總膽固醇和膽固醇酯比色測定試劑盒II | 貨號K623-100

Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II

產品描述：Cholesterol膽固醇是所有動物生命中必不可少的分子。它涉及normal development正常發育和diseases疾病。Cholesterol/Cholesteryl Ester Quantitation Kit膽固醇/膽固醇酯定量試劑盒提供了一種使用比色法靈敏定量free cholestero游離膽固醇，cholesteryl esters膽固醇酯或兩者的簡單方法。血液中的大多數膽固醇是cholesteryl esters膽固醇酯的形式。這些酯可在適當條件下水解成free cholesterol游離膽固醇。在該測定中，free cholesterol游離膽固醇被cholesterol dehydrogenase膽固醇脫氫酶氧化以產生NADH，其與敏感探針反應，導致450nm處的強吸收。該測定可以檢測free cholesterol游離膽固醇或total cholesterol總膽固醇，這取決於酯酶是否用於水解存在的膽固醇酯。膽固醇酯可以通過從總膽固醇（膽固醇加膽固醇酯）中減去游離膽固醇的值來確定。該試劑盒中的探針更穩定，靈敏且特異。該測定可以顯著地耐受來自各種樣品的干擾。

Cat # +Size	K623-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Species Reactivity	Mammalian
Applications	N/A
Features & Benefits	• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains the necessary reagents for measurement of cholesterol in various biological samples
Kit Components	• Cholesterol Assay Buffer • Substrate Mix (lyophilized) • Enzyme Mix (lyophilized) • Esterase (lyophilized) • Cholesterol Standard (2 µg/µl)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack