新型安全DNA/RNA核酸染劑 | Prime Juice Bio-Helix 貨號LD011-1000
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新型安全DNA/RNA核酸染劑 Prime Juice Preloading Fluorescent Stain| Bio-Helix 貨號LD011-1000
靈敏度低至 0.14ng DNA
Bio-Helix提供Prime Juice 新型安全核酸染劑，Prime Juice 具不同激發波長302nm及470nm (不同波長激發效果呈現圖2)，可搭配UV觀察箱(302nm)或藍光觀察箱(470nm)使用；Prime Juice 可代替溴化乙錠(EtBr)是loading dye也是DNA染劑，符合保護環境(local biosafety regulations)並滿足當地生物安全法規/ESG practices/UN SDG Goals 。靈敏度低至 0.14ng DNA與EtBr靈敏度0.1ng以下相當。此安全染劑產品激發(Excitation)波段為302nm或490nm，散射光(Emmision)為525nm (光譜波長如下表)。使用方法為跑電泳時將maker與樣本中加入Prime Juice (1:5倍稀釋)，是一種快速染核之loading dye，不需將膠體預染或外染。快速檢測膠體內雙鏈 DNA (dsDNA)、單鏈 DNA (ssDNA) 和 RNA。
Prime Juice新型安全DNA/RNA核酸染劑使用須知
一般核酸觀察箱分為UV與藍光二種系統；傳統的UV Box觀察箱, 具有長波(365nm)、中波(312nm)、短波(256nm)波段激發。長波能量較弱 (365nm) ，適合切膠等膠體回收(gel extraction)用。短波能量強(256nm)，可以取得較明顯的影像。新一型Safe Blue藍光觀察箱，取代UV的波長為470nm 藍光波段，減少UV光線對樣品及眼睛造成的傷害。搭配不同的核酸觀察箱，Prime Juice新型安全DNA/RNA核酸染劑，將核酸影像最佳化。也減少做實驗誘癌之風險發生。傳統的核酸染劑, EtBr (Biovisioin #1203-10)溴化乙錠 (縮寫為EtBr)，是一種核酸染劑，用為瓊脂糖凝膠電泳後，核酸之染色。在UV紫外光照射下，未結合的ErBr可被激發出橙紅色螢光；結合的DNA或雙股RNA，螢光強度會增強20倍，使得核酸電泳後可分辨核酸相對的分子量。EtBr其激發(Excitation)波段為310nm，散射光(emmision)為570nm。需注意溴化乙錠 (EtBr)為是一種強的誘變劑intercalator，可致癌或致畸。操作時必須佩戴手套。
連續稀釋。正如比較數據所闡明的那樣，在低樣品濃度下，Prime juice 顯示出更好的靈敏度。
Figure 1. Each lane was loaded with 5ul of 100 bp DNA Ladder H3 (DM003-R500) and 1ul of Prime juice (LD011-1000) and Novel Juice (LD001-1000) in serial dilution. At low sample concentrations, Prime juice shows better sensitivity, as elucidated by the comparison data.
圖 2. 兼容性測試
DNA Ladder (DM003-R500, Bio-Helix) 在 2% 瓊脂糖凝膠 (MB755-0100, Bio-Helix) 中運行，TAE 緩衝液載有來自 Lane A-D 的 Prime Juice (LD011-1000, Bio-Helix) 預混物，以及來自 Lane E-H 的 Novel Juice（LD001-1000，Bio-Helix）。凝膠圖像由不同的設備拍攝以顯示兩種染料的外觀。
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
bio-helix.com
Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。
在改善生活質量和實現科學卓越的願望的驅使下，Bio-Helix以最高的誠信標準運營。還將這種精神延伸到我們的服務和全球價值中，以提供給世界各地的客戶。多樣化的員工隊伍和協作網絡形成了公司文化的完美結合。 Bio-Helix 不僅是一家機會均等的公司，而且還努力提供友好和成長的環境。
| CAT# | NAME | Size |
| BP001CU | BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442) |
Set |
| PMI09-0500 | IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa) |
500 μl |
| PMI11-0500 | IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa) |
500 μl |
| PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
500 μl |
| PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
500 μl |
| PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
500 μl |
| PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
500 μl |
| PMB13-0500 | Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa) |
500 μl |
| PMU12-0500 | UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa) |
500 μl |
| CCH321-B100ML | UltraScence Pico Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH345-B100ML | UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH365-B100ML | UltraScence Femto Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH375-B100ML | UltraScence Femto Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| PS002-B500ML | COOMASSIEnano Protein Staining Solution, 500ml | 500 ml |
| PS003-B500ML | Ponceau S Protein Staining Solution | 500 ml |
| BS001-B500ML | OneStep Blocker Western Blocking Solution and Signal Enhancer |
500 ml |
| TM001-B200ML | TMB ELISA Substrate | 100 ml (50ml+50ml) |
| DM001-R500 | BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps) |
500 μl |
| DM003-R500 | BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps) |
