吞噬試驗盒 (Red E. coli)｜EZCell™Phagocytosis Assay Kit 貨號: K964-100 (ABCAM ab235901)
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吞噬試驗盒｜EZCell™系列商品
商品: EZCell™吞噬試驗盒（Red E. coli）｜貨號: K964-100 (ABCAM ab235901)
哺乳動物吞噬作用是防止入侵病原體的重要一線防禦機制。連續清除死亡細胞，組織重塑以及獲取某些細胞的營養素也是必不可少的。吞噬作用是通過細胞表面受體識別和結合外來顆粒而引發的特殊形式的內吞作用，其吞噬和形成吞噬體。成熟的吞噬體轉化成通過酶和有毒過氧化物破壞病原體的吞噬體。大腸桿菌和其他細菌菌株通常用作吞噬試驗中的病原體。 BioVision的EZCell™吞噬測定試劑盒（Red E. coli）利用熱滅活的螢光預先標記的大腸桿菌顆粒作為快速準確檢測和定量螢光顯微鏡，分光光度計或流式細胞術的體外吞噬作用的工具。該試劑盒為激活劑和/或吞噬作用和Toll樣受體（TLR）配體抑制劑提供了強大的篩選系統。
Inhibition of phagocytosis. J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Panel A and B: images of nontreated cells. Panel C and D: treatment with Cytochalasin D.
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Blue line: untreated control cells; red line: macrophages with engulfed E. coli particles; green line: inhibition of phagocytosis by Cytochalasin D.
Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays. BioVision’s EZCell™ Phagocytosis Assay Kit (Red E. coli) utilizes heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors (TLR) ligands
| Size | 100 assays |
| SKU+Size | K964-100 |
| Detection Method | Fluorescence (Ex/Em = 540/570 nm) |
| Species Reactivity | Mammalian |
| Applications | Rapid detection, quantification and validation of phagocytosis in convenient 96-well format – Tracking ligand internalization and screening for effectors of phagocytosis |
| Features & Benefits | • Simple & Rapid Protocol • Convenient: Non-Radioactive, no special handling or disposal required • High-Throughput • Accurate: reproducible results with low intra & inter assay variability |
| Kit Components | • Phagocytosis Assay Buffer • Red E. Coli • 10X Quenching Solution |