LB Broth (Miller)培養基| Escherichia coli DH5 a培養基 Neogen貨號LAB169 / NCM0088A
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特點
6公升迷你培養箱 | 菌液測量 (分光光度計) | 滅菌釜 | 水浴槽WaterBath | 電子天平 | pH酸鹼度計 | Vortex | Pipet aid | Petri dishes 培養皿
微生物之檢驗方法－Escherichia coli DH5 a培養
LB Broth (Miller)| 貨號LAB169 / NCM0088A
LB培養基（Miller）（Lysogeny broth，LB），是微生物學實驗中最常用的營養性培養基，用於培養大腸桿菌等細菌之生長和維持。分為液態培养基和加入瓊脂製成的固態培養基。Minimum QC organisms: Escherichia coli DH5 a(ATCC® 53868)。LB Broth(Miller)培養基用於分子微生物學程序中使用的大腸桿菌菌株的生長和維持，營養豐富，旨在用於重組菌株純培養物的生長。大腸桿菌在LB培養基中生長至對數後期(late log phase)。一些vectors載體無需選擇性擴增(selectively amplified) 即可複製至高拷貝數(high copy numbers)。一些載體不能自由複製，需要選擇性擴增。可以加入氯黴素(Chloramphenicol)以抑制宿主合成並防止細菌染色體複製。LB Broth(Miller)培養基含有高含量的氯化鈉(sodium chloride)，有助於維持plasmids。如果使用lambda噬菌體 (temperate bacteriophages)，建議添加2g / l的硫酸鎂(magnesium sulphate)以促進噬菌體吸收(phage absorption)。如果需要，可以在滅菌後對培養基進行無菌葡萄糖(glucose)補充。
酵素萃取物Yeast Extract ————————-5.0g / L
酪蛋白酶消化(Enzymatic Digest of Casein )————————-10.0g / L
Sodium Chloride——————————————————-10.0g / L
蒸餾水 _________________1000ml
，經 121℃滅菌 15 分鐘，最 後 pH 值為7.3 ± 0.2。
將25克培養基溶於1升純淨水中。如有必要，經常攪拌加熱以完全溶解Medium。在121℃下高壓滅菌15分鐘。等冷去後，最終pH：7.3 ± 0.2, 25℃分裝於Petri dish培養皿中。
檢驗方法：檢體經系列稀釋後，以平板計數培養基培養及計數之方法。 2.1. 工作環境：工作平台須寬敞、潔淨、光線良好，操作平台光度為 100 呎燭光以上，密閉室內換氣良好，儘可能沒有灰塵及流 動空氣。每 15 分鐘落菌數不得超過 15 CFU/培養皿。更多資料來自FDA生菌數之檢驗 (參考方法)。
預期的培養反應：Bacillus subtilis ATCC® 9372、 Escherichia coli ATCC® 25922、 Escherichia coli ATCC® 33876、 Escherichia coli ATCC® 39403 、 Escherichia coli ATCC® 47014 、Staphylococcus aureus ATCC® 25923 在適當的氣氛和溫度下在 LB Broth Miller 中進行培養反應，並在孵育 18 – 24 小時後檢查生長情況。
References
1. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York.
2. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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| 貨號 | 品項 |
| NCM0001A | Half Fraser (Demi-Fraser) Broth (ISO) 500g |
| NCM0002A | Tryptic Soy Agar (Soybean-Casein Digest Agar) 500g |
| NCM0003A | Buffered Peptone Water (BPW) 500g |
| NCM0004A | Tryptic Soy Broth (Soybean-Casein Digest Broth) 500g |
| NCM0005A | Lactose Broth 500g |
| NCM0007A | NFL Aseptic Validation Medium 500g |
| NCM0008A | Sabouraud Dextrose Agar 500g |
| NCM0009A | D/E Neutralizing Agar with Tween 500g |
| NCM0010A | Plate Count Agar (Standard Methods) 500g |
| NCM0011A | Tryptic Soy Agar with Lecithin and Tween 80 500g |
| NCM0012A | UVM Broth 500g |
| NCM0013A | Columbia Agar 500g |
| NCM0014A | Fastidious Anaerobe Agar 500g |
| NCM0015A | Buffered Peptone Water (BPW) (ISO) 500g |
| NCM0016A | Brain Heart Infusion Broth 500g |
| NCM0017A | MacConkey Agar 500g |
| NCM0018A | Potato Dextrose Agar 500g |
| NCM0019A | Tryptone Soy Broth 500g |
| NCM0020A | Tryptone Soy Agar 500g |
| NCM0021A | Xylose Lysine Deoxycholate (XLD) Agar (ISO) 500g |
| NCM0022A | Violet Red Bile Glucose Agar (VRBGA) 500g |
| NCM0023A | Mueller Hinton Agar II 500g |
| NCM0024A | Baird-Parker Agar 500g |
| NCM0025A | Violet Red Bile Agar 500g |
| NCM0026A | Tryptic Soy Broth, Modified with Acid Digest of Casein 500g |
| NCM0027A | Xylose Lysine Deoxycholate (XLD) Agar 500g |
| NCM0028A | Corn Meal Agar 500g |
| NCM0029A | DRBC Agar (BAM) 500g |
| NCM0030A | Lauryl Sulfate Broth 500g |
| NCM0031A | Columbia Blood Agar Base 500g |
| NCM0032A | Lauryl Tryptose (LST) Broth 500g |
| NCM0033A | Nutrient Agar 500g |
| NCM0034A | Cystine Lactose Electrolyte Deficient (CLED) Agar 500g |
| NCM0035A | Lactobacilli MRS Agar 500g |
| NCM0037A | BCYE Agar (Legionella Isolation Medium) 500g |
| NCM0038A | Columbia Agar Base 500g |
| NCM0039A | Membrane Lauryl Sulphate Broth (500 g) |
| NCM0040A | Blood Agar Base No. 2 500g |
| NCM0041A | Violet Red Bile Glucose Agar (ISO) 500g |
| NCM0042A | Campy Blood-Free Selective Medium (Modified CCDA) – 500g |
| NCM0043A | Middlebrook 7H11 Agar 500g |
| NCM0044A | Universal Preenrichment Broth 500g |
| NCM0045A | GC Agar 500g |
| NCM0046A | Salmonella Shigella (SS) Agar 500g |
| NCM0047A | D/E Neutralizing Broth with Tween 500g |
| NCM0048A | Brilliant Green Bile 2% Broth – 500g |
| NCM0048A | Brilliant Green Bile 2% Broth – 500g |
| NCM0049A | PALCAM Broth – 500g |
| NCM0050A | Fraser Broth 500g |
| NCM0051A | Buffered Listeria Enrichment Broth 500g |
| NCM0052A | Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar 500g |
| NCM0053A | Acutone PPLO Broth w/o Crystal Violet – 500g |
| NCM0054A | Orange Serum Agar 500g |
| NCM0055A | Listeria Enrichment Broth 500g |
