Cytochrome c 釋放細胞凋亡分析套組 | 貨號K257-100

| 代理廠牌: | ![]() |
| 原廠連結: | |
| 相關下載: |
特點
Cytochrome c Releasing細胞凋亡分析套組 | 貨號K257-100
Cytochrome c Releasing Apoptosis Assay Kit
產品介紹: 細胞色素c在細胞凋亡中起重要作用。 蛋白質位於內外線粒體膜之間的空間。 凋亡刺激引發細胞色素c從線粒體釋放到細胞質中,其結合Apaf-1。 細胞色素c / Apaf-1複合物激活caspase-9,然後激活caspase-3和其他下游半胱天冬酶。 BioVision的細胞色素c釋放細胞凋亡測定試劑盒,用於在細胞凋亡過程中檢測細胞色素c從線粒體轉運到細胞質中。 該試劑盒提供獨特的試劑配方,以從細胞溶質中分離高度富集的線粒體。 該方法簡單易行,無需超速離心,無毒化學物質。 然後使用試劑盒中提供的細胞色素c抗體通過Western印跡測定從線粒體釋放到細胞質中的細胞色素c。
| Size | 100 assays |
| SKU+Size | K257-100 |
| Detection Method | Western blotting |
| Species Reactivity | Mammalian |
| Applications | Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol. |
| Features & Benefits | • Simple procedure; takes ~3 hours • Fast and convenient • Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required. |
