植物總RNA分離試劑（試劑基）|Bio-Helix 貨號PDR04-0100
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植物總RNA分離試劑（試劑基）|Bio-Helix 貨號PDR04-0100
Plant Total RNA Isolation Reagent (Reagent Based)
The Plant Total RNA Isolation Reagent provides an easy 3-step method to isolate the total RNA from plant samples. This unique reagent system ensures the total RNA with a high yield and good quality from the most common plant samples as well as samples high in polysaccharides. If a larger sample is required, the kit volume can be scaled proportionately, making the kit not only very user-friendly but also highly versatile. The RNA phenol extraction is not required, and the entire procedure can be completed in 2 hours. The total RNA (up to 80 μg for fresh plant tissue) is ready for use in RT-PCR, Northern Blotting, cDNA Synthesis and Mapping.
產品描述:
植物 RNA（和 DNA）提取過程中，物種器官或植物組織來源不同，RNA的表現可能不同。植物多樣性包括存在大量多醣( polysaccharides)、高 RNase 水平、酚類物質(phenolics)，包括單寧酸( tannins)、低核酸(nucleic acid)濃度（高水含量）和組織，如木質素（木材）等影響DNA和RNA 質量，並抑制下游反應。Bio-Helix 提供植物總RNA分離和純化試劑盒。獨特優化試劑系統確保高多醣樣品中獲得高產量和高質量的總 RNA。無需RNA苯酚提取，整個過程可在2小時內完成。總 RNA（新鮮植物組織高達 80 μg）可用於 RT-PCR、Northern 印跡、cDNA 合成和Mapping。
植物總RNA分離試劑（試劑基）產品特點
• Fast procedure delivering high-quality total RNA.
• Ready-to-use RNA for high performance in any downstream application.
• Consistent RNA yield from the starting material with a small amount.
• Provides sufficient reagents and 3 steps to treat samples.
PR Total RNA Isolation Protocol
Sample Preparation
1.Cut off 100 mg of the fresh plant tissue or 50 mg of the dry plant tissue.
2.Grind the sample in the liquid nitrogen to a fine powder using a mortar and pestle.
Step 1 Lysis
1. Add 1 ml of the PR buffer 1 and 12 µl of the ß-mercaptoethanol to the sample in the mortar and grind the sample until it is completely dissolved.
2. Transfer the dissolved sample to a 1.5 ml microcentrifuge tube.
3. Incubate at 70°C for 50 minutes.
4. Incubate at 15~30°C for 5 minutes.
5. Centrifuge at 2~8°C at 14-16,000 x g for 15 minutes.
6. Transfer the supernatant to a new 1.5 ml microcentrifuge tube.
Step 2 Phase Separation
1. Add a 1/10 volume of the PR buffer 2 and 500 μl of the chloroform to the supernatant from the Step 1.
2. Shake vigorously and then centrifuge at 2~8°C at 14~16,000 x g for 10 minutes.
3. Carefully remove the upper phase and transfer it to a new 1.5 ml microcentrifuge tube tube.
4. Repeat the Phase Separation Step until the interphase becomes clear, and then transfer the clear upper phase to a new 1.5 ml microcentrifuge tube.
