【Cell Meter™吞噬作用檢測試劑】Phagocytosis Assay Kit, ATT Bioquest貨號21225
螢光吞噬作用檢測試劑盒-紅色螢光｜ATT Bioquest 貨號21225 (ATT Bioquest)
Cell Meter™ Fluorimetric Phagocytosis Assay Kit *Red Fluorescence*
吞噬作用(Phagocytosis)定義為細胞對質膜包膜(plasma-membrane envelope)內顆粒的細胞攝取(cellular uptake)。通過吞噬和破壞入侵的微生物，吞噬過程(phagocytosis)在先天免疫反應( innate immune response)中至關重要。通過清除凋亡小體維持組織穩態(tissue homeostasis)和重塑(remodeling )也需要吞噬作用。吞噬作用(phagocytosis)的攝取機制取決於顆粒的大小、受體-配體相互作用(receptor-ligand)以及細胞骨架(cytoskeleton)的參與。一旦內化(internalized)，吞噬體與溶酶體( lysosomes)融合形成次級吞噬溶酶體(secondary phagolysosome f)進行消化，導致 pH 值逐漸降低。Cell Meter™ 螢光吞噬作用檢測試劑盒(Cell Meter™ Fluorimetric Phagocytosis Assay Kit )採用獨特的 pH 依賴性 Protonex™ 600 紅色乳膠珠結合物。珠子處於隨時可用的懸浮液中。與大多數現有的螢光染料不同，Protonex™ 600 紅色-乳膠微珠結合物在細胞外是無螢光的。然而，當它們在酸性吞噬體(acidic phagosomes)/吞噬溶酶體(phagolysosomes)內部時，它的螢光會急劇增加。這一特性使其無需台盼藍淬滅步驟(trypan blue quenching step )即可輕鬆使用，是研究吞噬作用及其規律的強大工具。 Cell Meter™ Fluorimetric Phagocytosis Assay Kit 還包括一種綠色螢光細胞活力染料，可以通過螢光顯微鏡同時檢測活細胞和吞噬過程。該測定法也適用於螢光酶標儀( fluorescence micro-plate)和流式細胞術檢測( flow cytometry detections)。
Component A: Protonex 600 Red-Latex Beads Conjugate1 vial (15 µL)
Component B: CytoTrace™ Green1 vial
Component C: DMSO1 vial (100 µL)
Figure 1. Examination of phagocytosis in RAW 264.7 cells using Cell Meter™ Fluorimetric Phagocytosis Assay Kit (Cat# 21225). RAW 264.7 cells were incubated with (B) or without (A) Cytochalasin D for 30 min before Protonex™ 600 Red-Latex Beads in growth medium was added and incubated for 4 hours before Cell Tracker was added and incubated for 30 minutes. The images were taken using Keyence Fluorescence microscopy.
Authors: Ma, Wei and Ao, Shengxiang and Zhou, Jianping and Li, Jiaxin and Liang, Xin and Yang, Xue and Zhang, Hao and Liu, Boyang and Tang, Wanqi and Liu, Haoru and others,
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Journal: American Journal of Physiology-Cell Physiology (2019): C762–C775
Authors: Liu, Yihan and Liu, Zhujiang and Tang, Hao and Shen, Yicong and Gong, Ze and Xie, Nan and Zhang, Xu and Wang, Wengong and Kong, Wei and Zhou, Yuan and others, undefined
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Authors: Guentsch, Annemarie and Beneke, Angelika and Swain, Lija and Farhat, Katja and Nagarajan, Shunmugam and Wielockx, Ben and Raithatha, Kaamini and Dudek, Jan and Rehling, Peter and Zieseniss, Anke and others, undefined
Journal: Molecular and Cellular Biology (2016): MCB–00236
AAT Bioquest在全球擁有一支由經驗豐富的專業分銷商組成的強大網絡，為從小型研究機構到財富 500 強公司的各種客戶組合提供卓越的產品和定制服務。AAT Bioquest 5000 多種產品目錄包括 Fluo-8®、Cal-520®、Cell Meter™、Amplite™、CytoTrace™ 和 CytoTell™ 等產品線，這些品牌在生命科學研究界獲得了認可和讚譽。
Amine-Reactive Cell Meter™ Fixable Viability Dyes
Cell Meter™ fixable viability dyes are membrane-impermeant, amine-reactive dyes designed to discriminate live and dead cell populations in flow cytometry. These dyes readily penetrate dead cells with compromised membrane integrity and bind irreversibly to cellular amine proteins and other intracellular components producing significant cellular fluorescence. In live cells, these dyes cannot penetrate the membrane leaving only cell-surface amines to bind with and dimly fluoresce. The difference in intensity between viable and dead cells is significant, making it easy to exclude dead cells from analysis. Because staining is robust and stable, cells labeled with Cell Meter™ fixable viability dyes can be fixed, permeabilized, and probed for intracellular immunophenotyping. The Cell Meter™ fixable viability dyes are available in 9 fluorescent stains to provide users greater flexibility in multicolor panel design. Dyes included in this series are excited by primary excitation sources, including the 405, 488, 633, and 647 nm laser lines, and emit in common channels.
