Glutamine 麩醯胺酸 (谷氨醯胺) 檢測分析試劑盒 | 貨號K556-100 (ABCAM ab197011)

Glutamine Colorimetric Assay Kit

產品描述：谷氨醯胺（Glutamine, Gln）是含有不帶電的醯胺作為側鏈的最豐富的氨基酸之一。 它通過谷氨酸(glutamate )和氨( ammonia)的縮合合成。 Gln被歸類為非必需氨基酸(non-essential amino acid)，然而，Gln在幾種生物過程中是重要的，例如蛋白質合成(Protein synthesis)，哺乳動物腎臟中 酸平衡(acid-balance )的調節和細胞生長。 它構成了細胞核苷酸(nucleotides )和己糖胺(hexosamines)合成的主要氮源。 富含谷氨醯胺的飲食有益患有克羅恩病(Crohn’s disease)，嚴重燒傷(severe burns)， HIV/AIDS艾滋病毒/艾滋病和癌症(cancer)的患者。 BioVision的谷氨醯胺比色分析試劑盒是一種簡單靈敏的分析方法，可檢測各種液體和組織中生物學相關的Glutamine濃度。 該測定基於谷氨醯胺水解為Glutamine，產生穩定的信號，其與樣品中的Glutamine的量成正比。 該測定可以在多種生物樣品中檢測少至25μM的Glutamine。

Cat # +Size	K556-100 (ABCAM ab197011)
Size	100 assays
Detection Method	Absorbance (450 nm)
Applications	Measurement of Glutamine in various biological samples
Features & Benefits	• Convenient & Rapid Protocol • This kit can detect as little as 25 µM of Glutamine in a variety of biological samples
Kit Components	• Hydrolysis Buffer • Development Buffer • Hydrolysis Enzyme Mix (Lyophilized) • Development Enzyme Mix (Lyophilized) • Developer (Lyophilized) • Gln Standard (Lyophilized)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

引用文獻

Leandro R. Soria, Hepatic glutamine synthetase augmentation enhances ammonia detoxification., J Inherit Metab Dis, Nov. 2019, 30724386

Mokgadi Violet Gwangwa, Effects of glutamine deprivation on oxidative stress and cell survival in breast cell lines. Biol Res, Mar 2019; 30917872.

Mus, F. et al., (2017) Diazotrophy overcomes the deleterious growth phenotype of glnE deletion in Azotobacter vinelandii, Appl. Environ. Microbiol, Oct.2017, online

Mus F et al., (2017) Diazotrophy overcomes the deleterious growth phenotype of glnE deletion in Azotobacter vinelandii, Appl. Environ. Microbiol., 2017, April 21: epub

Lim et al., Targeting Mitochondrial Oxidative Metabolism in Melanoma Causes Metabolic Compensation through Glucose and Glutamine Utilization. Cancer Res., Jul 2014; 74: 3535 – 3545.