| NCM0056A | Oxford Listeria Agar 500g |
| NCM0058A | Brilliant Green Agar (Modified) 500g |
| NCM0059A | YM Broth – 500g |
| NCM0060A | MacConkey Broth 500g |
| NCM0061A | Todd Hewitt Broth 500g |
| NCM0062A | Bacillus cereus MYP Agar – 500g |
| NCM0063A | Tryptic Soy Broth, Modified with Novobiocin 500g |
| NCM0064A | Violet Red Bile Agar with MUG (VBRA-MUG) 500g |
| NCM0065A | EC Broth 500g |
| NCM0066A | Fraser Broth Base 500g |
| NCM0067A | Modified Semi-solid Rappaport-Vassiladis (MSRV) Agar 500g |
| NCM0068A | Sabouraud Dextrose Agar with Chloramphenicol 500g |
| NCM0069A | Yeast Extract Agar 500g |
| NCM0070A | Eugon Broth 500g |
| NCM0071A | Lauryl Sulfate Broth w/ MUG – 500g |
| NCM0072A | MacConkey Agar without Crystal Violet and Salt 500g |
| NCM0073A | Dextrose Tryptone Broth – 500g |
| NCM0074A | KF Streptococcus Agar – 500g |
| NCM0075A | Blood Agar Base 500g |
| NCM0076A | R2A Agar 500g |
| NCM0077A | Perfrigens Agar Base (TSC) 500g |
| NCM0078A | Mannitol Salt Agar – 500g |
| NCM0079A | Lactobacilli MRS Broth 500g |
| NCM0080A | Brain Heart Infusion Agar – 500g |
| NCM0081A | Dichloran Glycerol (DG-18) Agar Base 500g |
| NCM0082A | DRBC Agar (ISO) 500g |
| NCM0083A | Pseudomonas Agar Base 500g |
| NCM0084A | Modified Buffered Peptone Water with Pyruvate – 500g |
| NCM0085A | Maximum Recovery Diluent (Tryptone Salt Broth) 500g |
| NCM0086A | Bismuth Sulfite Agar 500g |
| NCM0087A | Tryptose Broth – 500g |
| NCM0088A | LB Broth (Miller) 500g |
| NCM0089A | Violet Red Bile Agar (VRBA) (ISO) – 500g |
| NCM0090A | Brucella Agar – 500g |
| NCM0091A | TAT Broth – 500g |
| NCM0092A | Tetrathionate Broth Base 500g |
| NCM0093A | Malt Extract Agar 500g |
| NCM0094A | Campylobacter Enrichment Broth (Bolton Broth) 500g |
| NCM0095A | Sabouraud Dextrose Agar (with Lecithin & Tween) – 500g |
| NCM0096A | Peptone Water 500g |
| NCM0098A | Tryptic Soy Blood Agar Base No. 2 – 500g |
| NCM0099A | Campy Cefex Agar – 500g |
| NCM0101A | TSB, Modified w/ 8mg/L Novobiocin & ADC – 500g |
| NCM0102A | Reinforced Clostridial Medium 500g |
| NCM0103A | Rappaport Vassiliadis Salmonella Enrichment Broth 500g |
| NCM0104A | Letheen Broth with Tween, Modified – 500g |
| NCM0105A | Eosin Methylene Blue (EMB) Agar Levine 500g |
| NCM0106A | Wilkins-Chalgren Agar – 500g |
| NCM0107A | EC Medium w/ MUG – 500g |
| NCM0108A | Fluid Thioglycollate Medium 500g |
| NCM0109A | Cetrimide Agar 500g |
| NCM0110A | Nutrient Broth 500g |
| NCM0111A | Palcam Agar Base 500g |
| NCM0112A | DC Medium with BCIG – 500g |
| NCM0113A | PPLO Broth Base without Crystal Violet 500g |
| NCM0115A | Columbia CNA Agar – 500g |
| NCM0116A | Letheen Broth (with Tween) – 500g |
| NCM0117A | Bile Esculin Agar 500g |
