8-OHdG ELISA試劑盒 | 貨號K4160-100

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8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA試劑盒 | 貨號K4160-100
8OHdG; 7,8-Dihydro-8-Oxo-2′-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2′-Deoxyguanosine; 8-Oxo-DG
產品規格: 8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA酶聯免疫吸附測定試劑盒 | 提供簡易方便省時之方法,快速測量樣品中8-OHdG 之含量。測量範圍為1.563 – 100 ng/ml。最低靈敏度Sensitivity: < 0.94 ng/ml。BioVision提供一系列準確、即用型之ELISA試劑盒;用於檢測和量化蛋白質或小分子的存在。以ELISA為基礎之偵測方法優點為準確-具有較低的內和測定間變異性的可重複結果、高靈敏度和特異性、低背景;通用性-廣泛的樣品類型,如血清、血漿、組織、細胞培養上清液、尿液和許多其他生物液體。方便性-無需特殊處理或處置。

產品介紹: 8-羥基-2-脫氧鳥苷(8-hydroxy-2-deoxyguanosine , 8-OHdG)是脫氧鳥苷(deoxyguanosine)的氧化衍生物 (oxidized derivative )。 細胞內8-ox-dG (8-hydroxy-2′-deoxyguanosine) 的濃度是oxidative stress的測量指標。 它被認為是oxidative stress和carcinogenesis的關鍵性生物標誌物。 該ELISA試劑盒使用競爭ELISA(Competitive-ELISA)方法。該試劑盒中提供的microtiter plate為8-OHdG預包被。在反應期間,樣品或標準品中的8-OHdG與固定量的8-OHdG在固相支持物上,競爭8-OHdG特異性的生物素化檢測抗體之位點。 樣品中8-OHdG的濃度,通過比較樣品中的O.D.的樣品到標準曲線。8-hydroxy-2′-deoxyguanosine (8-OHdG) is an oxidized derivative of deoxyguanosine. Concentrations of 8-oxo-dG within a cell are a measurement of oxidative stress. It is considered as a critical biomarker of oxidative stress and carcinogenesis. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. During the reaction,8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 8-OHdG. The concentration of 8-OHdG in the samples is determined by comparing the O.D. of the samples to the standard curve.
8-羥基-2-脫氧鳥苷套組內容
Microplate: 96 wells
Lyophilized Standard
Sample / Standard dilution buffer
Biotin- detection antibody (Concentrated)
Antibody dilution buffer
HRP-Streptavidin Conjugate (SABC)
SABC dilution buffer
TMB substrate
Stop Solution
Wash buffer (25X)
Plate sealers
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