【Endosome胞內體分離和細胞分離試劑盒】 Invent Biotechnologies貨號ED-028
Endosome胞內體分離和細胞分離試劑盒 | Invent貨號ED-028
Endosome Isolation and Cell Fractionation Kit
Early endosomes早期胞內體主要來自彼此融合的初級內吞囊泡 (primary endocytic vesicles) ，為晚期胞內體late endosome maturation成熟之起點。除了在cell physiology正常細胞生理學中的作用外，endocytic processes內吞過程在諸如Alzheimer’s disease阿爾茨海默氏病和遺傳性溶酶體貯積病 (inherited lysosomal storage diseases) 等許多疾病中扮演關鍵作用。用於分離endosomes的傳統方法基於密度梯度超速離心 (density gradient ultracentrifugation)。該方案需要大量的起始材料，並且tedious繁瑣且time-consuming耗時。 Minute™ Endosome分離和細胞分離試劑盒 (Endosome Isolation and Cell Fractionation Kit, 貨號ED028) 提供基於spin-column-based旋轉柱的嶄新Endosome分離技術，該技術快速簡便，僅需要較少數量的培養細胞或mg毫克組織之檢體量。該試劑盒可以沉澱並顯著分離來自培養細胞或組織的早期的endosomes。
| Items | Quantity |
| Buffer A | 15 ml |
| Buffer B | 15 ml |
| Plastic Rods | 2 units |
| Filter Cartridge | 20 units |
| Tissue Dissociation Beads | 2.5 grams |
| Collection Tubes | 20 units |
ED-028引用文獻
- Xu et al., (2018) Phosphorylation-Mediated IFN-gR2 MembraneTranslocation Is Required to Activate Macrophage Innate Response. Cell 175, 1–16.
- Drelich, A., Judy, B., He, X., Chang, Q., Yu, S., Li, X., … & Ksiazek, T. (2018). Exchange Protein Directly Activated by cAMP Modulates Ebola Virus Uptake into Vascular Endothelial Cells. Viruses, 10(10), 563.
- Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.
- Thapa, N., Chen, M., Horn, H. T., Choi, S., Wen, T., & Anderson, R. A. (2020). Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4. Nature Cell Biology, 22(11), 1357-1370.
- Liang, X. H., Nichols, J. G., Hsu, C. W., & Crooke, S. T. (2021). Hsc70 Facilitates Mannose-6-Phosphate Receptor-Mediated Intracellular Trafficking and Enhances Endosomal Release of Phosphorothioate-Modified Antisense Oligonucleotides. nucleic acid therapeutics.
- Jin, J., Kim, C., Xia, Q., Gould, T. M., Cao, W., Zhang, H., … & Goronzy, J. J. (2021). Activation of mTORC1 at late endosomes misdirects T cell fate decision in older individuals. Science Immunology, 6(60).
- Gong, B., Guo, Y., Ding, S.et al.A Golgi-derived vesicle potentiates PtdIns4P to PtdIns3P conversion for endosome fission.Nat Cell Biol (2021). https://doi.org/10.1038/s41556-021-00704-y
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性；用於總蛋白提取，細胞分離和膜蛋白分離，只需簡單四步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。
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