| NCM0118A | WL Nutrient Agar 500g |
| NCM0119A | Milk Plate Count Agar 500g |
| NCM0120A | Casein Peptone Type I – 500g |
| NCM0121A | D/E Neutralizing Broth without Tween 500g |
| NCM0122A | Presence Absence Broth – 500g |
| NCM0123A | m-Endo Agar – 500g |
| NCM0124A | A-1 Medium – 500g |
| NCM0125A | M Broth – 500g |
| NCM0126A | Muller-Kauffmann Tetrathionate-Novobiocin (MKTTn) Broth 5 |
| NCM0127A | Dextrose Tryptone Agar 500g |
| NCM0128A | Clostridium Diffcile Agar Base (Brazier’s) 500g |
| NCM0129A | Letheen Broth Base Modified without Tween – 500g |
| NCM0130A | Letheen Agar Base Modified without Tween – 500g |
| NCM0131A | GC Agar II- 500G |
| NCM0132A | OGYE Agar Base 500g |
| NCM0133A | Brilliant Green Agar w/ Sulfapyridine – 500g |
| NCM0134A | M-Green Yeast and Fungi Broth 500g |
| NCM0135A | Rose Bengal Chloramphenicol Agar 500g |
| NCM0136A | Rappaport-Vassiliadis Medium with Soya (RVS) (ISO) – 500g |
| NCM0137A | TSB Modified w/20mg Novobiocin & Acid Digest Casein – 500 |
| NCM0138A | Dermatophyte Test Medium (DTM) 500g |
| NCM0139A | Pancreatic Digest of Gelatin – 500g |
| NCM0140A | Lysine Iron Agar 500g |
| NCM0141A | Casman Medium Base – 500g |
| NCM0142A | LB Agar (Miller) 500g |
| NCM0143A | Tetrathionate (TT) Broth Base, Hajna 500g |
| NCM0144A | Triple Sugar Iron (TSI) Agar 500g |
| NCM0145A | Letheen Broth Base (no Tween) – 500g |
| NCM0146A | Tryptose 500g |
| NCM0147A | Sabouraud Dextrose Broth 500g |
| NCM0148A | Tryptose Phosphate Broth 500g |
| NCM0149A | mFC Agar – 500g |
| NCM0150A | Pseudomonas Isolation Agar – 500g |
| NCM0152A | Glucose OF Medium – 500g |
| NCM0153A | Phenylethanol Agar – 500g |
| NCM0154A | Schaedler Agar – 500g |
| NCM0155A | HC Agar Base – 500g |
| NCM0156A | Buffered Sodium Chloride-Peptone Broth pH 7.0 500g |
| NCM0157A | Potato Dextrose Broth – 500g |
| NCM0158A | Tryptone Glucose Extract (TGE) Agar 500g |
| NCM0159A | APT Agar – 500g |
| NCM0160A | MacConkey Agar without Crystal Violet 500g |
| NCM0161A | DNase Agar 500g |
| NCM0162A | Columbia Broth – 500g |
| NCM0163A | m-Enterococcus Agar – 500g |
| NCM0164A | Buffered Listeria Enrichment Broth Base 500g |
| NCM0165A | Bacillus cereus Agar Base (PEMBA) 500g |
| NCM0166A | Bile Esculin Azide Agar – 500g |
| NCM0167A | Sorbitol MacConkey Agar 500g |
| NCM0168A | Simmons Citrate Agar – 500g |
| NCM0170A | LB Agar, Lennox – 500g |
| NCM0171A | Universal Beer Agar – 500g |
| NCM0172A | Selenite Broth – 500g |
| NCM0173A | LB Broth, Lennox – 500g |
| NCM0174A | MacConkey Agar No. 3 – 500g |
| NCM0175A | Alkaline Saline Peptone Water (ASPW) (ISO) 500g |
| NCM0176A | Yeast & Mold Agar – 500g |
| NCM0177A | Urea Broth Base – 500g |
| NCM0178K1 | Ammonium Chloride-11g |