Step 3 RNA Precipitation
1. Add 500 μl of the isopropanol to the 1.5 ml microcentrifuge tube containing the clear upper phase from the Step 2.
2. Mix the sample by inverting gently and incubating on the ice for 10 minutes.
3. Centrifuge at 2~8°C at 14~16,000 x g for 15 minutes.
4. Discard the supernatant and wash the pellet with 1 ml of the 70% EtOH.
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
乾浴加熱水浴槽 | Finnpipette F3 微量分注器 | 試管振盪器 Vortex Mixer | Genereach迷你型微量離心機 | ELISA儀器
bio-helix.com
Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。
在改善生活質量和實現科學卓越的願望的驅使下，Bio-Helix以最高的誠信標準運營。還將這種精神延伸到我們的服務和全球價值中，以提供給世界各地的客戶。多樣化的員工隊伍和協作網絡形成了公司文化的完美結合。 Bio-Helix 不僅是一家機會均等的公司，而且還努力提供友好和成長的環境。
| CAT# | NAME | Size |
| BP001CU | BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442) |
Set |
| PMI09-0500 | IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa) |
500 μl |
| PMI11-0500 | IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa) |
500 μl |
| PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
500 μl |
| PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
500 μl |
| PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
500 μl |
| PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
500 μl |
| PMB13-0500 | Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa) |
500 μl |
| PMU12-0500 | UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa) |
500 μl |
| CCH321-B100ML | UltraScence Pico Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH345-B100ML | UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH365-B100ML | UltraScence Femto Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH375-B100ML | UltraScence Femto Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| PS002-B500ML | COOMASSIEnano Protein Staining Solution, 500ml | 500 ml |
| PS003-B500ML | Ponceau S Protein Staining Solution | 500 ml |
| BS001-B500ML | OneStep Blocker Western Blocking Solution and Signal Enhancer |
500 ml |
| TM001-B200ML | TMB ELISA Substrate | 100 ml (50ml+50ml) |
| DM001-R500 | BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps) |
500 μl |
| DM003-R500 | BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps) |
500 μl |
| DM012-R500 | BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps) |
500 μl |
| DM015-R500 | BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps) |
500 μl |
| DMF12-0100 | OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye) |
600 μl |
| DMF13-0100 | OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye) |
600 μl |
| LD011-1000 | Prime Juice Preloading Fluorescent Stain | 1 ml |
| LD003-0500 | Novel Green Plus (20,000X) DNA Staining Reagent | 500 μl |
| AGT002-0500 | Agarose Tablets, 110 pcs | 0.5g x 110 |
| MB755-0100 | Agarose Powder 100g -Molecular Biology Grade | 100 g |
| MB755-0500 | Agarose Powder 500g -Molecular Biology Grade | 500 g |
| MB101-0500 | Taq DNA Polymerase | 500U/100μl |
| DP001-0100 | nanoTaq™ Hot-Start DNA Polymerase | 100 rxns 500U/100μl |
| DN001-0250 | 100 mM dNTP Set | 4 x 250μl |
| DN0010 | 10 mM dNTP Mix | 1 ml |
| MB200-P100 | 2X PCR SuperMix | 100 rxns 2 x 1.25mL |
| MBA01-0100 | OmniPCR Supermix w Fluorescent dye | 100 rxns (2.5ml) |
| RK001-0050 | RScript™ cDNA Synthesis Kit | 50 rxns |
| RT001-0050 | RScript™ Reverse Transcriptase 10,000U | 50 rxns 10,000U/50μl |
| RT001-0250 | RScript™ Reverse Transcriptase 50,000U | 250 rxns 50,000U/250μl |
| RI001-0125 | RIBOAssure™ RNase Inhibitor 5,000U | 125 rxns 5,000U/125μl |
| QPR01-0100 | PanProbes™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QPD01-0100 | PanProbes™ Universal qPCR MasterMix | 100 reactions (20 μl vol) |
| QSR01-0100 | PanGreen™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QSD01-0100 | PanGreen™ Universal SYBR® Green Mastermix | 100 reactions (20 μl vol) |
| PDP01-0100 | Plasmid miniPREP Kit | 100 rxns |
| PDP02-0020 | Plasmid midiPREP Kit | 20 rxns |
| PDP03-0010 | Plasmid maxiPREP Kit | 10 rxns |
| PDC01-0100 | PCR Clean-Up & Gel Extraction Kit | 100 rxns |
| PDC02-0100 | Genomic DNA isolation dual kit | 100 rxns |
| PDC05-0100 | Dual Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC06-0100 | Dual Genomic DNA Isolation Kit(Tissue) | 100 rxns |
| PDC09-0100 | Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC10-0100 | Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC11-0100 | Genomic DNA Isolation Kit (Tissue) | 100 rxns |
| PDC12-0100 | Genomic DNA Isolation Kit (Paraffin-embedded tissue) | 100 rxns |
| PDM01-0100 | Mbead Buffy Coat Genomic DNA Kit | 100 rxns |
| PDM02-0100 | Mbead Tissue Genomic DNA Kit | 100 rxns |
| PDM03-0100 | Mbead Bacteria Genomic DNA Kit | 100 rxns |
| PDM05-0100 | Mbead Plant Genomic DNA Kit | 100 rxns |
| PDM06-0100 | Mbead Blood/Cell Genomic DNA Kit | 100 rxns |
| PDR01-0100 | Genomic DNA Isolation Reagent | 100 rxns |
| PDR02-0100 | Plant Genomic DNA Isolation Reagent | 100 rxns |
| PDR05-0100 | Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue) | 100 rxns |
| PDC04-0100 | Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC07-0100 | Total RNA Isolation Kit (Plant) | 100 rxns |
| PDC08-0100 | Total RNA Isolation Kit(Tissue) | 100 rxns |
| PDR03-0100 | Total RNA Isolation Reagent | 100 rxns |
| PDR04-0100 | Plant Total RNA Isolation Reagent | 100 rxns |
| PDC03-0100 | Virus Nucleic Acid Isolation Kit | 100 rxns |
| PDM04-0100 | Mbead Virus Genomic Nucleic Acid Kit | 100 rxns |
| QP019M-0050 | COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa) | 50 rxns |
| QP019T-0100 | COVID-19 RT-qPCR Detection Kit Plus (TFDA) | 100 rxns |
| QP019-0100 | SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO) | 100 rxns |