Abmgood不朽化系列商品 (immortalized cell)

根據細胞類型，Primary cells初代細胞在體外具有小於10-50次傳代的有限壽命。 不朽化細胞株能夠延長增殖，並且具有與親代組織相似或相同的基因型和表現型。哺乳動物細胞的不朽化Immortalized策略存在用於在培養物中不朽化哺乳動物細胞的幾種方法。 Abmgood多年研發經驗，開發出了一系列Immortalized商品；包括即用型逆轉錄病毒，慢病毒和腺病毒載體。

Gene Overexpressed	Viruses Offered	Application
hTERT	Adenovirus, Lentivirus, Retrovirus	可靠和常用。 可能在一些細胞類型（例如上皮細胞）中引起細胞死亡
SV40 T Antigen	Adenovirus, Lentivirus, Retrovirus	可靠和常用。 通常用於不朽化上皮細胞。
EBV genes	Epstein-Barr Virus	不朽化B淋巴細胞，有時是T淋巴細胞。
HPV E6-E7	Lentivirus	不朽化角化細胞。
HOXB8, HOXA9, HOXA10	Lentivirus	不朽化造血細胞，包括巨噬細胞，造血祖細胞和骨髓祖細胞。
Adenoviral E1A-E1B	Lentivirus	不朽化大範圍的初級囓齒動物細胞，包括羊水細胞，原發性上皮細胞，成纖維細胞和原代腎細胞。
p53, Rb siRNA	Lentivirus	與hTERT共表達以更好地使上皮細胞不朽化。
Bmi1	Lentivirus	不朽化上皮細胞。
CDK4	Lentivirus	與hTERT共同表達更真實的細胞模型。
Myc T58A (c-Myc), Ras V12 (H-Ras)	Lentivirus	不朽化各種細胞，包括神經幹細胞，初代腎細胞，成紅細胞和各種上皮細胞（原發性前列腺，乳腺，氣道等）。

HOT!! 黴漿菌PCR偵測 | DAPI染核 | 細胞內黴漿菌去除劑 | 水浴槽抑菌劑 | 噴霧式去黴劑 等系列商品

Abmgood不朽化系列商品 (immortalized reagenet) Ready-to-use

Abmgood公司於人類與老鼠不朽之細胞株上，具有幾十年的實驗經驗；提供一系列不朽 (immortalized)化之細胞株、及不朽化之試劑商品。ready-to-use定量好之retrovirus、lentiviru、 adenovirus，可直接加於細胞中進行感染表現；病毒液包括有SV40重組病毒、EBV重組病毒、hTERT病毒液、Myc基因病毒液、p53基因病毒液、Rb基因病毒液、Ras基因病毒液。提供106及109兩種包裝力價之病毒液；亦提供一系列病毒力價濃縮與定量試劑商品。

細胞不朽化的工具中，SV40 T antigen是最簡單且最常用來造成不朽化細胞生成的方法。多數的病毒基因和抑癌基因（p53、Rb等）的不活化相關，會誘導細胞處於replicative senescence的階段。目前也有研究顯示被感染的細胞，SV40 T anbtigen會誘導端粒酶（Telomerase）的活性。與端粒（telomere）長度相關的Telomerase Reverse Transcriptase protein (TERT)是一種不朽細胞的研究應用。平常細胞中，hTERT是處於不活化態，必要時會進行活化保持telomere的長度，避免細胞發生replicative senescence的現象。某些細胞hTERT 過度表現（over-expression）對於誘發細胞不朽化是無效的（如初代上皮細胞），反而會造成細胞死亡；同時表現hTERT catalytic subunit以及p53或RB siRNA在人類初代卵巢上皮細胞會造成細胞不朽化。過度表現Ras或Myc T58A mutants也有初代細胞不朽化的現象產生。

