病毒核酸分離試劑盒（Column） | Virus Nucleic Acid Isolation Kit (Column Based)貨號PDC03-0100 

PureDireX – PDC03-0100

Note: Purification Kits / Extraction Kit / NA016-0100 / Virus DNA / Virus RNA / COVID-19 / SARS-CoV-2
Categories: Column Based

一、產品介紹

病毒核酸分離試劑盒(Virus Nucleic Acid Isolation Kit, 貨號PDC03-0100 ) 提供了20分鐘快速製備核酸樣本、操作簡單且經濟高效純化病毒DNA/ RNA方法；應用於無細胞樣品（如血清、血漿、體液和病毒感染細胞培養物的上清液）中分離病毒 DNA/RNA(viral DNA/RNA)。其獨特的緩衝系統可有效裂解細胞並降解蛋白質，使核酸易於與管柱(column)的玻璃纖維基質(glass fiber matrix )結合，去除可能干擾下游檢測的抑制劑，確保更高的檢測特異性、靈敏度和可重複性。污染物如鹽(salts)、代謝物(metabolites)和可溶性大分子細胞成分(soluble macromolecular)在洗滌步驟中被去除。無需酚抽提(phenol extraction)和乙醇沉澱(ethanol precipitation )步驟，高質量核酸在無RNase洗脫緩衝液中洗脫。適合直接測定的濃縮樣品（無需沉澱）。使用總核酸分離試劑盒（ Total Nucleic Acid Isolation Kit (Virus) ）分離的病毒 DNA/RNA 適用於各種常規應用: 包括Real-time PCR/RT-PCR、自動螢光 DNA 測序(Automated Fluorescent DNA Sequencing)、PCR 和其他酶促反應。僅需 20 分鐘即可製備核酸樣本。

二、產品說明

樣品體積每次反應：300 μl 的全血(whole blood)  、200 μl  病毒樣本、200 μl 血清(Serum)、200 μl 血漿(plasma)、200 μl 體液
套組提供形式：試劑及微型離心柱(mini spin column)
樣本材料：
操作時間：20分鐘
Elution volume：50 μl

三、病毒核酸分離試劑盒操作方法 (若有不同依原廠網站公告為主) 

Step 1 Lysis
1.Transfer up to 200 μl of the virus sample into a 1.5 ml microcentrifuge tube and add
400 μl of the Buffer V1. (If the sample is less than 200 μl, adjust the sample volume to
200 μl with the PBS)
2. Mix well and let it stand at the room temperature for 10 minutes.
Step 2 Nucleic Acid Binding
1.Add 450 µl of the Buffer V2 (ethanol added) to the sample lysate and shake vigorously.
2.Place a Column VN in a 2 ml Collection Tube.
3.Transfer 700 µl of the lysate mixture into the Column VN.
4.Centrifuge at 16,000 x g for 1 minute.
5.Discard the flow-through and place the Column VN back in the same Collection Tube.
6.Transfer the remaining lysate mixture to the Column VN.
7.Centrifuge at 16,000 x g for 1 minute.
8.Discard the flow-through and place the Column VN back in the same Collection Tube.
Step 3 Wash
1.Add 400 µl of the Buffer W1 into the Column VN.
2.Centrifuge at 16,000 x g for 30 seconds.
3.Discard the flow-through and place the Column VN back into the same Collection tube.
4.Add 600 µl of Buffer W2 (ethanol added) into the Column VN.
5.Centrifuge at 16,000 x g for 30 seconds.
6.Discard the flow-through and place the Column VN back into the same Collection tube.
7.Centrifuge at 16,000 x g again for 2 minutes to remove the residual Buffer W2.

Step 4 Elution
1. Place the Column VN in a clean 1.5 ml microcentrifuge tube (DNase and RNase free).
2.Add 50 μl Buffer RE or RNase-free water (pH is between 7.0 and 8.5) to the center
of each Column VN, let it stand for 2 minutes, and centrifuge at 14,000 x g for 2
minutes.

乾浴加熱水浴槽 |  Finnpipette F3 微量分注器 |  試管振盪器 Vortex Mixer  |  Genereach迷你型微量離心機  |   ELISA儀器

bio-helix.com

Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。

在改善生活質量和實現科學卓越的願望的驅使下，Bio-Helix以最高的誠信標準運營。還將這種精神延伸到我們的服務和全球價值中，以提供給世界各地的客戶。多樣化的員工隊伍和協作網絡形成了公司文化的完美結合。 Bio-Helix 不僅是一家機會均等的公司，而且還努力提供友好和成長的環境。