| NCM0178K2 | Ammonium Chloride-55g |
| NCM0179A | Minerals Modified Glutamate Agar (MMGA) – 500g |
| NCM0180A | Urea Agar Base (Christensen’s Urea Agar Base) – 500g |
| NCM0181K1 | Sodium Glutamate-130g |
| NCM0182A | Yersinia Selective Agar (Schiemann’s CIN Agar) 500g |
| NCM0183A | DRCM – 500g |
| NCM0184A | Modified Giolitti & Cantoni Broth – 500g |
| NCM0185A | Water Plate Count Agar – 500g |
| NCM0187A | Yeast Extract Dextrose Chloramphenicol Agar 500g |
| NCM0188A | R2A Broth – 500g |
| NCM0189A | Nutrient Broth No. 2 – 500g |
| NCM0190A | MRS Agar – 500g |
| NCM0191K | Ringer Solution 1/4 Strength Tablets (x100) |
| NCM0192A | Raka-Ray No. 3 Agar – 500g |
| NCM0193A | MacConkey Broth Purple – 500g |
| NCM0194A | MacConkey Agar No. 2 – 500g |
| NCM0195A | Campy Blood-Free Selective Medium (Modified CCDA)(EMEA) |
| NCM0196A | O157 Broth (Modified TSB) – 500g |
| NCM0197A | Slanetz & Bartley Agar – 500g |
| NCM0198A | Kanamycin Aesculin Azide Agar – 500g |
| NCM0199A | Fastidious Anaerobe Broth – 500g |
| NCM0200A | Baird Parker Agar Base – 500g |
| NCM0201A | LEE Broth – 500g |
| NCM0203A | Agar, Bacteriological (American Type) No. 2 – 500g |
| NCM0204A | Gelatin – 500g |
| NCM0205A | Agar, Technical – 500g |
| NCM0206A | Skim Milk, Agglomerated NFDM – 500g |
| NCM0207A | Malt Extract – 500g |
| NCM0208A | Beef Extract Powder – 500g |
| NCM0209A | Burkholderia Cepacia Selective Agar (BCSA) Complete- 500G |
| NCM0210A | Bile Salts No. 3 – 500g |
| NCM0211A | Tryptone No. 1 – 500g |
| NCM0213A | Meat Peptone No. 1 – 500g |
| NCM0214A | Agar, Bacteriological (American Type) No. 1 – 500g |
| NCM0215A | Casein Digest – 500g |
| NCM0216A | Dextrose, Anhydrous – 500g |
| NCM0217A | Yeast Extract, Ultra-Filtered-500G |
| NCM0219A | Wilkins-Chalgren Broth – 500g |
| NCM0220A | CLED Medium (Bevis Modification) – 500g |
| NCM0221A | Iron Sulphite Agar – 500g |
| NCM0222A | Nutrient Agar 1.5% – 500g |
| NCM0223A | Phosphate Buffer, pH 7.2 – 500g |
| NCM0224A | Reinforced Clostridial Agar – 500g |
| NCM0225A | Wort Agar – 500g |
| NCM0226A | Kligler Iron Agar – 500g |
| NCM0227A | Cronobacter Selective Broth – 500g |
| NCM0228A | Pancreatic Digest of Soy No. 1 – 500g |
| NCM0229A | Enzymatic Digest of Soy No. 2 – 500g |
| NCM0230A | Oxbile (Oxgall) – 500g |
| NCM0231A | Sucrose – 500g |
| NCM0232A | Meat Peptone No. 4 – 500g |
| NCM0233A | Lactose – 500g |
| NCM0235A | Cycloheximide – 500g |
| NCM0236A | Agar, Bacteriological (European Type) No.1 – 500g |
| NCM0237A | Soy Peptone – 500g |
| NCM0238A | Agar, Bacteriological (European Type) No. 2 – 500g |
| NCM0239A | Acid Hydrolysed Casein – 500g |
| NCM0241A | Glucose (Dextrose) – 500g |
| NCM0242A | Mannitol (D-Mannitol) – 500g |