Catalog number	細胞培養商品
ABM-B561	Hemocytometer Cover Glass – 1 Slip 20mm x 20mm
ABM-TM032	IPL-41 Insect Medium – 1.0L
ABM-TM030	D-22 Insect Medium – 1.0L
ABM-TM035	Sheilds & Sang M3 Insect Medium – 1.0L
ABM-TM057	Sodium pyruvate solution (100mM) – 100ml
ABM-TM031	Grace’s Insect Medium – 1.0L
ABM-TM033	Mitsuhashi & Maramorosch Insect Medium – 1.0L
ABM-TM034	Schneider’s Insect Medium – 1.0L
ABM-TM036	TC-100 Insect Medium – 1.0L
ABM-TM037	TNM-FH Insect Medium – 1.0L
ABM-B560	Hemocytometer – 1 Plate 79mm x 39mm x 13mm
ABM-G255	Penicillin/Streptomycin Solution – 100ml
ABM-G273	Antibiotic/Antimycotic – 100ml
ABM-G275	L-glutamine – 100ml
ABM-G287	Luciferase Assay Kit – 500 Reactions
ABM-TM051	Trypsin-EDTA – 100ml
ABM-G299	PriCoat T25 Flasks – 10 Flasks
ABM-TM026	Serum Free Cryopreservation Medium – 25 ml
ABM-TM061	Poly-L-Lysine Coating Solution (0.1%) – 25ml
ABM-TM063	Gelatin Coating Solution (0.1%) – 100ml
ABM-G274	Amphotericin B (Fungizone) – 100ml
ABM-G299-T75	PriCoat T75 Flasks- Filter Cap – 5 Flasks
ABM-G271	Geneticin (Antibiotic G418) – 10ml
ABM-G272	Gentamicin – 10ml
ABM-B551	DAPI – 0.2ml
ABM-TM024	Cryopreservation Medium – 100ml
ABM-TM054	Bovine Serum Albumin Solution (7.5%) – 100ml
ABM-TM055	Apo-transferrin – 50mg
ABM-G599	Human Holo Transferrin – 100mg
ABM-TM053	Insulin Solution (10 mg/ml) – 5ml
ABM-TM062	Poly-L-Ornithine Coating Solution (0.1mg/ml) – 50ml
ABM-TM001	Prigrow I Medium – 500ml
ABM-TM002	Prigrow II Medium – 500ml
ABM-TM003	Prigrow III Medium – 500ml
ABM-TM004	Prigrow IV Medium – 500ml
ABM-B901	48-well Cell Culture Plates Flat-Bottom Individually Packed Sterile – 50 Plates
ABM-B902	24-well Cell Culture Plates Flat-Bottom Individually Packed Sterile – 50 Plates
ABM-B903	6-well Cell Culture Plates Flat-Bottom Individually Packed Sterile – 50 Plates
ABM-TM015	Prigrow V Medium – 500ml
ABM-TM016	Prigrow VI Medium – 500ml
ABM-TM017	Prigrow VII Medium – 500ml
ABM-TM018	Prigrow VIII Medium – 500ml
ABM-TM019	Prigrow IX Medium – 500ml
ABM-TM023	iPSC Freezing Media – 100ml
ABM-TM050	Trypsin-EDTA – 500ml
ABM-TM052	Insulin-Transferrin-Sodium Selenite Supplement (100X) – 10ml
ABM-TM020	Human iPSC Culture Medium Kit – 500ml
ABM-TM025	Neural Stem Cell Medium Kit – 100ml
ABM-B906	T225 Cell Culture Flasks Filter Cap Sterile – 5 Bags of 5
ABM-G238	PCR Mycoplasma Detection Kit – 100 Reactions
ABM-TM038	Recombinant human Serum Albumin – 100ug
ABM-G398	Mycoplasma Elimination Cocktail – 2 x 1.0ml
ABM-TM022	Mesenchymal Stem Cell (MSC) Medium Kit – 500ml
ABM-TM059	Fibronectin Coating Solution (1mg/ml) – 1mg
ABM-TM100	Prigrow X Medium – 500ml
ABM-B905	100mm Cell Culture Dishes TC Treated – 15 Bags of 20
ABM-B907	T175 Cell Culture Flasks Filter Cap Sterile – 8 Bags of 5
ABM-TM021	Rat iPS Cell Culture Medium Kit – 500ml
ABM-TM005	Adipocyte Differentiation Medium – 250ml
ABM-B900	96-well Cell Culture Plates Flat-Bottom Individually Packed Sterile – 100 Plates
ABM-B904	150mm Cell Culture Dishes TC Treated – 20 Bags of 5
ABM-B909	T25 Cell Culture Flasks Filter Cap Sterile – 20 Bags of 10
ABM-B914	T25 Cell Culture Flasks, plug seal cap, sterile – Case of 200
ABM-TM999-100	USDA Research Grade Origin Fetal Bovine Serum – 100ml
ABM-TM010	Prigrow X.1 Medium – 500ml
ABM-TM040	Keratinocyte Medium Kit – 1 Kit
ABM-TM041	Smooth Muscle Medium Kit – 1 Kit
ABM-TM042	Endothelial Medium Kit – 1 Kit
ABM-TM043	Mammary Epithelial Medium Kit – 1 Kit
ABM-TM044	Bronchial Epithelial Medium Kit – 1 Kit
ABM-TM045	Small Airway Epithelial Medium Kit – 1 Kit
ABM-TM046	Fibroblast Medium Kit – 1 Kit
ABM-TM047	Melanocyte Medium Kit – 1 Kit
ABM-TM048	Preadipocyte Medium Kit – 1 Kit
ABM-TM049	Skeletal Muscle Medium Kit – 1 Kit
ABM-TM060	Vitronectin Coating Solution (0.5mg/ml) – 0.2ml
ABM-TM072	Myocyte Medium Kit – 1 Kit
ABM-TM073	Adipocyte Medium Kit – 1 Kit
ABM-B908	T75 Cell Culture Flasks Filter Cap Sterile – 20 Bags of 5
ABM-G285	BacAway – 2 x 1.25ml
ABM-G422	Applied Cell Extracellular Matrix – 25ml
ABM-B913	Cell Factory, 10 layers, cell culture area 6335cm^2 – 1 factory
ABM-TM008	Dendritic Cell Differentiation Medium – 250ml
ABM-TM006	M1-Macrophage Differentiation Medium – 250ml
ABM-TM007	M2-Macrophage Differentiation Medium – 250ml
ABM-TM999-500	USDA Research Grade Origin Fetal Bovine Serum – 500ml