Yeast extract 酵母萃取物 (yeast extract) |高 E. Coli DH5α 密度 LABM貨號MC001-A (NCM0218A)
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Yeast extract | 酵母萃取物(yeast extract) |貨號MC001-A (NCM0218A)
可以提高 E. Coli DH5α 密度(V.S. 傳統 LB broth )。Yeast extract 提供了豐富的營養成分，Saccharomyces酵母細胞經過自動分解(autolysate)後，除了富含各式微生物以及動物細胞生長所需的胺基酸氮源外，還有含量極高的可溶性維生素B群 (B vitamins)。包括 vitamins、amino acids、peptides 與 carbohydrates 等。其高營養價值也是胎牛血清 FBS 最佳替代物，是生物製程中極具重要性的非動物性蛋白之來源。因此培養基配方、微生物發酵原料、血清替代物、抗體生產與蛋白質藥物等都採用yeast extract作為營養來源。
Yeast Extract is an autolysate of yeast cells used in preparing microbiological culture media in a laboratory setting. Yeast Extract is not intended for use in the diagnosis of disease or other conditions in humans. Yeast Extract is the water-soluble portion of autolyzed yeast. The autolysis is carefully controlled to preserve naturally occurring B-complex vitamins. Yeast Extract is prepared and standardized for bacteriological use and cell cultures and is an excellent stimulator of bacterial growth. Yeast Extract is generally employed in the concentration of 0.3% – 0.5%. Yeast Extract is typically prepared by growing baker’s yeast, Saccharomyces spp., in a carbohydrate-rich plant medium. The yeast is harvested, washed, and resuspended in water, where it undergoes autolysis, i.e., self-digestion using the yeast’s enzymes. Yeast Extract is the total soluble portion of this autolytic action. The autolytic activity is stopped by a heating step. The resulting Yeast Extract is filtered clear and dried into a powder by spray drying. Yeast Extract has been successful in culture media for bacterial studies in milk and other dairy products. Several media containing Yeast Extract have been recommended for cell culture applications.
| Specifications | ||
|---|---|---|
| Brand | Neogen® Culture Media | |
| Analyte | Non-selective | |
| Platform | Dehydrated Culture Media | |
| Mass | 500 g | |
| Previous Item Number | Product was previously known as Acumedia Product: 7184 Yeast Extract; and LabM Product: MC001 Yeast Extract Powder | |
| Package Weight | 1.35 | |
LAB M 粉末培養基 | 英國原裝進口
LAB M位於英國，是培養基老品牌公司，口碑好品質佳，採用密封封口膜，原裝進口培養基不易受潮，亦沒有分裝預混之疑慮。Harlequin™呈色培養基系列商品與各式培養基添加物，提供快速和準確的方法分離與判讀目標微生物。雙色判讀，簡易快速；在食品安全及臨床診斷皆符合相關規範（ISO 9001:2000和ISO13485，以及歐洲IVD指令CE標誌）。
分生專用培養基LB Broth與LB Agar，方便進行DNA Cloning、Yeast培養等實驗，是 E. Coli 表現系統最佳的選擇！
Tryptone | 酪蛋白(casein)水解物 | 貨號MC005-A
Tryptone常見於微生物培養與發酵使用，也是美國藥典規範的標準培養基成分之ㄧ。Tryptone是酪蛋白(casein)經由胰臟酵素水解(pancreatic digest)而成的營養成分，也是一般所謂胰水解蛋白的通用名稱。酪蛋白是牛奶中主要的蛋白質成分，富含各種胺基酸氮源，尤其是色胺酸(tryptophan)比例相當高。Tryptone的用途很廣，是LB medium、Terrific broth、Tryptic soy medium (TSB)與Fluid Thioglycollate Medium (FTM)等常用培養基的主要成分，也是抗生素、胞外蛋白質(extra-cellular protein)、干擾素(interferon)與疫苗毒素等發酵製程中重要的培養原料。
Plate count agar，PCA培養皿計數培養基\總生菌數培養基 | 貨號LAB149-A
國際標準PCA，用於總細菌中的測定（GB2008、SN標準）。商品化細菌用培養基PCA：主要用於細菌培養；或亦可選取胰蛋白大豆瓊脂培養基（Tryptone soy agar，TSA）二者之一培養細菌。
黴菌及酵母菌培養，使用沙氏葡萄糖培養基（Sabouraud Dextrose Agar，SDA） 或馬鈴薯葡萄糖培養基（Potato dextrose agar，PDA）。Potato Dextrose Agar | 馬鈴薯葡萄糖洋菜培養基 |貨號LAB098-A
| 產品編號 | 產品名稱 | 包裝 |
| LAB004-A | Tryptone Soy Broth USP (Soybean Casein Digest Medium) | 500g |
| LAB011-A | Tryptone Soy Agar USP (Soybean Casein Digest Medium) | 500g |
| LAB028-A | Blood Agar Base | 500g |
| LAB061-A | Eosin Methylene Blue Agar (Levine) | 500g |
| LAB093-A | MRS Agar | 500g |
| LAB094-A | MRS Broth | 500g |
| LAB098-A | Potato Dextrose Agar | 500g |
| LAB108-A | Pseudomonas Agar Base | 500g |
| LAB149-A | Plate Count Agar | 500g |
| LAB168-A | L.B. Agar | 500g |
| LAB169-A | LB Broth | 500g |
| LAB196-A | Lauryl Tryptose Broth | 500g |
| MC001-A | Yeast Extract Powder | 500g |
| MC003-A | Soy Peptone | 500g |
| MC005-A | Tryptone | 500g |
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