未染色蛋白分子量標準（10 to 200 kDa）|Bio-Helix 貨號PMU12-0500
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未染色蛋白分子量標準（10 to 200 kDa）|Bio-Helix 貨號PMU12-0500
UNveil Unstained Protein Ladder（10 to 200 kDa）SIZE: 500 μl
產品描述:
The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Storage Buffer :
20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.
Quality Control :
Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.
Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Note. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
All products are for research use only.
Caution :
Not intended for human or animal diagnostic or therapeutic uses.
Lane 2: PM008-0500 (Bio-Helix) 5ul
Lane 3: PM007-0500 (Bio-Helix) 5ul
Lane 4: PM006-0500 (Bio-Helix) 5ul
Lane 5: PM001-0500 (Bio-Helix) 5ul
Lane 6: PM019-0500 (Bio-Helix) 5ul
Lane 7: PM001-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
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Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。
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| PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
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| PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
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| PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
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| PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
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500 μl |
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500 μl |
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500 μl |
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| DP001-0100 | nanoTaq™ Hot-Start DNA Polymerase | 100 rxns 500U/100μl |
| DN001-0250 | 100 mM dNTP Set | 4 x 250μl |
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| MBA01-0100 | OmniPCR Supermix w Fluorescent dye | 100 rxns (2.5ml) |
| RK001-0050 | RScript™ cDNA Synthesis Kit | 50 rxns |
| RT001-0050 | RScript™ Reverse Transcriptase 10,000U | 50 rxns 10,000U/50μl |
| RT001-0250 | RScript™ Reverse Transcriptase 50,000U | 250 rxns 50,000U/250μl |
| RI001-0125 | RIBOAssure™ RNase Inhibitor 5,000U | 125 rxns 5,000U/125μl |
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| QPD01-0100 | PanProbes™ Universal qPCR MasterMix | 100 reactions (20 μl vol) |
| QSR01-0100 | PanGreen™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QSD01-0100 | PanGreen™ Universal SYBR® Green Mastermix | 100 reactions (20 μl vol) |
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| PDP02-0020 | Plasmid midiPREP Kit | 20 rxns |
| PDP03-0010 | Plasmid maxiPREP Kit | 10 rxns |
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| PDC06-0100 | Dual Genomic DNA Isolation Kit(Tissue) | 100 rxns |
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| PDC10-0100 | Genomic DNA Isolation Kit (Plant) | 100 rxns |
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| PDR02-0100 | Plant Genomic DNA Isolation Reagent | 100 rxns |
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| PDC04-0100 | Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
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| PDC08-0100 | Total RNA Isolation Kit(Tissue) | 100 rxns |
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