Minute™單細胞分離試劑盒|Single Cell Isolation Kit貨號Cat# SC-012
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Minute™單細胞分離試劑盒| (For fresh and fixed tissues) 貨號Cat# SC-012
產品介紹: Invent Biotechnologies Minute™單細胞分離試劑盒 ( Minute™ Single Cell Isolation Kit) ，具有優化的組織解聚緩衝液 (tissue disaggregation buffers)，和特殊設計2.0ml收集管的濾筒技術 ( filter cartridges) 組成。該試劑盒應用於高細胞活力的新鮮動物組織中 ( fresh animal tissues) 快速分離單細胞 ( single cells ) 。它也可用於從用甲醛(formaldehyde)短暫固定的染色體免疫沉澱（ChIP）中分離細胞。將組織解聚緩衝液 (tissue disaggregation buffers) 配製成輕輕地分解動物組織。緩衝液不含任何可能對細胞表面標記物 (cell surface markers) 有不良影響的蛋白酶 ( proteinases) 和EDTA。由於使用具有預定孔徑的filter cartridges濾筒技術和專門配製的緩衝系統，操作可以在不到10分鐘內，從新鮮組織獲得單細胞懸浮液。試劑盒的功效具有組織型依賴性( tissue type dependent)；該試劑盒對淋巴組織如脾臟 (spleen)、胸腺(thymus) 、和淋巴結 (lymph node) 特別有效。對於具有大量結締組織含量的新鮮組織，效果是可變的 (variable)。不適用於新鮮的組織如肌肉(muscle)，腸(intestine) 和肺 (lungs)。從冷凍/新鮮組織中獲取，請參閱貨號CS-031。
產品應用：提取的單細胞可用於染色體免疫共沉澱（ChIP）和FACS分析。單細胞懸浮液也可用於分離/純化DNA，RNA，蛋白和其他細胞組分。特點：簡單、快速、效率高，不含任何蛋白酶，可從新鮮/冷凍/甲醛固定的動物組織中分離單細胞。儲存條件：4℃
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性；用於總蛋白提取，細胞分離和膜蛋白分離，只需簡單步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。
Single Cell # Per 50 mg fresh mouse tissue and viability
| Tissues | Brain | Kidney | Liver | Lung | Spleen |
| Cell # in Millions | 4-5 | 5-6 | 5-6 | 6-7 | 15-16 |
| Viability (%) | 40-50 | 50-55 | 70-75 | 70-80 | 90-95 |
Single Cell# Per 50 mg formaldehyde fixed mouse tissue
| Tissues | Brain | Kidney | Liver | Lung | Spleen |
| Cell # in Millions | 4-5 | 4-5 | 4-5 | 5-6 | 13-15 |
| Item | Amount | Part # |
| Buffer A (for non-fixed tissues) | 25 ml | SC-012-N |
| Buffer B (for fixed tissues) | 25 ml | SC-012-F |
| Cell isolation filter cartridges | 50 | P-001 |
| Collection Tubes with Cap | 50 | P-002 |
| plastic rods | 4 | P-003 |
- Kuo C-T, et al. (2016). Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots. Nature Communications. http://www.nature.com/naturecommunications.
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| Cat. No | Starting Materials | Status of Extracted Proteins | Yield (mg/ml) |
Protocol Time (min) |
| SD-001/ SN-002 | Cultured Animal Cells and Tissues | Denatured/Native | 2-8 | 1-8 |
| SC-003 | Mammalian Cultured Cells/Tissues | Native | 1-3 | 6-8 |
| SN-006 | Cultured Animal Cells and Tissues | Denatured/Native | 1-4 | 5-8 |
| SM-005 | Mammalian Cultured Cells/Tissues | Native Detergent/ EDTA -Free | 1-3 | 20-45 |
| MP-007 | Mammalian Cultured Cells/Tissues | Native Detergent/ EDTA-Free | 1-3 | 25-30 |
| SD-008/ SN-009 | Fresh Plant Tissues | Denatured/Native | 2-8 | 5-8 |
| SN-010 | Fresh Plant Tissues | Native | 1-5 | 6-8 |
| CP-011 | Fresh Plant Tissues (5-200 mg/sample) | Chloroplast (>90%) Intact | 1-4 | 5 |
| SB-004 | Cultured Bacterial Cells | Denatured | 2-3 | 2-3 |
| NE-013 | Cultured cells or Cells from Tissues | Native | 1-3 | 45 |
| HP-014 | Cultured cells or Cells from Tissues | Denatured | 1-2.5 | <10 |
| YT-015 | Cultured Microbes | Denatured/Native | 2-5 | <10 min |
| YD-016 | Cultured Microbes | Denatured/Native | 2-4 | <10 min |
| YM-017 | Cultured Yeast | Native | 2-3 | 1h |
| MM-018 | Plant tissues | Native | 2-3 | 1h |
| PN-019 | PAGE/Agarose gels | Denatured/Native | NA | 10-20 min |
| HD-021 | Keratinized tissues | Denatured | 1-2 | 5 min hand on |
| AT-022 | Adipose Tissues/Adipocytes | Native/Denatured | 2-3 | 20 min |
| AF-023 | Adipose Tissues/Adipocytes | Native | 0.5-1 | 20 min |
| FE-025 | FFPE | Denatured | 2-3 | 1h |
| Sub-Cellular Structure Fractionation/Isolation | |||
| Cat # | Description | Size | |
| SC-003 | Cytoplasmic & Nuclear Extraction Kits | 50 Preps | |
| SM-005 | Plasma Membrane Protein Isolation Kit | 50 preps | |
| NE-013 | Nuclear Envelope Protein Extraction Kit | 50 preps | |
| MP-007 | Mitochondria Isolation Kit For Mammalian Cells And Tissues | 50 preps | |
| AF-023 | Adipose Tissue Fractionation Kit | 20 preps | |
| NI-024 | Detergent-free nuclei isolation kit | 20 preps | |
| YM-017 | Yeast Mitochondria Enrichment Kit | 50 preps | |
| MM-018 | Plant Microsomal Membrane Extraction Kit | 50 preps | |
| CP-011 | Chloroplast Isolation Kit | 50 preps | |