LB Agar (Miller配方)培養基 | LB-Miller (10 g/L NaCl) Neogen貨號NCM0142A

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相關下載：	LB Agar (Miller配方) 培養基

特點

LB Agar (Miller配方)培養基 | 貨號NCM0142A

LB培養基（Lysogeny broth，LB）廣泛解釋爲Luria-Bertani培養基，依據其發明人貝爾塔尼（Giuseppe Bertani）的說法，這個名字來源於英語的 Lysogeny broth，即溶菌培養基。Luria-Bertani培養基是微生物學實驗中最常用的營養性培養基，用於培養大腸桿菌(E. coli i) 等細菌，Broth加入瓊脂(Agar)製成的固態培養基。加入抗生素的 LB 培養基(LB Broth) 可用於篩選大腸桿菌爲宿主的cloning。

LB Agar（Miller）是一種營養豐富的培養基，設計用於培養重組菌(recombinant strains) 的純培養物，它基於Miller描述的配方。大腸桿菌在LB培養基中生長至對數晚期 (late log phase)。一些plasmid vectors複製到high copy number並且不需要選擇性擴增。一些載體不能如此自由地複制並且需要被選擇性地擴增。可加入氯黴素(Chloramphenicol)以抑制宿主合成(host synthesis)並防止細菌染色體(bacterial chromosome)的複製。LB Agar，Miller含有10 g / L的氯化鈉，與Lennox配方中的含量不同。這允許研究人員為特定菌株選擇最佳鹽濃度。培養基可以無菌補充葡萄糖glucose或抗生素antibiotics。註明: Product was previously known as Acumedia Product: 7213 LB Agar (Miller); and LabM Product: LAB168 LB Agar (Miller)。氯化鈉的量經常隨細菌的生長需求不同而有所改變LB-Miller (10 g/L NaCl)、LB-Lennox (5 g/L NaCl)、LB-Luria (0.5 g/L NaCl)。

胰蛋白腖 (Tryptone )————————————- 10.0 g / L

酵母抽提物(Yeast Extract ) ———-5.0g / L

Sodium Chloride ————————10.0g / L

Agar—————————–15.0 g/L

Preparation培養基使用方法: 將40克培養基溶於1升純淨水中。如有必要，經常攪拌加熱以完全溶解Medium。在121℃下高壓滅菌15分鐘。等冷去後，最終7.0 ± 0.2 at 25°C, 25℃分裝於Petri dish培養皿中。

檢驗方法：檢體經系列稀釋後，以平板計數培養基培養及計數之方法。 2.1. 工作環境：工作平台須寬敞、潔淨、光線良好，操作平台光度為 100 呎燭光以上，密閉室內換氣良好，儘可能沒有灰塵及流動空氣。每 15 分鐘落菌數不得超過 15 CFU/培養皿。

Product #	Product Description
NCM0142A	LB Agar (Miller), 500 g
NCM0142B	LB Agar (Miller), 5 kg
NCM0142C	LB Agar (Miller), 10 kg
NCM0142D	LB Agar (Miller), 25 kg (European only size)
NCM0142E	LB Agar (Miller), 50 kg (US only size)

更多資料來自FDA生菌數之檢驗 (參考方法)

文獻References

European Pharmacopoeia 9th Edition (2017) 2. United States Pharmacopeia National Formulary 2017: USP 40 NF 35 3. Japanese Pharmacopeia 17th Edition (2017) 4. Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061. 5. Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl’s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.

