Recombinant Myostatin (MSTN)重組蛋白 |貨號RPB653Mu01
附Protein marker 或 positive control
Active Myostatin (MSTN)活性重組蛋白 |貨號APB653Mu01
產品介紹: Cloud clone提供原核及真核生物表現之重組蛋白系列商品；其中Active Glial Cell Line Derived Neurotrophic Factor (GDNF)活性重組蛋白，為活性蛋白，Organism Species Mus musculus (Mouse)。Isoelectric Point6.5，純度> 90%，提供凍乾型式包裝。可應用於Cell culture; Activity Assays等不同研究。
蛋白質保存型式
蛋白質溶於20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.為冷凍乾燥包裝。使用前Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL避免vortex. 短期保存Store at 2-8°C for one month.分裝-80°C長期12 個月。請避免反覆冷凍解凍。
MSTN與FSTL3的結合活性。
肌肉生長抑制素（MSTN）也稱為生長分化因子8（GDF-8），一種由肌細胞產生和釋放的蛋白質。它抑制肌肉生成，包括肌細胞生長和分化。肌肉生長抑制素是分泌的生長分化因子，是TGFβ蛋白家族的成員。此外，Follistatin Like Protein 3（FSTL3）已被鑑定為MSTN的相互作用，因此進行結合ELISA測定以檢測重組小鼠MSTN與重組小鼠FSTL3的相互作用。簡言之，將MSTN在含有0.01％BSA（pH7.4）的PBS中連續稀釋。然後將100μL的重複樣品轉移到FSTL3包被的微量滴定孔中，並在37℃下孵育2小時。用PBST洗滌孔並與抗MSTN pAb一起incubate1小時，然後吸出並洗滌3次。與HRP標記的第二抗體incubate後，吸出孔並洗滌3次。加入底物溶液，將孔在37℃下孵育15-25分鐘。最後，向孔中加入50μL終止溶液，立即讀取450nm。 MSTN和FSTL3的結合活性如圖1所示，該作用呈劑量依賴性。
Active Myostatin (MSTN)活性重組蛋白相關之抗體 | 蛋白質 產品
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB653Mu02 | Recombinant Myostatin (MSTN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB653Mu01 | Active Myostatin (MSTN) | Cell culture; Activity Assays. |
| RPB653Mu01 | Recombinant Myostatin (MSTN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB653Mu21 | OVA Conjugated Myostatin (MSTN) | Immunogen; SDS-PAGE; WB. |
| PAB653Mu08 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
| PAB653Mu02 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
| PAB653Mu01 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
| LAB653Mu71 | Biotin-Linked Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC. |
| SEB653Mu | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB653Mu | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| KSB653Mu11 | ELISA Kit DIY Materials for Myostatin (MSTN) | Main materials for “Do It (ELISA Kit) Yourself”. |