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PurKine™ 抗體純化試劑盒（蛋白 A/G）PurKine™ Antibody Purification Kit (Protein A/G) | 貨號KTP2070

PurKine™ Antibody Purification Kit (Protein A/G)

PurKine™ Protein A/G Resin 4FF 已被固定用於各種規模的重力柱程序中的 IgG 純化應用。該樹脂由 90μm 的交聯 4% 瓊脂糖珠組成，並與重組蛋白 A/G 偶聯。測試證實其性能等於或超過其他供應商的流行重組蛋白 A/G 樹脂，並且在使用後性能沒有下降至少五次重複使用。此外，它的高流動性使其非常適合按比例放大。

PurKine™ 蛋白 A/G 純化系統(PurKine™ Protein A/G Purification system)可有效地從血清、腹水、細胞培養上清液和其他抗體樣品中親和純化 IgG。重組蛋白 A/G (Recombinant Protein A/G)採用專有方法進行化學修飾，以最大限度地減少蛋白質的非特異性結合。此外，Protein A/G 與免疫球蛋白的結合不像 Protein A 那樣依賴於 pH。Protein A/G 與所有人類 IgG 亞類結合併與所有小鼠 IgG 亞類很好地結合，但不與小鼠 IgA、IgM 或血清白蛋白結合。該產品組合允許優化工藝以獲得最大的蛋白質產量、穩定性和溶解度。測試還證實，在至少五次重複使用後性能沒有下降。 PurKine™ 蛋白 A/G 純化樹脂也可作為預裝離心柱(prepacked spin column)和試劑盒形式(kit formats)提供。

Product name	PurKine™ Antibody Purification Kit (Protein A/G)
Applications notes	PurKine™ Protein A/G Resin 4FF has been immobilized for use in IgG purification applications in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein A/G has been coupled.Tests confirm that performance equals or exceeds popular Recombinant protein A/G resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.In addition, its high flow properties make it excellent for scaling-up.
Alternative	Protein A/G; Protein A/G resin
Kit components	• PurKine™ Protein A/G Packed Column • Binding/Wash buffer(10X) • Elution buffer(10x)
Features & Benefits	• High capacity—More than 7mg human lgG per mL of resin. • High performance—Recombinant Protein A/G Beads combines the IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more general and convenient tool for investigating and purifying immunoglobulins. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. • Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour. • Easy to use— Pre-formulated buffers available for kit formats.
Storage instructions	Stable for one year at 2-8°C from date of shipment. Do not freeze.
Background	PurKine™ Protein A/G Purification system is effective for affinity purification of IgG from serum, ascites fluid, cell culture supernantant and other antibody samples. Recombinant Protein A/G is chemically modified with a proprietary method to minimize nonspecific binding of proteins. Also, Protein A/G binding to immunoglobulins is not as pH-dependant as Protein A. Protein A/G binds to all human IgG subclasses and binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein A/G Purification Resin is also available as prepacked spin column and kit formats.
Alternative	Protein A/G; Protein A/G resin

 

PurKine™ His-Tag Ni-NTA Resin樹脂 | 貨號BMR2001

PurKine™ His-Tag Ni-NTA Resin

PurKine™ His-Tag 純化系統基於創新的高容量 IMAC 基質(IMAC matrix)，可方便地從總裂解物(total lysates)中一步純化 His-tag 蛋白(His-tag proteins)。我們專有的離子螯合化學(ion-chelate chemistry)確保與常用的還原劑（如 DTT）、螯合金屬蛋白酶抑製劑（chelating metalloprotease inhibitors, 如 EDTA）以及各種緩衝物(buffer substances)質和鹽條件具有非凡的相容性。該產品組合提供多種螯合基團（chelating group , IDA、NTA 或 Super NTA）、交聯瓊脂糖（交聯 4% 或 6% 瓊脂糖）、離子（Nick、Colbat、銅或非帶電離子）和兼容成分，以實現優化最大蛋白質產量、穩定性和溶解度的過程。測試還證實，在至少五次重複使用後性能沒有下降。 PurKine™ His-Tag 純化樹脂也可作為預裝離心柱(prepacked spin column)和試劑盒(kit)形式提供。PurKine™ His-Tag Ni-NTA Resin 在各種規模的重力柱(gravity column)程序中有效純化高水平過表達的 His-tagged 融合蛋白。該樹脂(Resin)由 90μm 高度交聯的 4% 瓊脂糖珠(cross-linked 4% agarose)組成，與硝酸三乙酸 (Nitilotriacetic acid , NTA) 偶聯。然後螯合基團帶有鎳離子 (nickel ions , Ni2+)。測試證實其性能等於或超過其他供應商的流行 Ni-NTA 樹脂，並且在至少五次重複使用後性能沒有下降。

