肉毒桿菌毒素B檢測 | Botulinum Toxin B ELISA Kit Tetracore貨號TC-4037-002
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肉毒桿菌毒素B檢測 | Botulinum Toxin B ELISA Kit Tetracore貨號TC-4037-002
To detect Botulinum toxin B in unknown samples using an indirect capture ELISA with monoclonal as capture and rabbit polyclonal as detector.
肉毒桿菌毒素（BTX）是由肉毒桿菌和相關物種產生的神經毒性蛋白質。有八種類型的肉毒桿菌毒素，名為A-H型。Tetracore提供肉毒桿菌毒素A（Botulinum Toxin A）、肉毒桿菌毒素A&B（Botulinum Toxin A&B）、肉毒桿菌毒素C（Botulinum Toxin C）、肉毒桿菌毒素E（Botulinum Toxin E）、肉毒桿菌毒素F（Botulinum Toxin F）等ELISA檢測試劑盒，可偵測環境中的肉毒桿菌。產品具有(+/-) 的ELISA會有positive和negative的capture antibody行行交錯，negative可直接對照上行的positive結果，在混合物的sample中可得到較佳的結果。【注意事項】本類套組不含標準品（需自備），Not for diagnostic use of clinical samples。
產品描述：肉毒桿菌毒素B (Botulinum Toxin B) 抗原檢測套組，應用抗體與抗原之間具有高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。產品設計為使用單株肉毒桿菌毒素B (anti-Bot Toxin B Monoclonal)作為Capture捕獲抗體，固定於96well，經blocking後，加入測試樣品/標準品/ ELISA Dilution/Blocking Buffer; 37度C反應1小時，以PBST清洗4次後。再加入第二個兔抗肉毒桿菌毒素B (Rabbit anti-bot tox Polyclonal)多株抗體作為Detect偵測抗體， 經若待測樣品含有肉毒桿菌毒素B，經由具標誌HRP之抗免二級抗體 (Goat anti-Rabbit IgG – HRP)結合，加入ABTS反應後，使用ELISA儀器在ODs 405 and 410 nm之間可測得讀值。 注意事項: pre-coated（+/-）肉毒桿菌毒素B BioThreat Alert ELISA試劑盒（TC-4037-002）無須再進行陽性捕獲抗體(Positive Capture Antibody)及陰性捕獲抗體 (Negative Capture Antibody)之coated動作，試劑中亦不包括陽性捕獲抗體(Positive Capture Antibody)及陰性捕獲抗體 (Negative Capture Antibody)。此產品不包括肉毒桿菌毒素B之標準品。需經由儀器測量讀值 ELISA儀器設備請按此
套組內不包括之設備及試劑
ELISA儀器 (optional)、ELISA Washer (optional)、Finnpipette F1 微量分注器 (單爪/8/12) 、20ul, 200ul, 1000ul Tips 微量吸管、De-ionized or filtered water、37oC 培養箱、肉毒桿菌毒素B (Botulinum toxin B)標準品、電磁加熱攪拌器 (Stir plate)、可攜式生化廢液抽吸器、PBS (Phosphate Buffered Saline) packet, yielding 1 liter 0.01 M phosphate buffered、Tween-20、Aluminum foil
套組內之試劑內容 (Storage at 4 oC)
兩個96孔平底ELISA板 (密封鋁箔袋)
Positive Capture Antibody (Mab anti-Bot Toxin B), 1.5 mg/mL, Lot T291007-01*
Negative Capture Antibody (Neg. Mab 114-14G2), 1.1mg/mL, Lot T260209-01; *
Detector Antibody (rabbit anti-bot tox), 1.0 mg/mL, Lot T140206-01
Conjugate Antibody (Goat anti-Rabbit IgG – HRP), 0.4 mg/mL
Dry skim milk
ABTS peroxidase Substrate (1-component)
Buffer配製
PBS Coating Buffer：1升去離子水或過濾水中加入一包PBS (0.01 M phosphate buffered) 。使用電磁加熱攪拌器的磁力攪拌棒混合溶液直至溶液溶解。以0.22μm過濾。在4°C下儲存長達一個月。PBST (Phosphate Buffered Saline with Tween-20)：向800mL PBS包衣緩衝液中加入0.8mL Tween-20，使溶液混合直至溶解。在4°C下儲存長達一個月。ELISA稀釋/封閉緩衝液 (ELISA Dilution/Blocking Buffer)：對於每個使用的平板，將5克乾脫脂乳加入90mL PBST中。使用攪拌棒和攪拌板使混合物進入溶液。使用氫氧化鈉或等效物將pH調節至7.4。添加PBST使總體積達到100 mL。在4°C下儲存最多3天，或在-20°C下等分儲存最多一個月。注意：對於長期儲存，可以在緩衝液中加入0.001％ thimerosal（Sigma T5125）。，製備1％thimerosal在去離子水或過濾水中的溶液，然後每1升緩衝液加入1mL該混合物，產生0.001％溶液。
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肉毒桿菌毒素A檢測（Botulinum Toxin A） |
