天冬氨酸轉氨酶(AST)活性測定試劑盒 | AST/SGOT Activity Colorimetric Assay Kit貨號K753-100

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天冬氨酸轉氨酶(AST)活性測定試劑盒 | 貨號K753-100
Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit
天冬氨酸轉氨酶(AST),也被稱為谷氨酸 – 草酰乙酸轉氨酶(GOT)是一種轉氨酶(EC 2.6.1.1),類似於肝臟特異性丙氨酸轉氨酶(ALT)。儘管臨床上通常將其作為診斷性肝功能試驗的一部分,但是AST具有更廣泛的臨床應用,因為它也可能在影響其它器官的疾病如心肌梗塞的心臟或肌肉中升高,在急性胰腺炎,急性溶血性貧血,嚴重燒傷,急性腎病,肌肉骨骼疾病和外傷。它催化反應:天冬氨酸+ K-酮戊二酸⇌草酰乙酸+谷氨酸在診斷上,它幾乎總是以單位/升(U / L)來衡量。在BioVision的AST分析試劑盒中,氨基從天冬氨酸轉移到β-酮戊二酸。這種可逆轉氨反應的產物是草酰乙酸和谷氨酸。在將幾乎無色的探針伴隨轉化為顏色(λmax= 450nm)的反應中檢測到谷氨酸鹽。該試劑盒提供了一種快速,簡單,靈敏和可靠的檢測方法,適用於AST的高通量活性檢測,檢測限為每孔10 mU。
ELISA儀器設備請按此 (標配405、450、492、630 nm濾片/最高可擴充至7濾片)(k753 λ= 450 nm)
| Cat # +Size | K753-100 |
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Mammalian |
| Applications | This assay detects AST/ SGOT with a detection limit of 10 mU per well. |
| Features & Benefits | • Simple procedure • Fast, reliable and convenient • Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses. |
| Kit Components | • AST Assay Buffer • AST Enzyme Mix • Developer • AST Substrate • Glutamate Standard (0.1M) • AST Positive Control |
白蛋白(BCG)檢測 | (AST)活性測定試劑 | ALT/SGPT)活性比色螢光測定試劑 | Ammonia測定試劑 | 膽紅素分析試劑盒
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