貨號	品項
NCM0001A	Half Fraser (Demi-Fraser) Broth (ISO) 500g
NCM0002A	Tryptic Soy Agar (Soybean-Casein Digest Agar) 500g
NCM0003A	Buffered Peptone Water (BPW) 500g
NCM0004A	Tryptic Soy Broth (Soybean-Casein Digest Broth) 500g
NCM0005A	Lactose Broth 500g
NCM0007A	NFL Aseptic Validation Medium 500g
NCM0008A	Sabouraud Dextrose Agar 500g
NCM0009A	D/E Neutralizing Agar with Tween 500g
NCM0010A	Plate Count Agar (Standard Methods) 500g
NCM0011A	Tryptic Soy Agar with Lecithin and Tween 80 500g
NCM0012A	UVM Broth 500g
NCM0013A	Columbia Agar 500g
NCM0014A	Fastidious Anaerobe Agar 500g
NCM0015A	Buffered Peptone Water (BPW) (ISO) 500g
NCM0016A	Brain Heart Infusion Broth 500g
NCM0017A	MacConkey Agar 500g
NCM0018A	Potato Dextrose Agar 500g
NCM0019A	Tryptone Soy Broth 500g
NCM0020A	Tryptone Soy Agar 500g
NCM0021A	Xylose Lysine Deoxycholate (XLD) Agar (ISO) 500g
NCM0022A	Violet Red Bile Glucose Agar (VRBGA) 500g
NCM0023A	Mueller Hinton Agar II 500g
NCM0024A	Baird-Parker Agar 500g
NCM0025A	Violet Red Bile Agar 500g
NCM0026A	Tryptic Soy Broth, Modified with Acid Digest of Casein 500g
NCM0027A	Xylose Lysine Deoxycholate (XLD) Agar 500g
NCM0028A	Corn Meal Agar 500g
NCM0029A	DRBC Agar (BAM) 500g
NCM0030A	Lauryl Sulfate Broth 500g
NCM0031A	Columbia Blood Agar Base 500g
NCM0032A	Lauryl Tryptose (LST) Broth 500g
NCM0033A	Nutrient Agar 500g
NCM0034A	Cystine Lactose Electrolyte Deficient (CLED) Agar 500g
NCM0035A	Lactobacilli MRS Agar 500g
NCM0037A	BCYE Agar (Legionella Isolation Medium) 500g
NCM0038A	Columbia Agar Base 500g
NCM0039A	Membrane Lauryl Sulphate Broth (500 g)
NCM0040A	Blood Agar Base No. 2 500g
NCM0041A	Violet Red Bile Glucose Agar (ISO) 500g
NCM0042A	Campy Blood-Free Selective Medium (Modified CCDA) – 500g
NCM0043A	Middlebrook 7H11 Agar 500g
NCM0044A	Universal Preenrichment Broth 500g
NCM0045A	GC Agar 500g
NCM0046A	Salmonella Shigella (SS) Agar 500g
NCM0047A	D/E Neutralizing Broth with Tween 500g
NCM0048A	Brilliant Green Bile 2% Broth – 500g
NCM0048A	Brilliant Green Bile 2% Broth – 500g
NCM0049A	PALCAM Broth – 500g
NCM0050A	Fraser Broth 500g
NCM0051A	Buffered Listeria Enrichment Broth 500g
NCM0052A	Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar 500g
NCM0053A	Acutone PPLO Broth w/o Crystal Violet – 500g
NCM0054A	Orange Serum Agar 500g
NCM0055A	Listeria Enrichment Broth 500g
NCM0056A	Oxford Listeria Agar 500g
NCM0058A	Brilliant Green Agar (Modified) 500g
NCM0059A	YM Broth – 500g
NCM0060A	MacConkey Broth 500g
NCM0061A	Todd Hewitt Broth 500g
NCM0062A	Bacillus cereus MYP Agar – 500g
NCM0063A	Tryptic Soy Broth, Modified with Novobiocin 500g
NCM0064A	Violet Red Bile Agar with MUG (VBRA-MUG) 500g
NCM0065A	EC Broth 500g
NCM0066A	Fraser Broth Base 500g
NCM0067A	Modified Semi-solid Rappaport-Vassiladis (MSRV) Agar 500g
NCM0068A	Sabouraud Dextrose Agar with Chloramphenicol 500g
NCM0069A	Yeast Extract Agar 500g
NCM0070A	Eugon Broth 500g
NCM0071A	Lauryl Sulfate Broth w/ MUG – 500g
NCM0072A	MacConkey Agar without Crystal Violet and Salt 500g
