines)合成的主要氮源。 富含谷氨醯胺的飲食有益患有克羅恩病(Crohn’s disease)，嚴重燒傷(severe burns)， HIV/AIDS艾滋病毒/艾滋病和癌症(cancer)的患者。 BioVision的谷氨醯胺比色分析試劑盒是一種簡單靈敏的分析方法，可檢測各種液體和組織中生物學相關的Glutamine濃度。 該測定基於谷氨醯胺水解為Glutamine，產生穩定的信號，其與樣品中的Glutamine的量成正比。 該測定可以在多種生物樣品中檢測少至25μM的Glutamine。

Cat # +Size	K556-100 (ABCAM ab197011)
Size	100 assays
Detection Method	Absorbance (450 nm)
Applications	Measurement of Glutamine in various biological samples
Features & Benefits	• Convenient & Rapid Protocol • This kit can detect as little as 25 µM of Glutamine in a variety of biological samples
Kit Components	• Hydrolysis Buffer • Development Buffer • Hydrolysis Enzyme Mix (Lyophilized) • Development Enzyme Mix (Lyophilized) • Developer (Lyophilized) • Gln Standard (Lyophilized)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

引用文獻

Leandro R. Soria, Hepatic glutamine synthetase augmentation enhances ammonia detoxification., J Inherit Metab Dis, Nov. 2019, 30724386

Mokgadi Violet Gwangwa, Effects of glutamine deprivation on oxidative stress and cell survival in breast cell lines. Biol Res, Mar 2019; 30917872.

Mus, F. et al., (2017) Diazotrophy overcomes the deleterious growth phenotype of glnE deletion in Azotobacter vinelandii, Appl. Environ. Microbiol, Oct.2017, online

Mus F et al., (2017) Diazotrophy overcomes the deleterious growth phenotype of glnE deletion in Azotobacter vinelandii, Appl. Environ. Microbiol., 2017, April 21: epub

Lim et al., Targeting Mitochondrial Oxidative Metabolism in Melanoma Causes Metabolic Compensation through Glucose and Glutamine Utilization. Cancer Res., Jul 2014; 74: 3535 – 3545.

Glutamine 麩醯胺酸 (谷氨醯胺) 檢測分析試劑盒 | 貨號abx298914

Glutamine 麩醯胺酸 (谷氨醯胺) 檢測分析試劑盒 Glutamine (Gln) Assay Kit可用於檢測血清、血漿、尿液、細胞裂解液、細胞培養基、組織提取物和其他生物液體中的谷氨醯胺（Glutamine, Gln）。The Glutamine (Gln) Assay Kit can be used to detect Glutamine (Gln) in Serum, plasma, urine, cell lysate, cell culture media, tissue extracts and other biological fluids.

Target	Glutamine (Gln)
Reactivity	General (All species)
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.
Validity	The validity for this kit is 6 months.
Detection Method	Colorimetric
Sample Type	Serum, plasma, urine, cell lysate, cell culture media, tissue extracts and other biological fluids.
CAS Number	56-85-9
Availability	Shipped within 7-12 working days.
Note	This product is for research use only.