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蛋白質電泳標準液 | 蛋白質分子量標準液 Blu12 (BLUeye) Prestained Protein Ladder(11 to 245 kDa)貨號PM007-0500/PMB12-0500

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蛋白質電泳標準液 | 蛋白質分子量標準液 Blu12 (BLUeye) Prestained Protein Ladder(11 to 245 kDa)貨號PM007-0500  (Genedirex) 

PMB12-0500 / PM007-0500

一、產品介紹

預染型蛋白質分子量標準液(Prestained Protein Ladder) Blu12 (BLUeye) Prestained Protein Ladder 在 SDS-聚丙烯酰胺凝膠電泳期間跟踪蛋白質的大小和分離,估計蛋白質大小並驗證 PVDF、尼龍或硝酸纖維素膜上的蛋白質轉移效率。(分子量11kDa、17kDa、20kDa、25kDa、35kDa、48kDa、63kDa、75kDa、100kDa、135kDa、180kDa、245kDa)

操作手冊

二、產品說明

Blu12 (BLUeye) Prestained Protein Ladder是一種即用型預染分子量標準液,擁有三種不同顏色的預染型蛋白標記 (其中 1 條 25 kDa 的綠色條帶和 75 kDa 的紅色條帶作為參考條帶) ,有12種不同分子量蛋白標示,分子量範圍從11 到 245 kDa,使用方便,無須任何前處理即可使用。

三、產品優點

  1. 紅綠藍三種顏色(25 kDa 的綠色條帶和 75 kDa 的紅色條帶作為參考條帶)
    2. 分子量範圍從11 到 245 kDa
    3. -20℃保存可長達兩年

The Blu12 (BLUeye) Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 245 kDa. The 12 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu12 (BLUeye) Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

 

Storage Buffer : 
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.

 

Storage : 
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

 