| NCM0243A | Gelatin Powder – 500g |
| NCM0244A | Sodium Thiogylcollate – 500g |
| NCM0245A | Sodium Chloride (Bacteriological) – 500g |
| NCM0246A | Meat Peptone No. 3 – 500g |
| NCM0247A | Maltose, Monohydrate – 500g |
| NCM0248A | Sodium Deoxycholate- 500g |
| NCM0249A | Skim Milk Powder – 500g |
| NCM0250A | Agar No. 4 – Plant Tissue Culture Grade – 500g |
| NCM0251A | Liver Digest – 500g |
| NCM0252A | Lactalbumin Hydrolysate – 500g |
| NCM0253A | Lecithin – 500g |
| NCM0257A | Balanced Peptone No. 1 – 500g |
| NCM0258A | Mycological Peptone – 500g |
| NCM0259A | Bacteriological Peptone – 500g |
| NCM0260A | Tryptose No. 2 – 500g |
| NCM0261A | B-Pep CCT/ET 01 – 500g |
| NCM0262A | M-Pep CCT/ET 01 – 500g |
| NCM0263A | C-Pep CCT/ET 01 – 500g |
| NCM0264A | B-Pep CCT/ET 02 – 500g |
| NCM0265A | Sugar Free Agar – 500g |
| NCM0266A | m-TGE Broth – 500g |
| NCM0267A | Meat Peptone No.2 (500 g) |
| NCM0268A | Perfringens Agar (OPSP) – 500g |
| NCM0269A | Nutrient Agar (BAM) – 500g |
| NCM0270A | Buffered Peptone Water HQ (ISO) – 500g |
| NCM0271A | EC Medium, Modified w/ Novobiocin – 500 G |
| NCM0272A | Sabouraud Dextrose Agar, Emmons – 500 G |
| NCM0284A | m-HPC Agar – 500 G |
| NCM0286A | 2% Malt Extract Agar 500g |
| NCM0289A | MSRV ( Legacy 7511) – 500g |
| NCM0290A | GN Broth (Hajna) – 500 g |
| NCM0292A | Acid Digest of Casein – 500 G |
| NCM0900A | Acuferm Proteose Peptone #3 – 500 G |
| NCM0901A | Acuferm Peptone – 500 G |
| NCM0902A | Acuferm Neopeptone – 500 G |
| NCM0903A | Acutone Tryptic Soy Broth (Non-Animal TSB) – 500g |
| NCM0904A | Acutone Tryptose Phosphate Broth ( Non-Animal TPB) – 500 |
| NCM0905A | Acuferm Soytone, Advanced – 500 g |
| NCM1001A | Harlequin Tryptone Bile Glucuronide Agar (TBX) 500g |
| NCM1002A | Harlequin E. coli/Coliform Agar – 500g |
| NCM1004A | Harlequin Listeria Cromogenic Agar (Ottaviani&Agosti) – 500 |
| NCM1005A | Harlequin Cromogenic Coliform Agar – 500g |
| NCM1006A | Harlequin Chromogenic Agar for Salmonella Estrarase-500g |
| NCM1006S | Harlequin Chromogenic Agar for Salmonella Estrarase-49.9g |
| NCM1008A | Harlequin Cronobacter Isolation Agar – 500 G |
| NCM1009A | Harlequin mLGA – 500 G |
| NCM2037A | Brain Heart Infusion Broth ( legacy 7116) – 500g |
| NCM4006-2.5 | BCYE Growth Supplement (10 x 2.5L) |
| NCM4007-0.5 | GVPC Selective Supplement (10 x 500mL) |
| NCM4008-0.5 | CN Supplement (10 x 500mL) |
| NCM4009-0.5 | Ferric Ammonium Citrate (10 x 500mL) |
| NCM4010-100 | Egg Yolk Tellurite (1x100mL) |
| NCM4011-1 | Lactic Acid 10% (10 x 1mL) |
| NCM4012-10 | Potassium Tellurite 1% (10 x 10mL) |
| NCM4017-100 | Egg Yolk Emulsion 50% (1 x 100mL) |
| NCM4018-0.5 | Polymyxin B (10x 500mL) |
| NCM4018-8.0 | Polymyxin B (10 x 8L) |