HDAC組織蛋白去乙醯酶活性分析套組 | HDAC Activity Colorimetric Assay Kit貨號K331-100

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特點

組織蛋白去乙醯酶抑制劑及藥物篩選分析研究

組織蛋白去乙醯酶，是促使組織蛋白Histone去乙醯化的一種酵素。當組蛋白去乙醯酶不正常表現活性，造成其作用的蛋白質乙醯化程度失去平衡，會導致許多疾病如癌症、神經退化性疾病等。目前，組蛋白去乙醯化酶的抑制劑已做為抗腫瘤細胞增生、促成細胞凋亡、促使腫瘤細胞分化、並抑制血管新生作用。因此HDACs 為具有潛力的癌症治療標靶。Biovision提供一系列組織蛋白去乙醯酶活性分析(HDAC Activity assay)、組織蛋白去乙醯酶抑制劑快篩套組 (HDAC inhibitor screening assay kit)、及組織蛋白去乙醯酶抑制劑 (histone deacetylase inhibitors, HDACIs )等系列商品。

HDAC組織蛋白去乙醯酶活性分析套組 | 貨號K331-100

HDAC Activity Colorimetric Assay Kit, Two step

產品描述：組蛋白脫乙酰酶（HDAC）的抑制涉及調節轉錄和誘導癌細胞凋亡或分化。然而，由於缺乏用於分析HDAC活性的方便的工具，篩選HDAC抑制化合物在過去被證明是困難的。新的比色HDAC活性測定試劑盒提供了一種快速便捷的呈色法，可以消除傳統測定中使用的放射性，萃取或色譜繁瑣之步驟。新方法只需要兩個簡單的步驟，兩個都在相同的微量滴定板上進行。首先，將包含乙醯化賴氨酸側鏈的HDAC比色底物與含有HDAC活性的樣品（例如HeLa核提取物或您自己的樣品）一起孵育。底物的脫乙醯化對底物敏感，因此在第二步中，與賴氨酸顯影劑相結合產生髮色團。可以使用ELISA板讀數器或分光光度計輕鬆分析髮色團。該測定非常適合於高通量篩選應用。 HDAC抑制劑和抗體也可單獨使用。

Cat # +Size	K331-100
Size	100 assays
Detection Method	Absorbance (400 or 405 nm)
Species Reactivity	Mammalian
Applications	Well suited for high throughput applications. HDAC inhibitors and substrates and related antibodies are also available separately.
Features & Benefits	• Simple two-step procedure; takes around than 1 hour • Fast and convenient • The assay method eliminates radioactivity/extractions/ and/or chromatography as used in the traditional assays.
Kit Components	• HDAC Substrate [Ac-Lys(Ac)-pNA, 10 mM] • 10X HDAC Assay Buffer • Lysine Developer • HDAC Inhibitor (Trichostatin A, 1 mM) • HeLa Nuclear Extract (5 mg/ml) • Deacetylated Standard (Ac-Lys-pNA, 10 mM)

引用文獻

Tzeng, Haiyue et al. (2016) Antioxidant-Rich Extract from Plantaginis Semen Ameliorates Diabetic Retinal Injury in a Streptozotocin-Induced Diabetic Rat Model, Nutrients. 2016 Sep 18;8(9).

Gurav et al., Slc5a8, a Na+-coupled high-affinity transporter for short-chain fatty acids, is a conditional tumour suppressor in colon that protects against colitis and colon cancer under low-fibre dietary conditions. Biochem. J., Jul 2015; 469: 267 – 278.

Yang et al., PARP-1 Mediates LPS-Induced HMGB1 Release by Macrophages through Regulation of HMGB1 Acetylation.J. Immunol., Dec 2014; 193: 6114 – 6123.

Zhang et al., Specific inhibition of HDAC4 in cardiac progenitor cells enhances myocardial repairs. Am J Physiol Cell Physiol, Aug 2014; 307: C358 – C372.

Aslan et al., Histone deacetylase 6-mediated deacetylation of α-tubulin coordinates cytoskeletal and signaling events during platelet activation. Am J Physiol Cell Physiol, Dec 2013; 305: C1230 – C1239.

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