5-HT(5-羥色胺) ELISA 試劑盒 | ELK Biotechnology貨號ELK7830

5-HT (5-Hydroxytryptamine, 5-HT) Competitive Inhibition ELISA Kit 

靈敏度14.07-900 ng/mL、最低靈敏度3.94 ng/mL

產品介紹:

5-羥色胺(5-Hydroxytryptamine, 5-HT) 試劑盒 ELISA試劑盒 | 貨號ELK8629 (Competitive Inhibition ELISA)，偵測的靈敏度14.07-900 ng/mL、最低靈敏度3.94 ng/mL。本檢測採用競爭性抑制酵素免疫測定技術。試劑盒提供的微孔板已預先包覆5-羥色胺（(5-Hydroxytryptamine, 5-HT) 。將標準品或樣本加入對應的微孔板孔中，然後加入生物素標記的5-羥色胺 (5-Hydroxytryptamine, 5-HT) 特異性抗體。接下來，在每個微孔板孔中加入辣根過氧化物酶（HRP）標記的親和素並進行孵育。之後加入TMB底物溶液。加入硫酸溶液終止酵素-底物反應，並在450 nm ± 10 nm波長下以分光光度計測量顏色變化。透過比較樣品的吸光度值與標準曲線，確定樣品中5-羥色胺(5-Hydroxytryptamine, 5-HT) 的濃度。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA。可應用於serum, plasma, tissue homogenates, cell lysates, cell culture。並在 450nm ± 10nm 的波長下用分光光度計測量顏色變化。然後通過將樣品的 OD 與標準曲線進行比較來確定樣品中5-羥色胺(5-Hydroxytryptamine, 5-HT) ELISA試劑盒的濃度。

 

Product name:	5-HT(5-Hydroxytryptamine) ELISA Kit
Reactivity:	General
Alternative Names:	5HT; Serotonin; N-Methyl-Gamma
Assay Type:	Competitive Inhibition
Sensitivity:	3.94 ng/mL
Standard:	900 ng/mL
Detection Range:	14.07-900 ng/mL
Sample Type:	serum, plasma
Assay Length:	2h
Research Area:	Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

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ELK Biotechnology公司是一家專注於生命科學、免疫研究的高科技生物企業，位於Biolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology是一家專注於免疫產品和分子生物學產品研發和生產的高科技生物公司。產品涵蓋ELISA試劑盒、抗體、基因組提取試劑、分子生物學試劑。涉及免疫化學、腫瘤研究、神經生物學、細胞週期、信號轉導等領域。我們的產品有嚴格的研發和質量檢測流程。每個產品都有完整的研發、生產和質檢記錄。經驗豐富的研發人員，完善的質檢支持部門，嚴格的檢測流程，為客戶提供質量可靠的科研產品。