【高遷移率族蛋白1檢測】High Mobility Group Protein 1 (HMGB1) 分析商品
High Mobility Group Protein 1 (HMGB1) 高遷移率族蛋白1
HMGB1高遷移率族蛋白1是35年前發現的存在於真核細胞核內的非組蛋白染色體結合蛋白，因其在聚丙烯酞胺凝膠電泳（PAGE）中遷移速度快而得名.HMGB1是一個含有215個氨基酸殘基 的單鏈多肽，高度保守，N端富含帶正電荷的賴氨酸，C末端富含帶負電荷的天門冬氨酸和谷氨酸，又稱酸性尾巴，分子量約為24894 Da。DNA結合蛋白與染色質結合併具有 bend DNA的能力。 優先結合單鏈DNA( single-stranded DNA)。 通過充當RAG複合物的輔因子參與V（D）J重組。 通過在保守重組信號序列（RSS）的23bp間隔區刺激切割和RAG蛋白結合作用。 肝素結合蛋白，其在發育中的細胞中延伸neurite-type cytoplasmic processes神經突類型的細胞質過程中扮演重要作用。 Uniprot：P09429 NCBI：NM_002128 NP_002119.1 Cloud clone提供一系列高遷移率族蛋白1（HMGB1）多株抗體、高遷移率族蛋白1（HMGB1）重組蛋白、高遷移率族蛋白1檢測試劑盒 , 提供HMGB1完整之分析。
Recombinant High Mobility Group Protein 1 (HMGB1) 重組高遷移率族蛋白1（HMGB1）重組蛋白 | 貨號RPA399Mu01
產品介紹: Cloud clone提供原核及真核生物表現之重組蛋白系列商品；其中Recombinant High Mobility Group Protein 1 (HMGB1) 重組蛋白。Organism Species為Mus musculus (Mouse)。來源為Prokaryotic expression; HOST來源為E.coli。預測分子量為26.2kDa; Accurate Molecular Mass 33kDa (Analysis of differences refer to the manual); Met1~Glu215 with N-terminal His Tag。內毒素<1.0EU per 1µg (LAL method偵測); 細胞表現位置Nucleus, Cytoplasm, Chromosome。Isoelectric Point5.6。純度> 90%，提供凍乾型式包裝。可應用於Positive Control; Immunogen; SDS-PAGE; WB.等不同研究。
蛋白質保存型式
蛋白質溶於PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. 為冷凍乾燥包裝。使用前Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL避免vortex. 短期保存Store at 2-8°C for one month.分裝-80°C長期12 個月。請避免反覆冷凍解凍。
產品附贈Positive control | Loading抗體 | SABC Kit (三擇一)
Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)多株抗體 | 貨號PAA399Mu01
產品介紹: 高遷移率族蛋白1（HMGB1）多株抗體，免疫原為RPA399Mu01-Recombinant High Mobility Group Protein 1 (HMGB1)可應用於Mus musculus (Mouse) 物種。此抗體經由Antigen-specific affinity chromatography純化，保存於0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. 抗體濃度為0.25mg/ml，最佳實驗濃度請參考操作手冊。抗體之保存: 避免反覆冷凍解凍；4°C保存；若需長期保存請貯存於-20°C。可應用於WB; IHC; ICC; IP.實驗。
Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
ELISA Kit for High Mobility Group Protein 1 (HMGB1)
商品: 高遷移率族蛋白1 檢測試劑盒 | 貨號SEA399Mu。應用物種Mus musculus (Mouse)；偵測範圍46.88-3,000pg/mL, 最低靈敏度18.29pg/mL. 可應用於Serum, plasma and other biological fluids生物樣品之檢測。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。
高遷移率族蛋白1檢測試劑盒 (酶聯免疫吸附試驗法)套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
相關抗體Beta-Actin單株抗體 | GAPDH多株抗體 | Lamin B1多株抗體 | Tubulin Beta單株抗體 | PCNA單株抗體 | Histone H3多株抗體
高遷移率族蛋白1多株抗體 更多單多株抗體/重組蛋白/ELISA套組
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA399Mu01 | Recombinant High Mobility Group Protein 1 (HMGB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA399Mu02 | Recombinant High Mobility Group Protein 1 (HMGB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA399Mu02 | Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB; IHC; ICC; IP. |
| PAA399Mu01 | Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB; IHC; ICC; IP. |
| LAA399Mu71 | Biotin-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB; IHC; ICC. |
| LAA399Mu81 | FITC-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB; IHC; ICC; IF. |
| SEA399Mu | ELISA Kit for High Mobility Group Protein 1 (HMGB1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Apoptosis系列抗體 (BAD)多株抗體 | (Bax)多株抗體 | (Bcl2)多株抗體 | (CASP3)多株抗體 | Angiopoietin 2 (ANGPT2)多株抗體 | (ANGPT1)多株抗體
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