咖啡因(Caffeine)檢測-Caffeine ELISA Kit

咖啡因(Caffeine) 是一種天然的精神活性物質，屬於一組稱為嘌呤生物鹼(purine alkaloids)的化合物。咖啡因(Caffeine)存在於多種植物中，如咖啡(coffee) 、茶(tea)、可可(cocoa)、瓜拉那(guarana) 和馬黛茶(yerba mate)。它經常被添加到膳食補充劑中，因為它能夠促進新陳代謝並有助於減肥。咖啡因是西方國家使用最廣泛的精神興奮劑，具有抗氧化(antioxidant)、抗發炎(anti-inflammatory)和抗細胞凋亡(anti-apoptotic)的特性。對於阿茲海默症 (Alzheimer’s disease, AD)，咖啡因對男性和女性、人類和動物都有益處。在患有帕金森氏症 (Parkinson’s disease, PD) 的男性中也觀察到了咖啡因的類似作用。

咖啡因檢測試驗盒Caffeine ELISA 試劑盒 -ELK Biotechnology貨號ELK0683

Caffeine ELISA Kit

靈敏度156.25-10000 pg/mL、最低靈敏度49.5 pg/mL

Sample Type:Serum, plasma, tissue homogenates and other biological fluids

產品介紹:

咖啡因(Caffeine)檢測試驗盒 | 貨號ELK0683 (Competitive Inhibition)，偵測的靈敏度0.47-30 ng/mL、最低靈敏度0.24 ng/mL。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對咖啡因(Caffeine)具有高靈敏度和出色的特異性，未觀察到咖啡因(Caffeine)與類似物之間存在明顯的交叉反應或干擾。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA。可應用於serum, plasma, tissue homogenates, cell lysates, cell culture。並在 450nm ± 10nm 的波長下用分光光度計測量顏色變化。然後通過將樣品的 OD 與標準曲線進行比較來確定樣品中咖啡因(Caffeine)的濃度。

Product name:	Caffeine ELISA Kit
Reactivity:	General
Alternative Names:	Caffeine
Assay Type:	Competitive Inhibition
Sensitivity:	0.24 ng/mL
Standard:	30 ng/mL
Range:	0.47-30 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	2.5h
Research Area:	Signal transduction;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Caffeine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caffeine. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of Caffeine in the samples is then determined by comparing the OD of the samples to the standard curve.

ELK原廠網址  >> http://www.elkbiotech.com/ 

ELK Biotechnology公司是一家專注於生命科學、免疫研究的高科技生物企業，位於Biolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology是一家專注於免疫產品和分子生物學產品研發和生產的高科技生物公司。產品涵蓋ELISA試劑盒、抗體、基因組提取試劑、分子生物學試劑。涉及免疫化學、腫瘤研究、神經生物學、細胞週期、信號轉導等領域。我們的產品有嚴格的研發和質量檢測流程。每個產品都有完整的研發、生產和質檢記錄。經驗豐富的研發人員，完善的質檢支持部門，嚴格的檢測流程，為客戶提供質量可靠的科研產品。

​​​