Product name	PurKine™ His-Tag Ni-NTA Resin
Applications notes	PurKine™ His-Tag Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
Alternative	Ni-NTA; His resin; Ni-NTA resin; His tag resin
Formulation	Liquid solution with 50% slurry in 20% ethanol.
Features & Benefits	• High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin. • Versatile—For purification under native or denaturing conditions. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
Storage buffer	Liquid in PBS buffer containing 20% ethanol.
Storage instructions	Stable for one year at 2-8°C from date of shipment. Do not freeze.
Shipping	Blue ice
Background	PurKine™ His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ His-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative	Ni-NTA; His resin; Ni-NTA resin; His tag resin

免疫沉澱套組 | Universal IP/Co-IP Toolkit (Agarose) 貨號 KTD105-EN

Universal IP/Co-IP Toolkit (Agarose)

免疫沉澱（Immunoprecipitation，IP）是一種小型親和純化方法(affinity purification method)，使用固定在磁珠(magnetic beads)或瓊脂糖(agarose)等固相支持物(solid-phase supports)上的特異性抗體。免疫沉澱 (Immunoprecipitation, IP)作為許多蛋白質組學相關研究的重要組成部分，可用於檢測蛋白質(proteins)的存在、相對豐度(abundance)、蛋白質的上下表達、蛋白質的穩定性和相互作用等。Abbkine開發了Universal IP/Co-IP Toolkit（Agarose）；該工具包經過設計和研究，包含：優化的天然和變性裂解物(optimized natural and denatured lysate)、蛋白A/G瓊脂糖(protein A/G Agarose)、IP陰性對照(IP negative control)、IPkine^TM二抗(second antibody)，可滿足大多數用戶的IP/Co-IP需求。

高效(Efficient)：高效的抗體結合能力和低蛋白非特異性吸附率，節省抗體消耗。

1. 方便通用(Convenient and universal)：包含IP/Co-IP 和WB 實驗所需的所有緩衝液，可同時滿足樣品IP/Co-IP 或WB 的要求。
2. 可靠穩定(Reliable and stable)：含有即用型IP陰性對照(IP negative control)，可消除IgG自身與靶蛋白或其他特異性生物分子的非特異性結合，保證IP抗體的特異性，並含有獨特的IPkine™二抗完美消除重鏈(heavy chain interference)干擾。

Abbkine BCA蛋白定量試劑盒 | 貨號KTD3001 50-1000 µg/ml

Abbkine 蛋白定量試劑盒 (BCA 分析) 為總蛋白質的色度檢測和定量提供了一個簡單、快速、與洗滌劑相容的程式。該方法利用鹼性介質中蛋白質減少銅陽離子(Cu2+)到銅陽離子(Cu+)。生成的 Cu+ 與二面體酸 (BSA) 形成強烈的紫色複合物,在 562nm 時具有極強的吸收力。水溶性複合物的強度與樣品中的蛋白質量成正比。BCA蛋白測定適用於50-1000μg/ml範圍內的蛋白質濃度測量。

BCA (Bicinchoninic Acid , BCA) 在鹼性環境Cu+ 由鹼性Cu2+蛋白質的反應產生的。由此產生的反應和顏色是一種普通蛋白質定量方法的基礎,該方法能夠測量各種蛋白質濃度。增加蛋白質濃度會產生比例更深的顏色。BCA蛋白測定與勞里技術相比,BCA蛋白測定對普通干擾物質(如非離子洗滌劑和緩衝鹽)的耐受性更高。

Abbkine 蛋白定量試劑盒產品特點:

1. 節省時間: 比傳統的Lowry method方法更容易,快四倍。
2. 靈敏度: 檢測至 25 μg/ml,檢測蛋白的最小量達到5μg。
3. 相容性: 不受大多數離子和非離子洗滌劑的影響。可與高達 5 % SDS、 5 % Triton X-100、 5 % Tween-20、 60、 80 在樣品中相容。
4. 良好的線性度: 線性工作範圍從 50 到 1000 μg/ml.
5. 與染料結合方法相比,蛋白質到蛋白質的變異性較少。

