Discovery: Detect between 7-274 targets per sample in over 10 different species.
Easy-to-use: Membranes with pre-bound antibody spots giving multiplex results in 24 hours using Western blotting equipment!
Sensitive: Detect up to 4 orders of magnitude and as little as 1pg/ml for some proteins.
【EGFR磷酸化抗體矩陣】Assaygenie Human EGFR Phosphorylation Array (SARB0062)
EGFR磷酸化抗體矩陣 | Assaygenie Human EGFR Phosphorylation Array (16 targets) (SARB0062)
Human EGFR Phosphorylation Array (16 targets) (SARB0062)
Assay Genie Antibody 膜陣列(Antibody Membrane Arrays)是多重陣列(multiplex arrays)，可測量樣品中 7 和 274 種蛋白質的相對水平，非常適合生物標誌物(biomarker)發現和驗證研究(validation studies)。半定量抗體膜陣列(semi-quantitative antibody membrane arrays)可用於 15 個以上疾病領域的 10 個物種。該技術使用夾心免疫測定原理，使用與蛋白質印跡相同的設備即可輕鬆讀取，因此無需昂貴的儀器。Assaygenie提供一系列抗體矩陣(Antibody Array )產品, 抗體矩陣(Antibody Array )基於 ELISA 的高通量平台，EGFR磷酸化抗體矩陣 | Assaygenie Human EGFR Phosphorylation Array (16 targets) (SARB0062)，同時檢測樣本內多個Biomarker的含量 (16種相關蛋⽩) AKT1, CREB1, GSK3A, GSK3B, HSPB1, MAP2K1, MAP2K3, MAP2K6, MAPK1, MAPK14|MAPK8, MTOR, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KB1, TP53。用於在細胞、組織、血清甚至培養基中進行有效的蛋白質表達譜分析。與蛋白質印跡法(Western Blots)或傳統 ELISA 等傳統方法相比，抗體陣列檢測的生產效率和成本效益要高得多。步驟簡單，使用方便，無需專用設備。
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Reproducible: Inter-array Coefficient of Variation (CV) is as low as 5%.
Validation: Validate your array hits using our PharmaGenie ELISA designed with the same antibody pairs as the membrane arrays.
Compatible: Antibody Arrays are compatible with any chemiluminescent imaging system.
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
| Product Sku: | SARB0062 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 16 |
| Gene Symbols: | EGFR, ERBB2, ERBB3, ERBB4 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | HER/ErbB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
| EGFR (P-Tyr845) | EGFR (P-Tyr992) | EGFR (P-Tyr1045) | EGFR (P-Tyr1068) | EGFR (P-Tyr1086) |
| EGFR (P-Tyr1148) | EGFR (P-Tyr1173) | EGFR (P-Ser1046/1047) | ErbB2 (P-Tyr877) | ErbB2 (P-Tyr1112) |
| ErbB2 (P-Tyr 1221/1222) | ErbB2 (P-Tyr1248) | ErbB2 (P-Thr686) | ErbB2 (P-Ser1113) | ErbB3 (P-Tyr1289) |
ErbB4 (P-Tyr1284)
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- Human EGFR Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set I
- Phosphatase Inhibitor Cocktail II
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates