澱粉樣β胜肽1-40 (Ab1-40) ELISA試劑盒 | USCN貨號UCEA864Hu
澱粉樣β胜肽1-40 (Ab1-40) ELISA試劑盒 | USCN貨號UCEA864Hu
ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40) 12.35-1,000pg/mL
商品: 澱粉樣β胜肽1-40 (Ab1-40) ELISA試劑盒 (酶聯免疫吸附試驗法) Competitive Inhibition貨號UCEA864Hu，應用物種Homo sapiens (Human) ；偵測範圍12.35-1,000pg/mL, 最低靈敏度5.95pg/mL。可應用於serum, plasma and other biological fluids生物樣品之檢測。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。USCN提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。此產品需由儀器進行定量 ELISA儀器設備請按此
乾浴加熱水浴槽 | Finnpipette F3 微量分注器 | 試管振盪器 Vortex Mixer | Genereach迷你型微量離心機 | ELISA儀器
澱粉樣β胜肽1-40 (Ab1-40) ELISA試劑盒套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary of the ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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