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| TC-4046-002 | Botulinum Toxin A Complex, non-coated (+/-) | Two 96 well plates/ kit |
| TC-4045-001 | Botulinum Toxin A Complex, non-coated (+ only) | One 96 well plates/ kit |
| TC-4048-002 | Botulinum Toxin A Complex, pre-coated (+/-) | Two 96 well plates/ kit |
| TC-4047-001 | Botulinum Toxin A Complex, pre-coated (+ only) | One 96 well plates/ kit |
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肉毒桿菌毒素A&B檢測（Botulinum Toxin A&B） |
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| TC-4009-002 | Botulinum Toxin A&B, non-coated (+/-) | Two 96 well plates/ kit |
| TC-4010-002 | Botulinum Toxin A&B, pre-coated (+/-) | Two 96 well plates/ kit |
| TC-4011-001 | Botulinum Toxin A&B, non-coated (+ only) | One 96 well plate/ kit |
| TC-4012-001 | Botulinum Toxin A&B, pre-coated (+ only ) | One 96 well plate/ kit |
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肉毒桿菌毒素B檢測（Botulinum Toxin B） |
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| TC-4039-002 | Botulinum Toxin B, non-coated (+/-) | Two 96 well plates/ kit |
| TC-4037-002 | Botulinum Toxin B, pre-coated (+/-) | Two 96 well plates/ kit |
| TC-4040-001 | Botulinum Toxin B, non-coated (+ only) | One 96 well plate/ kit |
| TC-4038-001 | Botulinum Toxin B, pre-coated (+ only ) | One 96 well plate/ kit |
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肉毒桿菌毒素C檢測（Botulinum Toxin C） |
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| TC-4058-002 | Botulinum Toxin C, non-coated (+/-) | Two 96 well plates/ kit |
| TC-4057-001 | Botulinum Toxin C, non-coated (+ only) | One 96 well plates/ kit |
| TC-4060-002 | Botulinum Toxin C, pre-coated (+/-) | Two 96 well plates/ kit |
| TC-4059-001 | Botulinum Toxin C, pre-coated (+ only) | One 96 well plates/ kit |
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肉毒桿菌毒素E檢測（Botulinum Toxin E） |
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| TC-4053-001 | Botulinum Toxin E, non-coated (+ only) | One 96 well plates/ kit |
| TC-4054-002 | Botulinum Toxin E, non-coated (+/-) | Two 96 well plates/ kit |
| TC-4055-001 | Botulinum Toxin E, pre-coated (+ only) | One 96 well plates/ kit |
| TC-4056-002 | Botulinum Toxin E, pre-coated (+/-) | Two 96 well plates/ kit |
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肉毒桿菌毒素F檢測（Botulinum Toxin F） |
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| TC-4050-002 | Botulinum Toxin F, non-coated (+/-) | Two 96 well plates/ kit |
| TC-4052-002 | Botulinum Toxin F, pre-coated (+/-) | Two 96 well plates/ kit |
| TC-4049-001 | Botulinum Toxin F, non-coated (+ only) | One 96 well plates/ kit |
| TC-4051-001 | Botulinum Toxin F, pre-coated (+ only) | One 96 well plates/ kit |