Cell Surface Protein Isolation Kit 細胞表面蛋白分離試劑盒 | 貨號K295  (組織樣品來源請參考 Minute™單細胞分離試劑盒貨號SC-012 )

Simple and efficient method for isolation of cell surface proteins

產品描述：細胞表面蛋白(Cell surface proteins)在信號轉導( signal transduction)，細胞粘附(cell adhesion)，轉運(transport )中起主要作用，並作為診斷(diagnostic)和藥理學(pharmacological targets)靶點。 BioVision的細胞表面蛋白分離試劑盒提供了一種簡單有效的細胞表面蛋白分離方法。 在該方法中，首先用Sulfo-NHS-SS生物素（ Sulfo-NHS-SS-Biotin), 胺反應性 (thiol-cleavable)，硫醇可切割的生物素化試劑(thiol-cleavable）標記細胞。 隨後細胞裂解，使用鏈黴抗生物素蛋白珠(Streptavidin beads)分離標記的細胞表面蛋白質。 然後通過與DTT溶液孵育從珠粒釋放結合的蛋白質。 生物素化試劑是具有延伸間隔臂的細胞膜不可滲透的，以減少與鏈黴親和素結合相關的空間障礙。 這種方便的試劑盒提供了所有必需的組分，用於細胞表面蛋白的最佳標記和分離。

Cat # +Size	K295-10
Size	10 reactions
Detection Method	Western Blotting
Applications	Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies
Features & Benefits	• Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins
Kit Components	• Quenching Solution • Lysis Buffer • Wash Buffer • Streptavidin Beads • PBS Tablet • TBS Tablet • Sulfo-NHS-SS-Biotin • DTT

 Minute™單細胞分離試劑盒| (For fresh and fixed tissues) 貨號Cat# SC-012

產品介紹: Invent Biotechnologies Minute™單細胞分離試劑盒 ( Minute™ Single Cell Isolation Kit) ，具有優化的組織解聚緩衝液 (tissue disaggregation buffers)，和特殊設計2.0ml收集管的濾筒技術 ( filter cartridges) 組成。該試劑盒應用於高細胞活力的新鮮動物組織中 ( fresh animal tissues) 快速分離單細胞 ( single cells ) 。它也可用於從用甲醛(formaldehyde)短暫固定的染色體免疫沉澱（ChIP）中分離細胞。將組織解聚緩衝液 (tissue disaggregation buffers) 配製成輕輕地分解動物組織。緩衝液不含任何可能對細胞表面標記物 (cell surface markers) 有不良影響的蛋白酶 ( proteinases) 和EDTA。由於使用具有預定孔徑的filter cartridges濾筒技術和專門配製的緩衝系統，操作可以在不到10分鐘內，從新鮮組織獲得單細胞懸浮液。試劑盒的功效具有組織型依賴性( tissue type dependent)；該試劑盒對淋巴組織如脾臟 (spleen)、胸腺(thymus) 、和淋巴結 (lymph node) 特別有效。對於具有大量結締組織含量的新鮮組織，效果是可變的 (variable)。不適用於新鮮的組織如肌肉(muscle)，腸(intestine) 和肺 (lungs)。從冷凍/新鮮組織中獲取，請參閱貨號CS-031。

產品應用：提取的單細胞可用於染色體免疫共沉澱（ChIP）和FACS分析。單細胞懸浮液也可用於分離/純化DNA，RNA，蛋白和其他細胞組分。特點：簡單、快速、效率高，不含任何蛋白酶，可從新鮮/冷凍/甲醛固定的動物組織中分離單細胞。儲存條件：4℃

Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性；用於總蛋白提取，細胞分離和膜蛋白分離，只需簡單步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。

Single Cell # Per 50 mg fresh mouse tissue and viability

Tissues	Brain	Kidney	Liver	Lung	Spleen
Cell # in Millions	4-5	5-6	5-6	6-7	15-16
Viability (%)	40-50	50-55	70-75	70-80	90-95

