Plasmid midiPREP Kit | Bio-Helix 貨號PDP02-0020
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Plasmid midiPREP Kit| Bio-Helix 貨號PDP02-0020
Plasmid midiPREP Kit (Up to 250 μg of plasmid)
無需使用苯酚(phenol)、氯仿(chloroform)、溴化乙錠(ethidium bromide)和氯化銫(cesium chloride，從而最大限度地減少有害物質的接觸和處置。 節省時間：不到 40 分鐘即可完成整個過程。
產品描述:
Plasmid midiPREP Kit 提供了一種快速、簡單且經濟高效的質體 midiprep 方法，用於從培養的細菌細胞(cultured bacterial cells)中分離質體粒 DNA( plasmid DN)。 Plasmid midiPREP Kit 基於細菌細胞的鹼性裂解( alkaline lysis )，然後在高鹽存在下將 DNA 結合到離心柱的玻璃纖維基質(glass fiber matrix )上。不需要苯酚提取(Phenol extraction )和乙醇沉澱( ethanol precipitation)，高質量質粒 DNA 在少量 Tris 緩衝液（包含在每個試劑盒中）或水（pH 值在 7.0 和 8.5 之間）中洗脫。使用 Plasmid midiPREP Kit 純化的質粒 DNA 適用於多種常規應用，包括限制性內切酶消化(restriction enzyme digestion)、測序(sequencing)、文庫篩選( library screening)、體外翻譯( in vitro translation)、穩健細胞轉染(transfection of robust cells)、連接(ligation)和轉化(transformation)。整個過程可在 40 分鐘內完成。
Sample: Up to 100 ml bacterial cells
Yield: Up to 250 μg of plasmid
Endotoxin Value: <0.003 EU/μg
Features
- Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, thus minimizing the exposure to and disposal of hazardous materials.
- Time saving: Complete the process in less than 40 minutes.
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
bio-helix.com
Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。
在改善生活質量和實現科學卓越的願望的驅使下，Bio-Helix以最高的誠信標準運營。還將這種精神延伸到我們的服務和全球價值中，以提供給世界各地的客戶。多樣化的員工隊伍和協作網絡形成了公司文化的完美結合。 Bio-Helix 不僅是一家機會均等的公司，而且還努力提供友好和成長的環境。
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| PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
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| PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
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| PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
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| PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
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| DM012-R500 | BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps) |
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