Fig. Abbkine Protein Quantification Kit (BCA Assay) standard curve

• Kit components
• BCA Reagent A (2x) • BCA Reagent B • BSA Standard
• Features & Benefits
• Time saving—much easier and four times faster than the classical Lowry method. • Sensitive—detect down to 25 µg/ml and the minimum amount of detection protein reaches 0.5μg. • Compatible—unaffected by most ionic and nonionic detergents. Can be compatible with up to 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 in the sample. • Good linearity—linear working range from 50 to 1000 µg/ml. • Excellent uniformity— exhibits less protein-to-protein variation than dye-binding methods.
• Calibration range

50-1000 µg/ml

• Limit of detection

25 µg/ml

• Usage notes
• The color of the reaction is not only related to the protein concentration of the sample, but also to the temperature of the reaction. If the concentration of protein in the sample is high (＞50μg/ml), the reaction temperature is usually 37℃. If the concentration of protein in the sample is low (< 50μg/ml), the reaction temperature is 60℃. At this temperature, tryptophan, tyrosine and peptide bonds are fully oxidized, greatly improving the detection sensitivity.
  • Commonly used concentrations of detergent SDS, Triton X-100, Tween do not affect the detection results, but results are affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. If such substance exists in the sample, try the Bradford method.

注意事項:

1. 反應的顏色不僅與樣品的蛋白質濃度有關,而且與反應的溫度有關。如果樣品中的蛋白質濃度高(±50μg/ml),反應溫度通常為37°C。如果樣品中的蛋白質濃度低(=50μg/ml),反應溫度為60°C。在此溫度下,色氨酸、酪氨酸和肽鍵完全氧化,大大提高了檢測靈敏度。
2. 常用的洗滌劑 SDS、Triton X-100、Tween 濃度不影響檢測結果,但結果受包紮劑(EDTA、EGTA)、還原劑(DTT、甲醇)和脂質影響。如果樣品中存在此類物質,請嘗試布拉德福德方法。

試劑4°C至少穩定12個月。 BCA試劑A和BCA 試劑B在4°C下穩定。BSA標準品自第一次融化後，應將BSA標準等分。 儲存在-20°C。

引用文獻

Chronic inhibition of chemokine receptor CXCR2 attenuates cardiac remodeling and dysfunction in spontaneously hypertensive rats

YL Zhang, C Geng, J Yang, J Fang, X Yan, PB Li. Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease, 2019.

技術提示

為避免交叉污染，添加試劑之間，添加樣品之間都需更換Tip。另外，每種試劑都應使用單獨的容器。BCA試劑B與BCA試劑A稀釋液混合後，觀察到渾濁混合後迅速消失，得到透明的綠色BCA工作溶液。BCA孵育時間和溫度都可以在相當廣泛的範圍內變化。使用更高的溫度和更長的孵育時間，蛋白質含量較低的樣品更容易定量。已知某些物質會干擾BCA分析，包括那些降低電位，螯合劑和強酸或強鹼。因為他們是已知即使在極低的濃度下也會干擾蛋白質估計，避免這些物質作為樣品緩衝液的成分。某些物質在較小程度上干擾了使用BCA進行蛋白質估算分析，並且在低於特定濃度的情況下，這些僅具有較小的（可忍受的）影響，如NP-40，SDS，Triton X-100，Tween-20等。在含有去污劑的溶液中測定蛋白質時，可通過以下方法獲得最佳結果向含有蛋白質標準品的孔中添加相同量的去污劑。

PurKine™內毒素去除樹脂(套組)  | PurKine™ Endotoxin Removal Kit (polymyxin B)貨號KTP2140

PurKine™ Endotoxin Removal Kit (polymyxin B)套組包括預裝離心Column、buffer

• PurKine™ Endotoxin Removal polymyxin B Packed Column
• Regeneration buffer(10X)
• Equilibration buffer(10x)