NCM0073A	Dextrose Tryptone Broth – 500g
NCM0074A	KF Streptococcus Agar – 500g
NCM0075A	Blood Agar Base 500g
NCM0076A	R2A Agar 500g
NCM0077A	Perfrigens Agar Base (TSC) 500g
NCM0078A	Mannitol Salt Agar – 500g
NCM0079A	Lactobacilli MRS Broth 500g
NCM0080A	Brain Heart Infusion Agar – 500g
NCM0081A	Dichloran Glycerol (DG-18) Agar Base 500g
NCM0082A	DRBC Agar (ISO) 500g
NCM0083A	Pseudomonas Agar Base 500g
NCM0084A	Modified Buffered Peptone Water with Pyruvate – 500g
NCM0085A	Maximum Recovery Diluent (Tryptone Salt Broth) 500g
NCM0086A	Bismuth Sulfite Agar 500g
NCM0087A	Tryptose Broth – 500g
NCM0088A	LB Broth (Miller) 500g
NCM0089A	Violet Red Bile Agar (VRBA) (ISO) – 500g
NCM0090A	Brucella Agar – 500g
NCM0091A	TAT Broth – 500g
NCM0092A	Tetrathionate Broth Base 500g
NCM0093A	Malt Extract Agar 500g
NCM0094A	Campylobacter Enrichment Broth (Bolton Broth) 500g
NCM0095A	Sabouraud Dextrose Agar (with Lecithin & Tween) – 500g
NCM0096A	Peptone Water 500g
NCM0098A	Tryptic Soy Blood Agar Base No. 2 – 500g
NCM0099A	Campy Cefex Agar – 500g
NCM0101A	TSB, Modified w/ 8mg/L Novobiocin & ADC – 500g
NCM0102A	Reinforced Clostridial Medium 500g
NCM0103A	Rappaport Vassiliadis Salmonella Enrichment Broth 500g
NCM0104A	Letheen Broth with Tween, Modified – 500g
NCM0105A	Eosin Methylene Blue (EMB) Agar Levine 500g
NCM0106A	Wilkins-Chalgren Agar – 500g
NCM0107A	EC Medium w/ MUG – 500g
NCM0108A	Fluid Thioglycollate Medium 500g
NCM0109A	Cetrimide Agar 500g
NCM0110A	Nutrient Broth 500g
NCM0111A	Palcam Agar Base 500g
NCM0112A	DC Medium with BCIG – 500g
NCM0113A	PPLO Broth Base without Crystal Violet 500g
NCM0115A	Columbia CNA Agar – 500g
NCM0116A	Letheen Broth (with Tween) – 500g
NCM0117A	Bile Esculin Agar 500g
NCM0118A	WL Nutrient Agar 500g
NCM0119A	Milk Plate Count Agar 500g
NCM0120A	Casein Peptone Type I – 500g
NCM0121A	D/E Neutralizing Broth without Tween 500g
NCM0122A	Presence Absence Broth – 500g
NCM0123A	m-Endo Agar – 500g
NCM0124A	A-1 Medium – 500g
NCM0125A	M Broth – 500g
NCM0126A	Muller-Kauffmann Tetrathionate-Novobiocin (MKTTn) Broth 5
NCM0127A	Dextrose Tryptone Agar 500g
NCM0128A	Clostridium Diffcile Agar Base (Brazier’s) 500g
NCM0129A	Letheen Broth Base Modified without Tween – 500g
NCM0130A	Letheen Agar Base Modified without Tween – 500g
NCM0131A	GC Agar II- 500G
NCM0132A	OGYE Agar Base 500g
NCM0133A	Brilliant Green Agar w/ Sulfapyridine – 500g
NCM0134A	M-Green Yeast and Fungi Broth 500g
NCM0135A	Rose Bengal Chloramphenicol Agar 500g
NCM0136A	Rappaport-Vassiliadis Medium with Soya (RVS) (ISO) – 500g
NCM0137A	TSB Modified w/20mg Novobiocin & Acid Digest Casein – 500
NCM0138A	Dermatophyte Test Medium (DTM) 500g
NCM0139A	Pancreatic Digest of Gelatin – 500g
NCM0140A	Lysine Iron Agar 500g
NCM0141A	Casman Medium Base – 500g
NCM0142A	LB Agar (Miller) 500g
NCM0143A	Tetrathionate (TT) Broth Base, Hajna 500g
NCM0144A	Triple Sugar Iron (TSI) Agar 500g
NCM0145A	Letheen Broth Base (no Tween) – 500g
NCM0146A	Tryptose 500g