CAT# NAME Size
BP001CU BluPAD, LED Transilluminator, with mini Darkroom
(CE/ETL, Patent No: M543442)
Set
PMI09-0500 IRIS9 Plus Prestained Protein Ladder
(9 pre-stained bands, 15-170 kDa)
500 μl
PMI11-0500 IRIS11 Prestained Protein Ladder
(11 pre-stained bands, 3-260 kDa)
500 μl
PMB01-0500 Blu10 Plus Prestained Protein Ladder
(10 pre-stained bands, 6.5-270 kDa)
500 μl
PMB10-0500 Blu10 Prestained Protein Ladder
(10 pre-stained bands, 11-180 kDa)
500 μl
PMB11-0500 Blu11 Prestained Protein Ladder
(11 pre-stained bands, 10-180 kDa)
500 μl
PMB12-0500 Blu12 Prestained Protein Ladder
(12 pre-stained bands, 11-245 kDa)
500 μl
PMB13-0500 Blu13 Prestained Protein Ladder
(13 pre-stained bands, 5-245 kDa)
500 μl
PMU12-0500 UNveil Unstained Protein Ladder, Broad Range
(12 unstained bands, 10-200 kDa)
500 μl
CCH321-B100ML UltraScence Pico Plus Western Substrate
(Patent No: US10,711,185)
100 ml
(50ml+50ml)
CCH345-B100ML UltraScence Pico Ultra Western Substrate
(Patent No: US10,711,185)
100 ml
(50ml+50ml)
CCH365-B100ML UltraScence Femto Western Substrate
(Patent No: US10,711,185)
100 ml
(50ml+50ml)
CCH375-B100ML UltraScence Femto Plus Western Substrate
(Patent No: US10,711,185)
100 ml
(50ml+50ml)
PS002-B500ML COOMASSIEnano Protein Staining Solution, 500ml 500 ml
PS003-B500ML Ponceau S Protein Staining Solution 500 ml
BS001-B500ML OneStep Blocker
Western Blocking Solution and Signal Enhancer
500 ml
TM001-B200ML TMB ELISA Substrate 100 ml
(50ml+50ml)
DM001-R500 BH 100bp DNA Ladder RTU
(11 bands, 100-1,500bps)
500 μl
DM003-R500 BH 100bp DNA Ladder H3 RTU
(12 bands, 100-3,000bps)
500 μl
DM012-R500 BH 50bp DNA Ladder RTU
(17 bands, 50-1,500bps)
500 μl
DM015-R500 BH 1Kb Plus DNA Ladder RTU
(13 bands, 100-10,000bps)
500 μl
DMF12-0100 OmniMARK 100 DNA Ladder RTU
(12 bands, 100-3,000bps, fluorescent dye)
600 μl
DMF13-0100 OmniMARK 1K DNA Ladder RTU
(13 bands, 100-10,000bps, fluorescent dye)
600 μl
LD011-1000 Prime Juice Preloading Fluorescent Stain 1 ml
LD003-0500 Novel Green Plus (20,000X) DNA Staining Reagent 500 μl
AGT002-0500 Agarose Tablets, 110 pcs 0.5g x 110
MB755-0100 Agarose Powder 100g -Molecular Biology Grade 100 g
MB755-0500 Agarose Powder 500g -Molecular Biology Grade 500 g
MB101-0500 Taq DNA Polymerase 500U/100μl
DP001-0100 nanoTaq™ Hot-Start DNA Polymerase 100 rxns
500U/100μl
DN001-0250 100 mM dNTP Set 4 x 250μl
DN0010 10 mM dNTP Mix 1 ml
MB200-P100 2X PCR SuperMix 100 rxns
2 x 1.25mL
MBA01-0100 OmniPCR Supermix w Fluorescent dye 100 rxns
(2.5ml)
RK001-0050 RScript™ cDNA Synthesis Kit 50 rxns
RT001-0050 RScript™ Reverse Transcriptase 10,000U 50 rxns
10,000U/50μl
RT001-0250 RScript™ Reverse Transcriptase 50,000U 250 rxns
50,000U/250μl
RI001-0125 RIBOAssure™ RNase Inhibitor 5,000U 125 rxns
5,000U/125μl
QPR01-0100 PanProbes™ One-Step RT-qPCR Kit 100 reactions
(20 μl vol)
QPD01-0100 PanProbes™ Universal qPCR MasterMix 100 reactions
(20 μl vol)
QSR01-0100 PanGreen™ One-Step RT-qPCR Kit 100 reactions
(20 μl vol)
QSD01-0100 PanGreen™ Universal SYBR® Green Mastermix 100 reactions
(20 μl vol)
PDP01-0100 Plasmid miniPREP Kit 100 rxns
PDP02-0020 Plasmid midiPREP Kit 20 rxns
PDP03-0010 Plasmid maxiPREP Kit 10 rxns
PDC01-0100 PCR Clean-Up & Gel Extraction Kit 100 rxns
PDC02-0100 Genomic DNA isolation dual kit 100 rxns
PDC05-0100 Dual Genomic DNA Isolation Kit (Plant) 100 rxns
PDC06-0100 Dual Genomic DNA Isolation Kit(Tissue) 100 rxns
PDC09-0100 Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) 100 rxns
PDC10-0100 Genomic DNA Isolation Kit (Plant) 100 rxns
PDC11-0100 Genomic DNA Isolation Kit (Tissue) 100 rxns
PDC12-0100 Genomic DNA Isolation Kit (Paraffin-embedded tissue) 100 rxns
PDM01-0100 Mbead Buffy Coat Genomic DNA Kit 100 rxns
PDM02-0100 Mbead Tissue Genomic DNA Kit 100 rxns
PDM03-0100 Mbead Bacteria Genomic DNA Kit 100 rxns
PDM05-0100 Mbead Plant Genomic DNA Kit 100 rxns
PDM06-0100 Mbead Blood/Cell Genomic DNA Kit 100 rxns
PDR01-0100 Genomic DNA Isolation Reagent 100 rxns
PDR02-0100 Plant Genomic DNA Isolation Reagent 100 rxns
PDR05-0100 Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue) 100 rxns
PDC04-0100 Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) 100 rxns
PDC07-0100 Total RNA Isolation Kit (Plant) 100 rxns
PDC08-0100 Total RNA Isolation Kit(Tissue) 100 rxns
PDR03-0100 Total RNA Isolation Reagent 100 rxns
PDR04-0100 Plant Total RNA Isolation Reagent 100 rxns
PDC03-0100 Virus Nucleic Acid Isolation Kit 100 rxns
PDM04-0100 Mbead Virus Genomic Nucleic Acid Kit 100 rxns
QP019M-0050 COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa) 50 rxns
QP019T-0100 COVID-19 RT-qPCR Detection Kit Plus (TFDA) 100 rxns
QP019-0100 SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO) 100 rxns

 

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