蛋白凍緩衝液 | Buffered Peptone Water (BPW) (ISO)貨號ncm0015

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6公升迷你培養箱 | 菌液測量 (分光光度計) | 滅菌釜 | 水浴槽WaterBath | 電子天平 | pH酸鹼度計 | Vortex | Pipet aid | Petri dishes 培養皿
食品微生物之檢驗方法-沙門氏菌之檢驗 (Salmonella spp.)
蛋白凍緩衝液 | Buffered Peptone Water (BPW) (ISO)貨號ncm0015
產品介紹:緩衝蛋白腖水(Buffered Peptone Water, BPW)(ISO)用於食品中多種微生物的非選擇性預混緩衝液,包括沙門氏菌(Salmonella spp)和克羅諾桿菌屬(Cronobacter spp)。。 不適用於人類疾病或其他疾病的診斷。緩衝蛋白腖水(Buffered Peptone Water, BPW)(ISO)根據ISO 6579-1:2017,ISO 22694:2017,ISO 21528-1:2017和ISO 12950:2010制定。這種非選擇性預增菌培養基旨在幫助將亞致死的沙門氏菌恢復到選擇培養基之前的狀態。不含抑制劑,並具有良好的緩衝作用,可在孵育期間保持pH 7.0。對沙門氏菌的亞致死性傷害發生在許多食品加工過程中,pre-enrichment步驟大大提高了這些生物的回收率。
- For detection of Salmonella spp. (Water Quality) – Refer to ISO 19250:2010
- For detection and enumeration of Enterobacteriaceae – Refer to ISO 21528-1:2017
- For detection and enumeration and Serotyping of Salmonella – Refer to ISO 6579-1:2017
- For detection Cronobacter spp. – Refer to ISO 22964:2017
- For detection of STEC – Refer to ISO/TS 13136:2012
培養基使用方法: 將20.1克培養基溶於1升純淨水中。如有必要,經常攪拌加熱以完全溶解Medium。在121℃下高壓滅菌15分鐘。等冷去後,最終pH:7.0±0.2, 25℃分裝於Petri dish培養皿中,檢驗方法:檢體經系列稀釋後,以平板計數培養基培養及計數之方法。 2.1. 工作環境:工作平台須寬敞、潔淨、光線良好,操作平台光度為 100 呎燭光以上,密閉室內換氣良好,儘可能沒有灰塵及流 動空氣。每 15 分鐘落菌數不得超過 15 CFU/培養皿。
| Formula | Liter |
| Enzymatic Digest of Casein | 10.0 g/L |
| Sodium Chloride | 5.0 g/L |
| Disodium Hydrogen Phosphate (anhydrous) | 3.6 g/L |
| Potassium Dihydrogen Phosphate | 1.5 g/L |
文獻參考References
1. ISO 19250:2010 Water quality — Detection of Salmonella spp.
2. ISO 21528-1:2017 Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 1: Detection of Enterobacteriaceae
3. ISO 6579-1:2017 Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella
4. ISO 22964:2017 Microbiology of the food chain — Horizontal method for the detection of Cronobacter spp.
5. ISO/TS 13136:2012 Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
更多資料來自FDA生菌數之檢驗 (參考方法)
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