微量型細胞凋亡DNA片段化| Apoptotic DNA Fragmentation貨號K170-50(ab65627)

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原廠連結：	http://www.biovision.com
相關下載：	Apoptotic DNA Ladder Detection Kit操作手冊

特點

Apoptotic DNA Fragmentation 細胞凋亡DNA片段化| 貨號K170-50(ab65627)

Apoptotic DNA Ladder Isolation Kit

DNA片段化為細胞凋亡的其中一項特徵。細胞凋亡活化內生性的內切酶，接著在核內將染色質DNA切斷成以180bp為倍數的小片段（360、540 etc.）。這個現象可以被例如DNA laddering assay、TUNEL assay、或Nicoletti assay用來偵測細胞凋亡。Biovision提供一系列Apoptotc DNA Fragment針對DNA ladder進行DNA萃取。貨號K170-50(ab65627)針對DNA ladder進行DNA萃取。可偵測5%以下(微量型) 凋亡的細胞或組織DNA片段。

Functional Studies – Apoptotic DNA Ladder Isolation Kit (ab65627)

DNA ladders were extracted from 2 x 10⁶ Jurkat cells were treated with 2 μM Camptothecin. The kit extracts laddered DNA only but not intact genomic DNA; therefore, more cells can be extracted and load on the agarose gel. Lane 1: 1 kb DNA ladder; Lane 2: 6 hour induction; Lane 3: 12 hour induction.

Size	50 assays
SKU+Size	K170-50 (ab65627)
Detection Method	Agarose gel electrophoresis (with Ethidium Bromide)
Species Reactivity	Mammalian
Applications	Extraction of apoptotic DNA ladders without interference of intact genomic DNA
Features & Benefits	• Simple one-step procedure; takes only 2-4 hours • Fast and convenient • The kit can extract apoptotic DNA ladder from both tissues and cells with as little as 5% or less apoptotic cell population.
Kit Components	• DNA Ladder Extraction Buffer • Enzyme A Solution • Enzyme B (Lyophilized) • Ammonium Acetate Solution • DNA Suspension Buffer

Functional Assay: ab65627 Apoptotic DNA Ladder Isolation Kit
Apoptosis induced in Jurkat cells (1e6 cell x mL-1) by incubation for 24 hours with 1) Control medium; 2) 2 uM Camptothecin (ab120115); 3) 10 uM Camptothecin; 4) 10 uM Camptothecin + 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; ab120487); 5) 50 ng x mL-1 anti-Fas Ab (MBL), 6) 0.05 mM H2O2 (Sigma); 7) 1 mM H2O2. 5 uL of DNA preparation loaded into each well.

Biovision Apoptotic DNA Ladder Detection Kits

　　Biovision生產的細胞凋亡DNA ladder分析套組，使用電泳系統進行分析。共分為三種：

- K120-50 (ab66090) Quick Apoptotic DNA Ladder Detection Kit
  - 快速偵測細胞凋亡產生的DNA片段。
  - 與市面上動輒須要1－2天操作的套組相比。只需90分鐘以內的時間即可將染色體DNA製備完成。
  - 使用單管操作，不須純化或管柱操作。
  - 增加小片段DNA的回收，並增加靈敏度。
- K130-50 (ab66093) Enhanced Apoptotic DNA Ladder Detection Kit
  - 90分鐘內快速操作。
  - 結合最佳化的buffer system及染劑，可大幅提升偵測靈敏度。
- K170-50 (ab65627) Apoptotic DNA Ladder Isolation Kit
  - 針對DNA ladder進行DNA萃取。
  - 可偵測5%以下凋亡的細胞或組織DNA片段。
  - 2-4 hours

產品編號	產品名稱	包裝
K120-50 (ab66090)	Quick Apoptotic DNA Ladder Detection Kit 1 hr 30 min Adherent cells, Suspension cells	50 assays
K130-50 (ab66093)	Enhanced Apoptotic DNA Ladder Detection Kit 1 hr 30 min Tissue, Adherent cells, Suspension cells	50 assays
K170-50 (ab65627)	Apoptotic DNA Ladder Isolation Kit 2-4 hours (Sample type: Adherent cells, Suspension cells, Tissue)	50 assays

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