CAT#	NAME	Size
BP001CU	BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442)	Set
PMI09-0500	IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa)	500 μl
PMI11-0500	IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa)	500 μl
PMB01-0500	Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa)	500 μl
PMB10-0500	Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa)	500 μl
PMB11-0500	Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa)	500 μl
PMB12-0500	Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa)	500 μl
PMB13-0500	Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa)	500 μl
PMU12-0500	UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa)	500 μl
CCH321-B100ML	UltraScence Pico Plus Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
CCH345-B100ML	UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
CCH365-B100ML	UltraScence Femto Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
CCH375-B100ML	UltraScence Femto Plus Western Substrate (Patent No: US10,711,185)	100 ml (50ml+50ml)
PS002-B500ML	COOMASSIEnano Protein Staining Solution, 500ml	500 ml
PS003-B500ML	Ponceau S Protein Staining Solution	500 ml
BS001-B500ML	OneStep Blocker Western Blocking Solution and Signal Enhancer	500 ml
TM001-B200ML	TMB ELISA Substrate	100 ml (50ml+50ml)
DM001-R500	BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps)	500 μl
DM003-R500	BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps)	500 μl
DM012-R500	BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps)	500 μl
DM015-R500	BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps)	500 μl
DMF12-0100	OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye)	600 μl
DMF13-0100	OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye)	600 μl
LD011-1000	Prime Juice Preloading Fluorescent Stain	1 ml
LD003-0500	Novel Green Plus (20,000X) DNA Staining Reagent	500 μl
AGT002-0500	Agarose Tablets, 110 pcs	0.5g x 110
MB755-0100	Agarose Powder 100g -Molecular Biology Grade	100 g
MB755-0500	Agarose Powder 500g -Molecular Biology Grade	500 g
MB101-0500	Taq DNA Polymerase	500U/100μl
DP001-0100	nanoTaq™ Hot-Start DNA Polymerase	100 rxns 500U/100μl
DN001-0250	100 mM dNTP Set	4 x 250μl
DN0010	10 mM dNTP Mix	1 ml
MB200-P100	2X PCR SuperMix	100 rxns 2 x 1.25mL
MBA01-0100	OmniPCR Supermix w Fluorescent dye	100 rxns (2.5ml)
RK001-0050	RScript™ cDNA Synthesis Kit	50 rxns
RT001-0050	RScript™ Reverse Transcriptase 10,000U	50 rxns 10,000U/50μl
RT001-0250	RScript™ Reverse Transcriptase 50,000U	250 rxns 50,000U/250μl
RI001-0125	RIBOAssure™ RNase Inhibitor 5,000U	125 rxns 5,000U/125μl
QPR01-0100	PanProbes™ One-Step RT-qPCR Kit	100 reactions (20 μl vol)
QPD01-0100	PanProbes™ Universal qPCR MasterMix	100 reactions (20 μl vol)
QSR01-0100	PanGreen™ One-Step RT-qPCR Kit	100 reactions (20 μl vol)
QSD01-0100	PanGreen™ Universal SYBR® Green Mastermix	100 reactions (20 μl vol)
PDP01-0100	Plasmid miniPREP Kit	100 rxns
PDP02-0020	Plasmid midiPREP Kit	20 rxns
PDP03-0010	Plasmid maxiPREP Kit	10 rxns
PDC01-0100	PCR Clean-Up & Gel Extraction Kit	100 rxns
PDC02-0100	Genomic DNA isolation dual kit	100 rxns
PDC05-0100	Dual Genomic DNA Isolation Kit (Plant)	100 rxns
PDC06-0100	Dual Genomic DNA Isolation Kit(Tissue)	100 rxns
PDC09-0100	Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)	100 rxns
PDC10-0100	Genomic DNA Isolation Kit (Plant)	100 rxns
PDC11-0100	Genomic DNA Isolation Kit (Tissue)	100 rxns
PDC12-0100	Genomic DNA Isolation Kit (Paraffin-embedded tissue)	100 rxns
PDM01-0100	Mbead Buffy Coat Genomic DNA Kit	100 rxns
PDM02-0100	Mbead Tissue Genomic DNA Kit	100 rxns
PDM03-0100	Mbead Bacteria Genomic DNA Kit	100 rxns
PDM05-0100	Mbead Plant Genomic DNA Kit	100 rxns
PDM06-0100	Mbead Blood/Cell Genomic DNA Kit	100 rxns
PDR01-0100	Genomic DNA Isolation Reagent	100 rxns
PDR02-0100	Plant Genomic DNA Isolation Reagent	100 rxns
PDR05-0100	Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue)	100 rxns
PDC04-0100	Total RNA Isolation Kit (Blood/Cultured Cell/Fungus)	100 rxns
PDC07-0100	Total RNA Isolation Kit (Plant)	100 rxns
PDC08-0100	Total RNA Isolation Kit(Tissue)	100 rxns
PDR03-0100	Total RNA Isolation Reagent	100 rxns
PDR04-0100	Plant Total RNA Isolation Reagent	100 rxns
PDC03-0100	Virus Nucleic Acid Isolation Kit	100 rxns
PDM04-0100	Mbead Virus Genomic Nucleic Acid Kit	100 rxns
QP019M-0050	COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa)	50 rxns
QP019T-0100	COVID-19 RT-qPCR Detection Kit Plus (TFDA)	100 rxns
QP019-0100	SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO)	100 rxns