麗春紅 S 蛋白染色液|Bio-Helix 貨號PS003-B500ML
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麗春紅 S 蛋白染色液|Bio-Helix 貨號PS003-B500ML
麗春紅Ponceau S-blotting membranes效率可視化染劑 Ponceau S Protein Staining
產品敍述: Ponceau S是一種即用型可逆染色劑。快速檢測硝酸纖維素(nitrocellulose)、PVDF膜、cellulose acetate membranes上蛋白質的染色試劑。操作簡單，在使用Ponceau S溶液反應5分鐘內達到最高染色強度，即可偵測轉漬膜(blotting membrane)上的蛋白條帶 (紅色條帶)， Ponceau S溶液可確認蛋白質經過轉漬後的狀況。蛋白質染劑之染色，可於一抗雜交前進行，確認蛋白膠體是否完全轉漬成功，或轉漬失敗；即可不用浪費進行西方墨點法分析時所需的試劑與抗體。Ponceau S溶液原理為負染色(Negative stain)與蛋白質的帶正電(positively charged)胺基(amino groups)結合；還非共價結合蛋白質中的非極性區域。染色反應為可逆性(reversible)，蛋白質可以進一步進行免疫學檢測( immunological detection)。 Ponceau S允許用戶在Western blotting過程中，membrane blocking 及 protein detection前，確認蛋白質轉移至blotting membranes的效率。此產品與大部份blocking agents具相容性；例如 non-fat dry milk (3-5%)、casein (1%) 及fish skin gelatin (1%)。適用於NC膜、PVDF膜及cellulose acetate membranes；但不建議使用於nylon membranes。在還原和非還原條件下都具有相同的檢測靈敏度和再現性。使用 Ponceau S 染色劑可以快速觀察低至50 ng 的蛋白質。染色後，可以使用0.1% NaOH 中對膜進行脫色。
Ponceau S 溶液可用於評估硝酸纖維素和 PVDF 膜上的總蛋白量或轉移效率。 PVDF 膜對 Ponceau S 染色 5 分鐘，然後用 ddH2O 洗滌 3 分鐘。它提供可見的粉紅色帶。
Lane 1, 13: Prestained Protein Ladder #PM019-0500
Lane 2-7: 2X Dilutions of Unstained Protein Ladder #PMU12-0500
Lane 8-12: 2000, 1000, 500, 100, 50 ng of BSA
- Container: box for gel staining
- Shaker: orbital or rocking shaker
- After the SDS-PAGE Gel has been transferred to a nitrocellulose or PVDF membrane, place the transferred membrane in a staining container.
- Submerge the membrane in a proper amount of Ponceau S Protein Staining Solution that is enough to cover the whole membrane. Lightly agitate the staining container for 5 to 15 minutes at room temperature. Protein bands will be stained and start becoming visible in 5 to 15 minutes.
- Wash away the excessive stain on the membrane with DI water until the protein bands are clear.
- For the further membrane blocking step, remove the Ponceau S Staining Solution from the membrane by placing the membrane in 20 ml 0.1% NaOH for 1 to 2 minutes then rinse in DI water for 1 to 2 minutes.
- The Ponceau S Protein Staining Solution is provided as a ready-to-use solution, there is no need to dilute before use.
