HOT!! CRISPR 單株clone細胞 | 最簡單gDNA萃取方法  DirectPCR Lysis Reagent (Cell )  貨號# 302-c 100m l包裝

Direct PCR Lysis reagent 只要一個加熱步驟反應，即可將DNA由細胞中釋放。此產品具有抑制PCR抑制物的反應，因此不須考量PCR抑制問題，細胞加入reagent反應後，即可進行PCR反應。不須經由任何純化的步驟；細胞加熱完後的溶液可以直接PCR，讓genotyping分析更容易。可快速應用於CRISPR  KI 細胞之單株clone gDNA製備。

DirectPCR® system offers advantages over conventional protocols that include:

· Time saving: Less hands-on time. Crude tail lysates for PCR.
· Money saving: Cost-effective reactions.
· Safe: No organic reagents.
· Environmental: Less waste (organic reagents, tubes, tips, etc…)
· Reliable and efficient: Virtually 100% success rate with high yields.

產品介紹: VIAGEN BIOTECH提供一系列單管gDNA萃取套組，一管下完成反應；產品分為尾巴genomic DNA萃取、細胞genomic DNA萃取、耳朵genomic DNA萃取、卵黃囊genomic DNA萃取。傳統genomic DNA萃取，都須經由Phenol/Chloroform萃取步驟，或者市售genomic DNA Purification套組(通過column等繁瑣步驟)來純化genomic DNA。傳統萃取genomic DNA，無法直接PCR；因組織內含有PCR反應抑制因子，抑制PCR反應。須經由有機溶劑或kit萃取gDNA。DirectPCR Lysis Reagents具有抑制PCR抑制物的反應，不須考量PCR抑制問題。VIAGEN BIOTECH公司專門以DirectPCR Lysis Reagent銷售為事業， 其公司研發、生產、銷售。genomic DNA，PCR反應產物，可以大於15Kb，其在4度C，可以維持不變的效能。在-20度C 可以維持1年品質都不會改變。DirectPCR Lysis Reagent以粗萃取物反應為目的；若要純化Crude lysates，其方法比同一般DNA純化-DNA in crude lysates can be rescued for further analysis. Add NaCl to a final concentration of 250mM , and then add 0.7 volume of isopropanol. DNA will form precipitates. Centrifuge at 4°C for 2 min, discard supernatant, wash DNA with 1 ml 70% EtOH, and dissolve DNA in 50 μl 10 mM Tris-HCl (8.0). Use 1 μl for PCR.

Brief procedure:
1.  Lyse tails in DirectPCR® Lysis Reagent.
2.  Incubate for 45 min at 85°C.
3.  PCR genotyping with 1 µl lysates.

DirectPCR DNA Extraction Reagents	Cat#	Comments
DirectPCR Lysis Reagent (mouse tail)	[101-T]	250 mouse tails (50 ml)
DirectPCR Lysis Reagent (mouse tail)	[102-T]	500 mouse tails (100 ml)
DirectPCR Lysis Reagent (yolk sac)	[201-Y]	yolk sacs (50 ml)
DirectPCR Lysis Reagent (yolk sac)	[202-Y]	Yolk sacs (100 ml)
DirectPCR Lysis Reagent (cell	[301-C]	cultured cells (50 ml)
DirectPCR Lysis Reagent (cell)	[302-C]	cultured cells (100 ml)
DirectPCR Lysis Reagent (mouse ear)	[401-E]	mouse ear (25 ml)
DirectPCR Lysis Reagent (mouse ear)	[402-E]	mouse ear (50 ml)