500 μl |
| DM012-R500 | BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps) |
500 μl |
| DM015-R500 | BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps) |
500 μl |
| DMF12-0100 | OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye) |
600 μl |
| DMF13-0100 | OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye) |
600 μl |
| LD011-1000 | Prime Juice Preloading Fluorescent Stain | 1 ml |
| LD003-0500 | Novel Green Plus (20,000X) DNA Staining Reagent | 500 μl |
| AGT002-0500 | Agarose Tablets, 110 pcs | 0.5g x 110 |
| MB755-0100 | Agarose Powder 100g -Molecular Biology Grade | 100 g |
| MB755-0500 | Agarose Powder 500g -Molecular Biology Grade | 500 g |
| MB101-0500 | Taq DNA Polymerase | 500U/100μl |
| DP001-0100 | nanoTaq™ Hot-Start DNA Polymerase | 100 rxns 500U/100μl |
| DN001-0250 | 100 mM dNTP Set | 4 x 250μl |
| DN0010 | 10 mM dNTP Mix | 1 ml |
| MB200-P100 | 2X PCR SuperMix | 100 rxns 2 x 1.25mL |
| MBA01-0100 | OmniPCR Supermix w Fluorescent dye | 100 rxns (2.5ml) |
| RK001-0050 | RScript™ cDNA Synthesis Kit | 50 rxns |
| RT001-0050 | RScript™ Reverse Transcriptase 10,000U | 50 rxns 10,000U/50μl |
| RT001-0250 | RScript™ Reverse Transcriptase 50,000U | 250 rxns 50,000U/250μl |
| RI001-0125 | RIBOAssure™ RNase Inhibitor 5,000U | 125 rxns 5,000U/125μl |
| QPR01-0100 | PanProbes™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QPD01-0100 | PanProbes™ Universal qPCR MasterMix | 100 reactions (20 μl vol) |
| QSR01-0100 | PanGreen™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QSD01-0100 | PanGreen™ Universal SYBR® Green Mastermix | 100 reactions (20 μl vol) |
| PDP01-0100 | Plasmid miniPREP Kit | 100 rxns |
| PDP02-0020 | Plasmid midiPREP Kit | 20 rxns |
| PDP03-0010 | Plasmid maxiPREP Kit | 10 rxns |
| PDC01-0100 | PCR Clean-Up & Gel Extraction Kit | 100 rxns |
| PDC02-0100 | Genomic DNA isolation dual kit | 100 rxns |
| PDC05-0100 | Dual Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC06-0100 | Dual Genomic DNA Isolation Kit(Tissue) | 100 rxns |
| PDC09-0100 | Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC10-0100 | Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC11-0100 | Genomic DNA Isolation Kit (Tissue) | 100 rxns |
| PDC12-0100 | Genomic DNA Isolation Kit (Paraffin-embedded tissue) | 100 rxns |
| PDM01-0100 | Mbead Buffy Coat Genomic DNA Kit | 100 rxns |
| PDM02-0100 | Mbead Tissue Genomic DNA Kit | 100 rxns |
| PDM03-0100 | Mbead Bacteria Genomic DNA Kit | 100 rxns |
| PDM05-0100 | Mbead Plant Genomic DNA Kit | 100 rxns |
| PDM06-0100 | Mbead Blood/Cell Genomic DNA Kit | 100 rxns |
| PDR01-0100 | Genomic DNA Isolation Reagent | 100 rxns |
| PDR02-0100 | Plant Genomic DNA Isolation Reagent | 100 rxns |
| PDR05-0100 | Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue) | 100 rxns |
| PDC04-0100 | Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC07-0100 | Total RNA Isolation Kit (Plant) | 100 rxns |
| PDC08-0100 | Total RNA Isolation Kit(Tissue) | 100 rxns |
| PDR03-0100 | Total RNA Isolation Reagent | 100 rxns |
| PDR04-0100 | Plant Total RNA Isolation Reagent | 100 rxns |
| PDC03-0100 | Virus Nucleic Acid Isolation Kit | 100 rxns |
| PDM04-0100 | Mbead Virus Genomic Nucleic Acid Kit | 100 rxns |
| QP019M-0050 | COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa) | 50 rxns |
| QP019T-0100 | COVID-19 RT-qPCR Detection Kit Plus (TFDA) | 100 rxns |
| QP019-0100 | SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO) | 100 rxns |