【血清膽固醇代謝檢測】總膽固醇/游離甘油/LDL/HDL分析-Abcam (Biovision)

Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II

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膽固醇 (Cholesterol)是所有動物生命中必不可少的分子。它涉及正常發育(normal development)和疾病(diseases)。膽固醇/膽固醇酯定量試劑盒 (Cholesterol/Cholesteryl Ester Quantitation Kit) 提供了一種使用比色法靈敏定量游離膽固醇(free cholestero)，膽固醇酯(cholesteryl esters)或兩者的簡單方法。血液中的大多數膽固醇是膽固醇酯(cholesteryl esters)的形式。這些酯(esters )可在適當條件下水解成游離膽固醇(free cholesterol)。在該測定中，游離膽固醇(free cholesterol)被膽固醇脫氫酶(cholesterol dehydrogenase)氧化以產生NADH，其與敏感探針反應，導致450nm處的強吸收。該測定可以檢測游離膽固醇(free cholesterol)或總膽固醇(total cholesterol)，這取決於酯酶是否用於水解存在的膽固醇酯。膽固醇酯 ( Cholesteryl ester )可以通過從總膽固醇（膽固醇加膽固醇酯）中減去游離膽固醇(free cholesterol )的值來確定。該試劑盒中的探針更穩定，靈敏且特異。該測定可以顯著地耐受來自各種樣品的干擾。

Cat # +Size	K623-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Species Reactivity	Mammalian
Applications	N/A
Features & Benefits	• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains the necessary reagents for measurement of cholesterol in various biological samples
Kit Components	• Cholesterol Assay Buffer • Substrate Mix (lyophilized) • Enzyme Mix (lyophilized) • Esterase (lyophilized) • Cholesterol Standard (2 µg/µl)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

引用文獻

Sundaram, M. et al., (2017) he apolipoprotein C-III (Gln38Lys) variant associated with human hypertriglyceridemia is a gain-of-function mutation, Journal of Lipid Research, Sep.2017, jlr-M077313

Lin, NN et al., (2017) Bacillus Subtilis-Fermented Red Bean (Red Bean Natto) Reduces Hyperlipidemia Levels in Hamsters Fed an Atherogenic Diet, J Food Biochemistry, 2017, 41(1): online

Liu et al., Apoc2 loss-of-function zebrafish mutant as a genetic model of hyperlipidemia. Dis. Model. Mech., Aug 2015; 8: 989 – 998.

Xu et al., Rutaecarpine suppresses atherosclerosis in ApoE–/– mice through upregulating ABCA1 and SR-BI within RCT. J. Lipid Res., Aug 2014; 55: 1634 – 1647.

Watanabe et al., Lipocalin 2 binds to membrane phosphatidylethanolamine to induce lipid raft movement in a PKA-dependent manner and modulates sperm maturation. Development, May 2014; 141: 2157 – 2164.

(不同樣品來源) 游離甘油比色/螢光檢測試劑盒 | 貨號K630-100

Free Glycerol Colorimetric/Fluorometric Assay Kit

產品描述：甘油廣泛用於食品，飲料，溶劑，藥物和化妝品等。人們對用於研究和開發的甘油的定量具有廣泛的興趣。 BioVision的甘油分析試劑盒提供靈敏，簡便的分析方法，可測量各種樣品中的游離甘油濃度。在該測定中，甘油被酶促氧化以產生與探針反應以產生顏色（λ= 570nm）和螢光（Ex / Em = 535 / 587nm）的產物。該試劑盒可以檢測各種樣品中50pmol-10nmol（或1~10000μM範圍）的甘油。

Cat # +Size	K630-100
Size	100 assays
Detection Method	Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Applications	The kit can detect 50 pmol-10 nmol (or 1~10000 µM range) of glycerol in various samples.
Features & Benefits	• Simple procedure; takes ~ 40 minutes • Fast and convenient • The kit provides an accurate assay for measuring free glycerol in various samples
Kit Components	• Glycerol Assay Buffer • Glycerol Probe (in DMSO, anhydrous) • Glycerol Enzyme Mix (lyophilized) • Glycerol Standard (100 mM)
Storage Conditions	-20°C

引用文獻

Iso, T. et al., (2017) Serum levels of fatty acid binding protein 4 and fat metabolic markers in relation to catecholamines following exercise, Clinical Biochemistry, Jun.2017, https://doiorg/101016/jclinbiochem201705021

Tsao et al, Interleukin-4 regulates lipid metabolism by inhibiting adipogenesis and promoting lipolysis. J. Lipid Res., Mar 2014; 55: 385 – 397.

MacDonald et al., IL-6 and epinephrine have divergent fiber type effects on intramuscular lipolysis. J Appl Physiol, Nov 2013; 115: 1457 – 1463.

Tetsuro Izumi et al., Activin Receptor-Like Kinase 7 Suppresses Lipolysis to Accumulate Fat in Obesity Through Downregulation of Peroxisome Proliferator–Activated Receptor and C/EBPα. Diabetes, Jan 2013; 62: 115 – 123.

Özlem Gögebakan et al., Glucose-Dependent Insulinotropic Polypeptide Reduces Fat-Specific Expression and Activity of 11β-Hydroxysteroid Dehydrogenase Type 1 and Inhibits Release of Free Fatty Acids, Diabetes, Feb 2012; 61: 292 – 300.

HDL高密度脂蛋白/ LDL低密度脂蛋白/非常低密度脂蛋白檢測試劑盒 | K613-100

Acetoacetate Colorimetric Assay Kit

HDL（高密度脂蛋白） – 膽固醇和LDL（低密度脂蛋白） – 膽固醇的調節在各種疾病發展中扮演核心作用。眾所周知，低水平的HDL和高水平的LDL與心血管事件風險增加有關。 BioVision的HDL和LDL / VLDL膽固醇定量試劑盒在血清樣品中將LDL和VLDL（極低密度脂蛋白）方便分離後，提供HDL和LDL / VLDL的簡單定量方法。在測定中，膽固醇氧化酶特異性識別游離膽固醇並產生與探針反應以產生顏色（570nm）和螢光（Ex / Em = 538/587nm）的產物。膽固醇酯酶將膽固醇酯水解成游離膽固醇，因此膽固醇酯和游離膽固醇可以在反應中膽固醇酯酶存在和不存在的情況下分別檢測。

Cat # +Size	K613-100
Size	100 assays
Detection Method	Fluorescence Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity	Mammalian
Applications	N/A
Features & Benefits	• Simple procedure; takes ~1 hour • Fast and convenient
Kit Components	• Cholesterol Assay Buffer • 2X LDL/VLDL Precipitation Buffer • Cholesterol Probe (in DMSO, anhydrous) • Enzyme Mix (Lyophilized) • Cholesterol Esterase (Lyophilized) • Cholesterol Standard (2 µg/µl)
Cat # +Size	K597-100
Size	100 assays
Detection Method	Absorbance (OD 450 nm).
Applications	Measurement of Succinyl-CoA Synthetase activity in various tissues/cells Analysis of cell signaling pathway
Features & Benefits	• Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.
Kit Components	• SCS Assay Buffer • SCS Substrate Mix (Lyophilized) • SCS Enzyme Mix (Lyophilized) • SCS Developer (Lyophilized) • NADH Standard (Lyophilized) • SCS Positive Control (Lyophilized)

LDL攝取分析試劑盒（細胞） | OxLDL 氧化低密度脂蛋白檢測 | HDL高密度脂蛋白/ LDL低密度脂蛋白/非常低密度脂蛋白檢測試劑盒