【蛋白酶K-Proteinase K】標準等級/NGS等級/分子生物學等級-Abcam (Biovision)
【蛋白酶K-Proteinase K】標準等級/NGS等級/分子生物學等級-Abcam (Biovision)
蛋白酶K (Proteinase K)具有高活性以及穩定之結構，可進行低專一性降解。其屬於枯草蛋白酶類的絲氨酸蛋白酶 ( serine protease )，可被PMSF抑制。 BioVision提供不同等級之蛋白酶K (Proteinase K)。標準級 (Standard Grade, 貨號9250/貨號9251) ，NGS級 (NGS Grade, NGS等級重組蛋白酶K 貨號9247) 和分子生物學級 (Molecular Grade 分子等級重組蛋白酶K貨號9210/貨號9211) 之重組蛋白酶K (Proteinase K)。蛋白酶K(Proteinase K) 純度高，為高反應性絲氨酸蛋白酶 ( serine protease )，具消化天然蛋白質的能力(native proteins)，在足量酵素濃度及作用時間的環境下，蛋白質會完全被水解。可以失活酶( inactivating enzymes )，例如DNase和RNase，而無需進行變性過程(denaturation process)。 它在肽鍵處裂解( peptide bond )與脂族(aliphatic,)，芳族(aromatic )或疏水性氨基酸的羧酸基團(carboxylic acid group )相鄰。 與天然蛋白酶K(native Proteinase K)相比具有更高的活性，並且在室溫下更穩定。它可用於任何消化天然(native )和變性蛋白質(denatured proteins)的情況。蛋白酶K在65°C時最活躍。產品目錄請按此
Key Features:
• Enhanced Purity & Enzyme Activity
• RNase/DNase Free
• Stable and Active Over a Wide pH Range
• Cost Effective • Bulk Sizes Available
*註解: 蛋白酶K配製標準 (若商品為凍乾包裝)：20mg/ml蛋白酶K配製標準（proteinase K）：將200mg的蛋白酶K加入到9.5ml水中，輕輕搖動，直至蛋白酶K完全溶解。不要渦旋混合。加水定容到10ml，然後分裝成小份貯存於-20℃。
Applications:
• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines for amplification reactions
• Isolation of mRNA or genomic DNA from different tissues including mouse tail or from cultured cells
• For modifying glycoprotein for structural studies
• To treat tissue sections for in situ hybridization
• Improving cloning efficiency of PCR products
• For isolating bio-product to remove protein contaminants in industries such as leather, food, medicineintermediates, etc
Proteinase K, Recombinant, Molecular Grade, A serine protease which inactivates nucleases
產品描述：一種絲氨酸蛋白酶 (serine protease)，具有天然蛋白質的能力，從而在不依賴變性過程的情況下inactivating DNase和RNase等酶。 它在SDS和尿素存在下保持其活性。 它被二異丙基氟磷酸酯（diisopropyl fluorophosphates, DFP）和苯基甲磺酰氟（phenyl methane sulfonyl fluoride , PMSF）inactivated。
| Alternate Name | Protease K, Endopeptidase K, Tritirachium alkaline proteinase, Molecular Biology grade Proteinase K |
| Gene Symbol | PROK |
| Gene ID | 5621 |
| Accession # | P06873 |
| Source | Tritirachium album |
| Appearance | Lyophilized |
| Physical Form Description | Lyophilized with no additives |
| Molecular Weight | 28.9 kDa |
| Purity by SDS-PAGE | ≥90% |
| Reconstitution Instructions | In 50 mM Tris-HCl, 2 mM Calcium acetate, pH 8.0. |
| Handling | Centrifuge the vial prior to opening. |
NGS等級重組蛋白酶K 100 mg | Accession # P06873 貨號9247
Proteinase K, Recombinant, Molecular Grade, A serine protease which inactivates nucleases
產品描述：一種絲氨酸蛋白酶 (serine protease)，具有天然蛋白質的能力，從而在不依賴變性過程的情況下inactivating DNase和RNase等酶。 它在SDS和尿素存在下保持其活性。 它被二異丙基氟磷酸酯（diisopropyl fluorophosphates, DFP）和苯基甲磺酰氟（phenyl methane sulfonyl fluoride , PMSF）inactivated。
| Alternate Name | Protease K, Endopeptidase K, Tritirachium alkaline proteinase, NGS Grade Proteinase K, NGS Grade Protease K |
| Gene Symbol | PROK |
| Gene ID | 5621 |
| Accession # | P06873 |
| Source | Tritirachium album |
| Appearance | Lyophilized Powder |
| Physical Form Description | White lyophilized powder |
| Molecular Weight | 28.9 kDa |
| Purity by SDS-PAGE | Highly purified |
| Reconstitution Instructions | In water |
| Handling | Centrifuge the vial prior to opening. |
NGS等級重組蛋白酶K 100 mg | Accession # P06873 貨號9247
Proteinase K, Recombinant, Molecular Grade, A serine protease which inactivates nucleases
產品描述：一種絲氨酸蛋白酶 (serine protease)，具有天然蛋白質的能力，從而在不依賴變性過程的情況下inactivating DNase和RNase等酶。 它在SDS和尿素存在下保持其活性。 它被二異丙基氟磷酸酯（diisopropyl fluorophosphates, DFP）和苯基甲磺酰氟（phenyl methane sulfonyl fluoride , PMSF）inactivated。
| Alternate Name | Protease K, Endopeptidase K, Tritirachium alkaline proteinase, NGS Grade Proteinase K, NGS Grade Protease K |
| Gene Symbol | PROK |
| Gene ID | 5621 |
| Accession # | P06873 |
| Source | Tritirachium album |
| Appearance | Lyophilized Powder |
| Physical Form Description | White lyophilized powder |
| Molecular Weight | 28.9 kDa |
| Purity by SDS-PAGE | Highly purified |
| Reconstitution Instructions | In water |
| Handling | Centrifuge the vial prior to opening. |
| Product Name | Cat. No. | Size |
| 分生等級 (粉狀*)分子等級重組蛋白酶K 100 mg | 貨號9210 Molecular Grade | 9210 | 100 mg, 500 mg, 1 g, 10 g |
| 分生等級 (溶液) Proteinase K | 貨號9211-5 20 mg/ml Solution, Molecular Grade | 9211 | 5 ml, 25 ml, 100 ml |
| NGS等級 (粉狀*) NGS等級重組蛋白酶K 100 mg | 貨號9247 NGS Grade | 9247 | 100 mg, 500 mg, 1 g, 10 g |
| 標準款 (粉狀*) Proteinase K, Recombinant, Standard Grade | 9250 | 100 mg, 500 mg, 1 g, 10 g |
| 標準款 (溶液) Proteinase K, Recombinant ( 20 mg/ml Solution), Standard Grade | 9251 | 5 ml, 25 ml, 100 ml |
*註解: 蛋白酶K配製標準 (若商品為凍乾包裝)：20mg/ml蛋白酶K配製標準（proteinase K）：將200mg的蛋白酶K加入到9.5ml水中，輕輕搖動，直至蛋白酶K完全溶解。不要渦旋混合。加水定容到10ml，然後分裝成小份貯存於-20℃。