Minute™粒腺體分離試劑盒/ Mitochondria Isolation Kit | 貨號MP-007 (10-40 X 10⁶ cells )

產品介紹: Minute™粒腺體分離試劑盒(Mitochondria Isolation Kit, MP-007) ，可用於哺乳動物細胞和組織；其商品具有優化的緩衝液(optimized buffers)和蛋白質提取濾芯組成(protein extraction filter cartridges)，配有2.0 ml收集管。 該試劑盒旨在從培養的哺乳動物細胞或組織中快速分離天然粒腺體蛋白(native mitochondria proteins)。 蛋白質提取濾筒 (filter cartridges)設計，使蛋白質分離程序簡單並且有效提高產率。 該程序可在不到30分鐘內完成。 與許多商業線粒體製備試劑盒不同，該試劑盒可提供多種起始細胞和分離的粒腺體。 緩沖劑不含洗滌劑和EDTA。 不需要Dounce勻漿器或組織混合器。

Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性；用於總蛋白提取，細胞分離和膜蛋白分離，只需簡單四步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。

 

Items	Quantity
Buffer A	15 ml
Buffer B	30 ml
Protein Extraction Filter Cartridges	50 units
Collection Tubes with Caps	50 units
Plastic Rods	2 units

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with Minute™ Mitochondria Isolation Kit.

1. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3, 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues, respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues, respectively.
2. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048, a nuclear envelope marker protein. abcam, Cambridge, MA).
3. Densitometry measurement of mitochondrial marker signals.

蛋白質萃取熱門商品 脂肪組織蛋白質萃取套組 | 膜蛋白萃取套組 | Endosome胞內體分離 | 凝膠蛋白質/核酸提取 |

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