凝膠蛋白質/核酸提取試劑盒 |Protein/Nucleic Acid Extraction Kit from Gels- Invent Biotechnologies貨號PN-019

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MinuteTM Protein/Nucleic Acid Extraction Kit from Gels
產品描述:INVENTBIOTECH提供一種快速且無儀器的蛋白質/核酸提取試劑盒。蛋白質/核酸可以高產率快速<10分鐘內以從凝膠中提取。(取決於凝膠染色的方法),洗脫緩衝液可以是含有洗滌劑的緩衝液或純水。洗脫體積在10到200μl之間 (洗脫的蛋白質不被明顯稀釋)。多個凝膠片可以在一個管中加工,最終的蛋白質濃度相對較高。傳統之Gel 提取蛋白質和/或核酸的最常用方法為Passive elution被動洗脫和electro elution電洗脫。是從polyacrylamide/agarose gels聚丙烯酰胺/瓊脂糖凝膠通過Passive elution被動洗脫進行蛋白質提取通常需要過夜培養,提取蛋白質和/或核酸。洗脫的蛋白質被明顯稀釋並需要進一步濃縮。通過電洗脫的蛋白質提取比被動洗脫更快(30分鐘至2小時),但它需要一種特殊的洗脫裝置,對於較大的蛋白質(> 70 Kda)效率較低。電緩衝液通常含有可能干擾下游應用的濃度的清潔劑和其他化學品。凝膠蛋白質/核酸提取試劑盒可應用於提取的蛋白質用於MALD-MS分析、動物免疫、蛋白質 – 蛋白質相互作用和蛋白質 – 核酸相互作用研究等。核酸可用於PCR、克隆和測序等。
the extraction efficiency is getting lower as molecular weight getting higher. The recommendation is to start with a gel piece containing high concentration of protein of interest and homogenize in a 0.2% SDS in water. Incubate at 4C overnight then pass though the column. This will give you better yield.Another way to increase yield is to use lower percentage of gel for protein separation.
Best Regards
Minute™蛋白萃取套組,使用創新『Column Filter』技術,效果更佳,完成只需 一 分 鐘
蛋白萃取只需要vortex、transfer、spin
- 簡單四步驟完成,相較同類型產品節省6-12個步驟,降低加藥產生的錯誤。
- 無須長時間等待,節省至少25分鐘!
- 免超音波震盪、避免蛋白加熱與變性。
- 免除重複性的wash步驟。
SD-001/SN-002 Minute™ Total Protein Extraction Kit
處理量:20 µl-500 µl
時間:1-8 min
Yield:2-8 mg/ml

和RIPA buffer相比,小分子蛋白可獲得較多保留【左】;在蛋白質萃取過程中可以維持蛋白質原有的磷酸化狀況【右】

和超音波震盪破碎相比,Minute蛋白萃取套組可保存較多3T3細胞的小分子蛋白,DNA binding protein HDAC1可完整保留不被去除
SC-003 Minute™ Cytoplasmic & Nuclear Extraction Kits
時間:<15 min
Yield:1-3 mg/ml
SM-005 Minute™ Plasma Membrane Protein Isolation Kit
處理量:1×106-50×106 cells/sample
時間:<45 min
Yield:1-3 mg/ml
MP-007 Minute™ Mitochondria Isolation Kit For Mammalian Cells And Tissues
處理量:5×106-50×106 cells/sample
時間:<30 min
Yield:1-3 mg/ml
NE-013 Minute™ Nuclear Envelope Protein Extraction Kit
處理量:10×106-20×106 cells/sample
時間:<45 min
Yield:10-50 µg/sample
HP-014 Minute™ Histone/DNA Binding Protein Extraction Kit
處理量:0.5×106-5×106 cells/sample
時間:<10 min
Yield:1-2.5 mg/ml
產品編號 | 產品名稱 | 包裝 |
SD-001/SN-002 | Minute™ Total Protein Extraction Kit for Animal Cultured Cells/Tissues | 50 Tests |
SC-003 | Minute™ Cytoplasmic and Nuclear Fractionation kit | 50 Tests |
SM-005 | Minute™ Plasma Membrane Protein Isolation Kit | 50 Tests |
MP-007 | Minute™ Mitochondrial Membrane Protein Isolation Kit | 50 Tests |
NE-013 | Minute™ Nuclaer Envelope Protein Extraction Kit | 50 Tests |
HP-014 | Minute™ Histon/DNA Binding Protein Extraction Kit | 50 Tests |
SN-006 | Minute™ Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues | 50 Tests |
SD-008/SN-009 | Minute™ Total Protein Extraction Kit for Plant Tissues | 50 Tests |
SN-010 | Minute™ Detergent-Free Plant Protein Extraction Kit | 50 Tests |
CP-011 | Minute™ Chloroplast Isolation Kit | 50 Tests |
SB-004 | Minute ™ Bacterial Total Protein Extraction Kit | 50 Tests |
SC-012 | Minute™ Single Cell Isolation Kit | 50 Tests |
YT-015 | Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls | 50 Tests |
YD-016 | Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls | 50 Tests |
YM-017 | Minute™ Yeast Mitochondria Enrichment Kit | 50 Tests |
MM-018 | Minute™ Plant Microsomal Membrane Extraction Kit | 50 Tests |
PN-019 | Minute™ Protein/Nucleic Acid Extraction Kit from Gels | 20 Tests |
PC-020 | Minute™ PCR Template Prep | 100 Tests |
HD-021 | Minute™ Protein Extraction Kit for Hair and Nails | 20 Tests |
AT-022 | Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes | 20 Tests |