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
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| CAT# | NAME | Size |
| BP001CU | BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442) |
Set |
| PMI09-0500 | IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa) |
500 μl |
| PMI11-0500 | IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa) |
500 μl |
| PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
500 μl |
| PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
500 μl |
| PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
500 μl |
| PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
500 μl |
| PMB13-0500 | Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa) |
500 μl |
| PMU12-0500 | UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa) |
500 μl |
| CCH321-B100ML | UltraScence Pico Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH345-B100ML | UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH365-B100ML | UltraScence Femto Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH375-B100ML | UltraScence Femto Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| PS002-B500ML | COOMASSIEnano Protein Staining Solution, 500ml | 500 ml |
| PS003-B500ML | Ponceau S Protein Staining Solution | 500 ml |
| BS001-B500ML | OneStep Blocker Western Blocking Solution and Signal Enhancer |
500 ml |
| TM001-B200ML | TMB ELISA Substrate | 100 ml (50ml+50ml) |
| DM001-R500 | BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps) |
500 μl |
| DM003-R500 | BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps) |
500 μl |
| DM012-R500 | BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps) |
500 μl |
| DM015-R500 | BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps) |
500 μl |
| DMF12-0100 | OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye) |
600 μl |
| DMF13-0100 | OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye) |
600 μl |
| LD011-1000 | Prime Juice Preloading Fluorescent Stain | 1 ml |
| LD003-0500 | Novel Green Plus (20,000X) DNA Staining Reagent | 500 μl |
| AGT002-0500 | Agarose Tablets, 110 pcs | 0.5g x 110 |
| MB755-0100 | Agarose Powder 100g -Molecular Biology Grade | 100 g |
| MB755-0500 | Agarose Powder 500g -Molecular Biology Grade | 500 g |
| MB101-0500 | Taq DNA Polymerase | 500U/100μl |
| DP001-0100 | nanoTaq™ Hot-Start DNA Polymerase | 100 rxns 500U/100μl |
| DN001-0250 | 100 mM dNTP Set | 4 x 250μl |
| DN0010 | 10 mM dNTP Mix | 1 ml |
| MB200-P100 | 2X PCR SuperMix | 100 rxns 2 x 1.25mL |
| MBA01-0100 | OmniPCR Supermix w Fluorescent dye | 100 rxns (2.5ml) |
| RK001-0050 | RScript™ cDNA Synthesis Kit | 50 rxns |
| RT001-0050 | RScript™ Reverse Transcriptase 10,000U | 50 rxns 10,000U/50μl |
| RT001-0250 | RScript™ Reverse Transcriptase 50,000U | 250 rxns 50,000U/250μl |
| RI001-0125 | RIBOAssure™ RNase Inhibitor 5,000U | 125 rxns 5,000U/125μl |
| QPR01-0100 | PanProbes™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QPD01-0100 | PanProbes™ Universal qPCR MasterMix | 100 reactions (20 μl vol) |
| QSR01-0100 | PanGreen™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QSD01-0100 | PanGreen™ Universal SYBR® Green Mastermix | 100 reactions (20 μl vol) |
| PDP01-0100 | Plasmid miniPREP Kit | 100 rxns |
| PDP02-0020 | Plasmid midiPREP Kit | 20 rxns |
| PDP03-0010 | Plasmid maxiPREP Kit | 10 rxns |
| PDC01-0100 | PCR Clean-Up & Gel Extraction Kit | 100 rxns |
| PDC02-0100 | Genomic DNA isolation dual kit | 100 rxns |
| PDC05-0100 | Dual Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC06-0100 | Dual Genomic DNA Isolation Kit(Tissue) | 100 rxns |
| PDC09-0100 | Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC10-0100 | Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC11-0100 | Genomic DNA Isolation Kit (Tissue) | 100 rxns |
| PDC12-0100 | Genomic DNA Isolation Kit (Paraffin-embedded tissue) | 100 rxns |
| PDM01-0100 | Mbead Buffy Coat Genomic DNA Kit | 100 rxns |
| PDM02-0100 | Mbead Tissue Genomic DNA Kit | 100 rxns |
| PDM03-0100 | Mbead Bacteria Genomic DNA Kit | 100 rxns |
| PDM05-0100 | Mbead Plant Genomic DNA Kit | 100 rxns |
| PDM06-0100 | Mbead Blood/Cell Genomic DNA Kit | 100 rxns |
| PDR01-0100 | Genomic DNA Isolation Reagent | 100 rxns |
| PDR02-0100 | Plant Genomic DNA Isolation Reagent | 100 rxns |
| PDR05-0100 | Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue) | 100 rxns |
| PDC04-0100 | Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC07-0100 | Total RNA Isolation Kit (Plant) | 100 rxns |
| PDC08-0100 | Total RNA Isolation Kit(Tissue) | 100 rxns |
| PDR03-0100 | Total RNA Isolation Reagent | 100 rxns |
| PDR04-0100 | Plant Total RNA Isolation Reagent | 100 rxns |
| PDC03-0100 | Virus Nucleic Acid Isolation Kit | 100 rxns |
| PDM04-0100 | Mbead Virus Genomic Nucleic Acid Kit | 100 rxns |
| QP019M-0050 | COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa) | 50 rxns |
| QP019T-0100 | COVID-19 RT-qPCR Detection Kit Plus (TFDA) | 100 rxns |
| QP019-0100 | SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO) | 100 rxns |