商品: 生物素定量呈色分析套組Biotin Quantitation Kit (Colorimetric)  貨號K811-100  (吸收光500nm)

產品描述: 生物素（維生素H）是一種重要的生物分子，已被廣泛用於生物素化反應，標記於抗體或其他目標蛋白質。由於其低分子量，生物素通常不引起protein conformation和生物活性的任何顯著變化。生物素化的蛋白質/抗體被廣泛應用於streptavidin pull-down assay分離與分析、親和層析、ELISA、Western blot。Biovision提供多種即用的生物素化試劑（Cat。＃2346,2345,2347,2322,2326 ，2323）。通常期望確定這種生物素化的生物分子的取代程度。 Biovision的生物素測定試劑盒基於鏈黴抗生物素與生物素和染料2-（4-羥基偶氮苯）苯甲酸（HABA）的差別結合。當HABA與鏈黴親和素結合時，加入游離的生物素或生物素化的生物分子導致其在反應中的置換。這些變化表現為總吸收（OD 500nm）的降低，並且可以與溶液中存在的生物素的量定量相關。試劑盒還提供作為標準品的生物素和作為陽性對照的生物素化BSA。該試劑盒可用於估計溶液中低至60pmol或15ng的生物素。

套件組件：

• Biotin Assay Buffer
• Biotin Assay Reagent A
• Biotin Assay Reagent B
• Biotin Standard Solution (10 mM in DMSO)
• Biotinylated BSA (2 mg/ml)

K811-100	Biotin Quantitation Kit (Colorimetric)	100 assays
1124-20C	Biotin-DEVD-FMK	20 µl (10 mM)
2329-50	Biotin-HPDP	50 mg
2329-250	Biotin-HPDP	250 mg
1121-20C	Biotin-IETD-FMK	20 µl (10 mM)
2346-50	Biotin-LC-LC-NHS	50 mg
2346-250	Biotin-LC-LC-NHS	250 mg
2346-1000	Biotin-LC-LC-NHS	1 g
2345-50	Biotin-LC-NHS	50 mg
2345-250	Biotin-LC-NHS	250 mg
2345-1000	Biotin-LC-NHS	1 g
2347-50	Biotin-NHS	50 mg
2347-250	Biotin-NHS	250 mg
2347-1000	Biotin-NHS	1 g
2791-50	Biotin-PEG₄-amine	50 mg
2791-250	Biotin-PEG₄-amine	250 mg
1123-20C	Biotin-VAD-FMK	20 µl (10 mM)
1032-20C	Biotin-VDVAD-FMK	20 µl (10 mM)
1985-500	Biotinyl-ASTD-FMK	500 µg
1985-1000	Biotinyl-ASTD-FMK	1 mg
7929-250	Biotinylated AGE-BSA	250 µg
7929-1000	Biotinylated AGE-BSA	1 mg
7099-5	Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)	5 mg
7099-25	Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)	25 mg
7097-5	Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)	5 mg
7097-25	Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)	25 mg
7098-5	Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)	5 mg
7098-25	Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)	25 mg
7930-250	Biotinylated Glucose AGE-BSA-II	250 µg
7930-1000	Biotinylated Glucose AGE-BSA-II	1 mg
P1038-10	Biotinylated PD-1/PDCD1, Human Recombinant	10 μg
P1038-50	Biotinylated PD-1/PDCD1, Human Recombinant	50 μg
6125-50	Acetyl Lysine (Biotin) Antibody	50 μg
K109-100	Annexin V-Biotin Apoptosis Kit	100 assays
K109-25	Annexin V-Biotin Apoptosis Kit	25 assays
K109-400	Annexin V-Biotin Apoptosis Kit	400 assays
1003-100	Annexin V-Biotin Reagent	100 µg
1003-1000	Annexin V-Biotin Reagent	1 mg
A1501-50	Anti-human CD9 biotin conjugated antibody	50 µg
7936-1	Core Streptavidin	1 mg
7936-5	Core Streptavidin	5 mg
7936-10	Core Streptavidin	10 mg
9587-5G	D-(+)-Biotin	5 g
9587-10G	D-(+)-Biotin	10 g