Single Cell# Per 50 mg formaldehyde fixed mouse tissue

Tissues	Brain	Kidney	Liver	Lung	Spleen
Cell # in Millions	4-5	4-5	4-5	5-6	13-15

Item	Amount	Part #
Buffer A (for non-fixed tissues)	25 ml	SC-012-N
Buffer B (for fixed tissues)	25 ml	SC-012-F
Cell isolation filter cartridges	50	P-001
Collection Tubes with Cap	50	P-002
plastic rods	4	P-003

1. Kuo C-T, et al. (2016). Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots. Nature Communications. http://www.nature.com/naturecommunications.
2. Bultmann-Mellin, I., Dinger, K., Debuschewitz, C., Loewe, K. M., Melcher, Y., Plum, M. T., … & Jüngst, C. (2017). Role of LTBP-4 in alveolarization, angiogenesis and fibrosis in lungs. American Journal of Physiology-Lung Cellular and Molecular Physiology, ajplung-00031.
3. Vergara, M. N., Flores-Bellver, M., Aparicio-Domingo, S., McNally, M., Wahlin, K. J., Saxena, M. T., … & Canto-Soler, M. V. (2017). Enabling quantitative screening in retinal organoids: 3D automated reporter quantification technology (3D-ARQ). Development, dev-146290.
4. Janicova, A., Becker, N., Xu, B., Wutzler, S., Vollrath, T., Hildebrand, F., … & Relja, B. (2019). Endogenous uteroglobin as intrinsic anti-inflammatory signal modulates monocyte and macrophage subsets distribution upon sepsis induced lung injury. Frontiers in Immunology, 10, 2276.
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Cat. No	Starting Materials	Status of Extracted Proteins	Yield (mg/ml)	Protocol Time (min)
SD-001/ SN-002	Cultured Animal Cells and Tissues	Denatured/Native	2-8	1-8
SC-003	Mammalian Cultured Cells/Tissues	Native	1-3	6-8
SN-006	Cultured Animal Cells and Tissues	Denatured/Native	1-4	5-8
SM-005	Mammalian Cultured Cells/Tissues	Native Detergent/ EDTA -Free	1-3	20-45
MP-007	Mammalian Cultured Cells/Tissues	Native Detergent/ EDTA-Free	1-3	25-30
SD-008/ SN-009	Fresh Plant Tissues	Denatured/Native	2-8	5-8
SN-010	Fresh Plant Tissues	Native	1-5	6-8
CP-011	Fresh Plant Tissues (5-200 mg/sample)	Chloroplast (>90%) Intact	1-4	5
SB-004	Cultured Bacterial Cells	Denatured	2-3	2-3
NE-013	Cultured cells or Cells from Tissues	Native	1-3	45
HP-014	Cultured cells or Cells from Tissues	Denatured	1-2.5	<10
YT-015	Cultured Microbes	Denatured/Native	2-5	<10 min
YD-016	Cultured Microbes	Denatured/Native	2-4	<10 min
YM-017	Cultured Yeast	Native	2-3	1h
MM-018	Plant tissues	Native	2-3	1h
PN-019	PAGE/Agarose gels	Denatured/Native	NA	10-20 min
HD-021	Keratinized tissues	Denatured	1-2	5 min hand on
AT-022	Adipose Tissues/Adipocytes	Native/Denatured	2-3	20 min
AF-023	Adipose Tissues/Adipocytes	Native	0.5-1	20 min
FE-025	FFPE	Denatured	2-3	1h

Sub-Cellular Structure Fractionation/Isolation
Cat #	Description	Size
SC-003	Cytoplasmic & Nuclear Extraction Kits	50 Preps
SM-005	Plasma Membrane Protein Isolation Kit	50 preps
NE-013	Nuclear Envelope Protein Extraction Kit	50 preps
MP-007	Mitochondria Isolation Kit For Mammalian Cells And Tissues	50 preps
AF-023	Adipose Tissue Fractionation Kit	20 preps
NI-024	Detergent-free nuclei isolation kit	20 preps
YM-017	Yeast Mitochondria Enrichment Kit	50 preps
MM-018	Plant Microsomal Membrane Extraction Kit	50 preps
CP-011	Chloroplast Isolation Kit	50 preps