Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus/Bacterial Cells )(Column Based)

產品描述: 

基因組 DNA 分離試劑盒(Genomic DNA Isolation Kit, PDC09-0100)（血液/培養細胞/真菌）專為從全血、冷凍血液、buffy coat、培養的動物/細菌細胞(bacterial cells)和真菌(fungus)中分離基因組 DNA 而設計。這種獨特的緩衝系統 (Optimized lysis buffer) 可確保從樣品中獲得高產量和高質量的基因組 DNA(Genomic DNA)。離心柱(spin column)旨在純化或濃縮先前使用緩衝液分離的基因組 DNA 產品。無需苯酚(phenol)/氯仿(chloroform)提取，整個過程可在 1 小時內完成。純化的基因組 DNA (Purified genomic DNA)適用於 PCR 或其他 enzymatic reactions等下游分析。

樣品需求體積及細胞或細菌數量

Up to 300 µl of the whole blood
Up to 200 µl of the frozen blood
Up to 200 µl of the buffy coat
Cultured animal cells (up to 1 x 107)
Cultured bacterial cells (up to 1 x 109)
Fungus cells (up to 5 x 107)
Format: Reagent and spin column
Yield: Up to 50 μg
Operation time: Within 60 minutes
Elution volume: 50∼200 μl

基因組 DNA 分離試劑盒（血液/培養細胞/真菌）產品特點

 Delivering high-quality genomic DNA with the fast procedure.
 Ready-to-use genomic DNA for high performance in any downstream application.
 Highly purified and high yield genomic DNA can be extracted from various samples.
 Optimized lysis buffer for the efficient lysis.
 Designed to rapidly purify high-quality DNA using spin column format.

Size: 100 Reactions

Required Materials
➢ 1.5 ml Microcentrifuge tubes

➢ RNase A (10 mg/ml) ➢ Absolute ethanol (96~100%)
➢ lysozyme buffer (for Gram-positive Bacteria):
20 mg/ml lysozyme; 20 mM Tris-HCl; 2 mM EDTA; 1% Triton X-100; pH 8.0
➢ Lyticase or zymolase (for fungus)
➢ sorbitol buffer (for fungus):
1.2 M sorbitol;10 mM CaCl2; M Tris-HCl pH 7.5; 35 mM β- mercaptoethanol