NCM0147A	Sabouraud Dextrose Broth 500g
NCM0148A	Tryptose Phosphate Broth 500g
NCM0149A	mFC Agar – 500g
NCM0150A	Pseudomonas Isolation Agar – 500g
NCM0152A	Glucose OF Medium – 500g
NCM0153A	Phenylethanol Agar – 500g
NCM0154A	Schaedler Agar – 500g
NCM0155A	HC Agar Base – 500g
NCM0156A	Buffered Sodium Chloride-Peptone Broth pH 7.0 500g
NCM0157A	Potato Dextrose Broth – 500g
NCM0158A	Tryptone Glucose Extract (TGE) Agar 500g
NCM0159A	APT Agar – 500g
NCM0160A	MacConkey Agar without Crystal Violet 500g
NCM0161A	DNase Agar 500g
NCM0162A	Columbia Broth – 500g
NCM0163A	m-Enterococcus Agar – 500g
NCM0164A	Buffered Listeria Enrichment Broth Base 500g
NCM0165A	Bacillus cereus Agar Base (PEMBA) 500g
NCM0166A	Bile Esculin Azide Agar – 500g
NCM0167A	Sorbitol MacConkey Agar 500g
NCM0168A	Simmons Citrate Agar – 500g
NCM0170A	LB Agar, Lennox – 500g
NCM0171A	Universal Beer Agar – 500g
NCM0172A	Selenite Broth – 500g
NCM0173A	LB Broth, Lennox – 500g
NCM0174A	MacConkey Agar No. 3 – 500g
NCM0175A	Alkaline Saline Peptone Water (ASPW) (ISO) 500g
NCM0176A	Yeast & Mold Agar – 500g
NCM0177A	Urea Broth Base – 500g
NCM0178K1	Ammonium Chloride-11g
NCM0178K2	Ammonium Chloride-55g
NCM0179A	Minerals Modified Glutamate Agar (MMGA) – 500g
NCM0180A	Urea Agar Base (Christensen’s Urea Agar Base) – 500g
NCM0181K1	Sodium Glutamate-130g
NCM0182A	Yersinia Selective Agar (Schiemann’s CIN Agar) 500g
NCM0183A	DRCM – 500g
NCM0184A	Modified Giolitti & Cantoni Broth – 500g
NCM0185A	Water Plate Count Agar – 500g
NCM0187A	Yeast Extract Dextrose Chloramphenicol Agar 500g
NCM0188A	R2A Broth – 500g
NCM0189A	Nutrient Broth No. 2 – 500g
NCM0190A	MRS Agar – 500g
NCM0191K	Ringer Solution 1/4 Strength Tablets (x100)
NCM0192A	Raka-Ray No. 3 Agar – 500g
NCM0193A	MacConkey Broth Purple – 500g
NCM0194A	MacConkey Agar No. 2 – 500g
NCM0195A	Campy Blood-Free Selective Medium (Modified CCDA)(EMEA)
NCM0196A	O157 Broth (Modified TSB) – 500g
NCM0197A	Slanetz & Bartley Agar – 500g
NCM0198A	Kanamycin Aesculin Azide Agar – 500g
NCM0199A	Fastidious Anaerobe Broth – 500g
NCM0200A	Baird Parker Agar Base – 500g
NCM0201A	LEE Broth – 500g
NCM0203A	Agar, Bacteriological (American Type) No. 2 – 500g
NCM0204A	Gelatin – 500g
NCM0205A	Agar, Technical – 500g
NCM0206A	Skim Milk, Agglomerated NFDM – 500g
NCM0207A	Malt Extract – 500g
NCM0208A	Beef Extract Powder – 500g
NCM0209A	Burkholderia Cepacia Selective Agar (BCSA) Complete- 500G
NCM0210A	Bile Salts No. 3 – 500g
NCM0211A	Tryptone No. 1 – 500g
NCM0213A	Meat Peptone No. 1 – 500g
NCM0214A	Agar, Bacteriological (American Type) No. 1 – 500g
NCM0215A	Casein Digest – 500g
NCM0216A	Dextrose, Anhydrous – 500g
NCM0217A	Yeast Extract, Ultra-Filtered-500G
NCM0219A	Wilkins-Chalgren Broth – 500g
NCM0220A	CLED Medium (Bevis Modification) – 500g
NCM0221A	Iron Sulphite Agar – 500g
NCM0222A	Nutrient Agar 1.5% – 500g
NCM0223A	Phosphate Buffer, pH 7.2 – 500g
NCM0224A	Reinforced Clostridial Agar – 500g
NCM0225A	Wort Agar – 500g
NCM0226A	Kligler Iron Agar – 500g
