Mouse Anti-Human IgE Fc-Alexa Fluor® 555抗體 | 貨號9160-32  規格0.2 ml  (0.5 mg/mL)

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

產品介紹:  Mouse Anti-Human IgE Fc-Alexa Fluor® 555抗體，具Alexa Fluor® 555標誌。針對Human IgE Fc具特異性。Isotype為Mouse IgG₁κ。緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑。

ELISA plate was coated with purified human IgG, IgM, IgA, and IgE. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgE Fc-HRP (SB Cat. No. 9160-05).

Clone	B3102E8
Isotype	Mouse IgG1κ
Specificity	Human IgE Fc
Format/Conjugate	AF555 (Alexa Fluor® 555)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	0.2 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – ELISA – Quality tested 1-3 FLISA – Quality tested Flow Cytometry – Reported in literature 16 Immunohistochemistry-Frozen Sections – Reported in literature 4-13 Immunocytochemistry – Reported in literature 12 Western Blot – Reported in literature 2,3,12,14,15
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796673

Cat. No.	Product Description	Clone	Size
9160-32	Mouse Anti-Human IgE Fc-Alexa Fluor® 555	B3102E8	0.1 mg
9160-31	Mouse Anti-Human IgE Fc-Alexa Fluor® 647	B3102E8	0.1 mg
9160-04	Mouse Anti-Human IgE Fc-AP	B3102E8	1.0 mL
9160-08	Mouse Anti-Human IgE Fc-BIOT	B3102E8	0.5 mg
9160-02	Mouse Anti-Human IgE Fc-FITC	B3102E8	0.5 mg
9160-05	Mouse Anti-Human IgE Fc-HRP	B3102E8	1.0 mL
9160-01	Mouse Anti-Human IgE Fc-UNLB	B3102E8	0.5 mg

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

引用文獻

References

1. Xue L, Johnson R, Gorovits B. Prevalence and isotypic complexity of the anti-Chinese hamster ovary host cell protein antibodies in normal human serum. AAPS J. 2010;12:98-106. (ELISA)
2. Goodman RE, Panda R, Ariyarathna H. Evaluation of endogenous allergens for the safety evaluation of genetically engineered food crops: review of potential risks, test methods, examples and relevance. J Agric Food Chem. 2013;61:8317-32. (ELISA, WB)
3. Berkner H, Seutter von Loetzen C, Hartl M, Randow S, Gubesch M, Vogel L, et al. Enlarging the toolbox for allergen epitope definition with an allergen-type model protein. PLoS One. 2014;9(10):e111691. (ELISA, WB)
4. Meding B, Ahonen K, Brisman J, Hedblad M, Ståhl A, Järvholm B, et al. Late skin-prick-test reactions to malted wheat. Clinical observations and immunohistochemical characterization. Allergy. 1998;53:282-8. (IHC-FS)
5. Tengvall Linder M, Johansson C, Scheynius A, Wahlgren C. Positive atopy patch test reactions to Pityrosporum orbiculare in atopic dermatitis patients. Clin Exp Allergy. 2000;30:122-31. (IHC-FS)
6. Åström K, Magnusson CG, Papatziamos G, Hemlin C, Scheynius A, van der Ploeg I, et al. Analysis of epsilon germline transcripts and IL-4 mRNA expression in the adenoids suggests local IgE switching. Allergy. 2000;55:1049-55. (IHC-FS)
7. Sverremark Ekström E, Nilsson C, Holmlund U, van der Ploeg I, Sandstedt B, Lilja G, et al. IgE is expressed on, but not produced by, fetal cells in the human placenta irrespective of maternal atopy. Clin Exp Immunol. 2002;127:274-82. (IHC-FS)
8. Nivenius E, Montan PG, Chryssanthou E, Jung K, van Hage-Hamsten M, van der Ploeg I. No apparent association between periocular and ocular microcolonization and the degree of inflammation in patients with atopic keratoconjunctivitis. Clin Exp Allergy. 2004;34:725-30. (IHC-FS)
9. Rindsjö E, Varli IH, Ofori MF, Lundquist M, Holmlund U, Papadogiannakis N, et al. Presence of IgE+ cells in human placenta is independent of malaria infection or chorioamnionitis. Clin Exp Immunol. 2006;144:204-11. (IHC-FS)
10. Forshammar J, Isaksson S, Strid H, Stotzer P, Sjövall H, Simrén M, et al. A pilot study of colonic B cell pattern in irritable bowel syndrome. Scand J Gastroenterol. 2008;43:1461-66. (IHC-FS)
11. Spazierer D, Skvara H, Dawid M, Fallahi N, Gruber K, Rose K, et al. T helper 2 biased de novo immune response to Keyhole Limpet Hemocyanin in humans. Clin Exp Allergy. 2009;39:999-1008. (IHC-FS)
12. Altrichter S, Kriehuber E, Moser J, Valenta R, Kopp T, Stingl G. Serum IgE autoantibodies target keratinocytes in patients with atopic dermatitis. J Invest Dermatol. 2008;128:2232-9. (IHC-FS, ICC, WB)
13. Joerink M, Rindsjö E, Stenius F, Alm J, Lilja G, Grönlund H, et al. Evidence for allergen-specific IgE of maternal origin in human placenta. Allergy. 2009;64:905-12. (IHC-FS)
14. Gómez-Ollés S, Cruz MJ, Renström A, Doekes G, Morell F, Rodrigo MJ. An amplified sandwich EIA for the measurement of soy aeroallergens. Clin Exp Allergy. 2006;36:1176-83. (WB)
15. Venkatachalam M, Teuber SS, Peterson WR, Roux KH, Sathe SK. Antigenic stability of pecan [Carya illinoinensis (Wangenh.) K. Koch] proteins: effects of thermal treatments and in vitro digestion. J Agric Food Chem. 2006;54:1449-58. (WB)
16. Buentke E, Zargari A, Heffler LC, Avila-Cariño J, Savolainen J, Scheynius A. Uptake of the yeast Malassezia furfur and its allergenic components by human immature CD1a+ dendritic cells. Clin Exp Allergy. 2000;30:1759-70. (FC)