PurKine™內毒素去除樹脂(PurKine™ Endotoxin Removal Resin) 可有效降低蛋白質樣品中的內毒素(Endotoxins)水平。內毒素(Endotoxins)，也稱為脂多醣(lipopolysaccharides)或LPS，是革蘭氏陰性細菌（Gram-negative bacteria）, 例如大腸桿菌(E. coli）的細胞膜成分(cell membrane components)。內毒素(Endotoxins)強烈影響DNA轉染至原代細胞(primary cells)和敏感培養細胞中，內毒素(Endotoxins)水平升高導致轉染效率(transfection efficiencies)急劇降低。樹脂(Resin)由90μm交聯的4％瓊脂糖珠(cross-linked 4% agarose)組成，且由修飾的多粘菌素B(modified polymyxin B)與之偶聯。該產品組合具優化(optimization)，以實現最大的蛋白質產量(protein yield)，穩定性(stability)和溶解性(solubility)。測試確認，至少重複使用五次後。性能不會降低。PurKine™內毒素去除系列產品，有提供樹脂(PurKine™內毒素去除樹脂, 貨號BMR2140)、預裝離心柱(PurKine™內毒素去除預裝離心柱, BMC2140) 和試劑盒(PurKine™內毒素去除樹脂(套組)  (polymyxin B), KTP2140) 三種商品形式提供。

產品描述： PurKine™內毒素去除試劑盒(PurKine™ Endotoxin Removal Kit (polymyxin B), KTP21400) 旨在將蛋白質樣品中的內毒素(Endotoxins)水平降低≥99％，同時最大限度地提高蛋白質回收率。樹脂(Resin)由90μm交聯的4％瓊脂糖珠組成，修飾的多粘菌素B（PMB）已與之偶聯。測試證實其性能等於或超過其他供應商的流行多粘菌素B（PMB）樹脂，並且至少重複使用五次後，性能不會降低。操作手冊PurKine™ Endotoxin Removal Kit (polymyxin B)

PurKine™ Endotoxin Removal Resin has been designed to reduce endotoxin levels in protein samples by ≥99% while maximizing protein recovery. The Resin consists of 90μm beads of cross-linked 4% agarose, to which modified polymyxin B (PMB) has been coupled. Tests confirm that performance equals or exceeds popular polymyxin B (PMB) resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

• FormulationLiquid solution with 50% slurry in 20% ethanol.
• Kit components• PurKine™ Endotoxin Removal polymyxin B Packed Column
  • Regeneration buffer(10X)
  • Equilibration buffer(10x)
• Features & Benefits• High capacity—More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
  • High performance—Protein recovery is more than 85%.Reducing final EU concentration to 0.1 EU/mL
  • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
  • Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Storage instructionsStable for one year at 2-8°C from date of shipment. Do not freeze.

正常山羊血清(Normal Goat Serum) | 貨號BMS0050

Normal Goat Serum

產品描述：

正常山羊血清(Normal Goat Serum)，來自非免疫山羊宿主的正常山羊血清。正常血清可應用於免疫反應中之blocking，建議PBS稀釋至5％-20％（v / v）正常血清作為封閉劑(blocking reagent)，以減少非特異性結合(non-specific binding)的背景或用於大多數immunoassay applications的對照。亦可在加入一級抗體之前將Blocking reagent先進行反應。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：IHC-p（1：10-20），IF / ICC（1：10-20）和ELISA（1：100）。

• Product name

Normal Goat Serum

• Host

Goat

• Applications

BL, ELISA, ICC, IF, IHC

• Application notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).

• Preparation method

Normal goat serum is processed from blood collected from non-immunized normal adult goats. Hemolysis is minimized by employing proprietary techniques during the blood collection and serum extraction processes.

• Purification

Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2

• Purity

Whole serum

• Formulation

Liquid solution

• Storage buffer

10 mM sodium phosphate, 0.15 M sodium chloride with 0.05% sodium azide as preservative, pH 7.2

• Storage instructions

Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.

• Shipping

Gel pack with blue ice.

產品貨號	產品品項	反應	測量儀器
貨號KTP2070	抗體純化試劑盒（蛋白 A/G）	1ml
貨號BMR2001	His-Tag Ni-NTA Resin樹脂	5ml
貨號 KTD105-EN	IP/Co-IP Toolkit (Agarose)	20個反應
貨號KTD3001	BCA蛋白定量試劑盒	500個反應	OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml.
貨號KTP2140	PurKine™內毒素去除樹脂(套組)	1ml×5	More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
BMD0062	Hoechst 33342	10MG
BMU104-EN	增強型Antifade封片劑	10ml
貨號BMS0050	正常山羊血清	10ml