NCM0227A	Cronobacter Selective Broth – 500g
NCM0228A	Pancreatic Digest of Soy No. 1 – 500g
NCM0229A	Enzymatic Digest of Soy No. 2 – 500g
NCM0230A	Oxbile (Oxgall) – 500g
NCM0231A	Sucrose – 500g
NCM0232A	Meat Peptone No. 4 – 500g
NCM0233A	Lactose – 500g
NCM0235A	Cycloheximide – 500g
NCM0236A	Agar, Bacteriological (European Type) No.1 – 500g
NCM0237A	Soy Peptone – 500g
NCM0238A	Agar, Bacteriological (European Type) No. 2 – 500g
NCM0239A	Acid Hydrolysed Casein – 500g
NCM0241A	Glucose (Dextrose) – 500g
NCM0242A	Mannitol (D-Mannitol) – 500g
NCM0243A	Gelatin Powder – 500g
NCM0244A	Sodium Thiogylcollate – 500g
NCM0245A	Sodium Chloride (Bacteriological) – 500g
NCM0246A	Meat Peptone No. 3 – 500g
NCM0247A	Maltose, Monohydrate – 500g
NCM0248A	Sodium Deoxycholate- 500g
NCM0249A	Skim Milk Powder – 500g
NCM0250A	Agar No. 4 – Plant Tissue Culture Grade – 500g
NCM0251A	Liver Digest – 500g
NCM0252A	Lactalbumin Hydrolysate – 500g
NCM0253A	Lecithin – 500g
NCM0257A	Balanced Peptone No. 1 – 500g
NCM0258A	Mycological Peptone – 500g
NCM0259A	Bacteriological Peptone – 500g
NCM0260A	Tryptose No. 2 – 500g
NCM0261A	B-Pep CCT/ET 01 – 500g
NCM0262A	M-Pep CCT/ET 01 – 500g
NCM0263A	C-Pep CCT/ET 01 – 500g
NCM0264A	B-Pep CCT/ET 02 – 500g
NCM0265A	Sugar Free Agar – 500g
NCM0266A	m-TGE Broth – 500g
NCM0267A	Meat Peptone No.2 (500 g)
NCM0268A	Perfringens Agar (OPSP) – 500g
NCM0269A	Nutrient Agar (BAM) – 500g
NCM0270A	Buffered Peptone Water HQ (ISO) – 500g
NCM0271A	EC Medium, Modified w/ Novobiocin – 500 G
NCM0272A	Sabouraud Dextrose Agar, Emmons – 500 G
NCM0284A	m-HPC Agar – 500 G
NCM0286A	2% Malt Extract Agar 500g
NCM0289A	MSRV ( Legacy 7511) – 500g
NCM0290A	GN Broth (Hajna) – 500 g
NCM0292A	Acid Digest of Casein – 500 G
NCM0900A	Acuferm Proteose Peptone #3 – 500 G
NCM0901A	Acuferm Peptone – 500 G
NCM0902A	Acuferm Neopeptone – 500 G
NCM0903A	Acutone Tryptic Soy Broth (Non-Animal TSB) – 500g
NCM0904A	Acutone Tryptose Phosphate Broth ( Non-Animal TPB) – 500
NCM0905A	Acuferm Soytone, Advanced – 500 g
NCM1001A	Harlequin Tryptone Bile Glucuronide Agar (TBX) 500g
NCM1002A	Harlequin E. coli/Coliform Agar – 500g
NCM1004A	Harlequin Listeria Cromogenic Agar (Ottaviani&Agosti) – 500
NCM1005A	Harlequin Cromogenic Coliform Agar – 500g
NCM1006A	Harlequin Chromogenic Agar for Salmonella Estrarase-500g
NCM1006S	Harlequin Chromogenic Agar for Salmonella Estrarase-49.9g
NCM1008A	Harlequin Cronobacter Isolation Agar – 500 G
NCM1009A	Harlequin mLGA – 500 G
NCM2037A	Brain Heart Infusion Broth ( legacy 7116) – 500g
NCM4006-2.5	BCYE Growth Supplement (10 x 2.5L)
NCM4007-0.5	GVPC Selective Supplement (10 x 500mL)
NCM4008-0.5	CN Supplement (10 x 500mL)
NCM4009-0.5	Ferric Ammonium Citrate (10 x 500mL)
NCM4010-100	Egg Yolk Tellurite (1x100mL)
NCM4011-1	Lactic Acid 10% (10 x 1mL)
NCM4012-10	Potassium Tellurite 1% (10 x 10mL)
NCM4017-100	Egg Yolk Emulsion 50% (1 x 100mL)
NCM4018-0.5	Polymyxin B (10x 500mL)
NCM4018-8.0	Polymyxin B (10 x 8L)

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