細胞代謝檢測試劑盒 (Cell metabolism detection kit)- γ-氨基丁酸 (GABA) 含量檢測

γ-氨基丁酸 (GABA) 含量檢測試劑盒 | γ-Aminobutyric Acid (GABA) Content Detection Kit Abbkine貨號KTB3045

CheKine™ Micro γ-Aminobutyric Acid (GABA) Content Detection Kit

產品介紹:

細胞代謝 (Cell metabolism) 是指細胞內為維持生命而發生的一系列有序化學反應的總稱。其產生的產物參與細胞凋亡 (cell apoptosis)、訊號傳導 (signaling)、免疫反應(immune response)、代謝調控(metabolic regulation)、基因表現(gene expression)等過程，與老化 (aging)、代謝功能障礙 (metabolic dysfunction)、代謝失調 (metabolic disorders) 等疾病密切相關。細胞代謝活性(cell metabolic activity) 的測定可作為細胞研究的重要指標。Abbkine CheKine™ 細胞代謝產品線包含大量相關的代謝物(metabolites)、酵素(enzymes)、營養素(nutrients)和代謝物(metabolites )定量檢測試劑盒等。特色與優勢:  靈敏度高，基於優化的比色法和螢光法原理，無放射性，安全穩定；適用於多種樣品，包括動植物組織、細胞、血清、血漿、細菌、真菌等；實驗過程簡便易行，提供詳細的優化實驗步驟和結果計算方法；實驗結果準確可靠，提供標準物質和標準曲線以供參考。操作便捷，靈敏度高，不受其他物質干擾，準確度和特異性更高。

γ-氨基丁酸（γ-Aminobutyric Acid, GABA）是一種非蛋白源性胺基酸(non-proteinogenic amino acid)，由四個碳原子組成(four carbon atoms)，廣泛存在於植物(plants)、動物(animals)和微生物(microorganisms)中。在植物體內，γ-氨基丁酸（γ-Aminobutyric Acid, GABA）主要由麩胺酸脫羧酶催化L-麩胺酸脫羧生成。在哺乳動物大腦中，γ-氨基丁酸（γ-Aminobutyric Acid, GABA）是一種有效的抑制性神經傳導物質，具有降低血壓(blood pressure)、增強腦活力(enhancing brain vitality)、滋養神經細胞(nourishing nerve cells)、維持神經穩定性(neural stability)、促進生長激素分泌(growth hormone secretion)以及有益於肝腎健康等功能。它已在醫藥和保健食品領域中廣泛應用。測定原理：GABA與苯酚(phenol)和次氯酸鈉(sodium hypochlorite)反應生成藍綠色產物，該產物在640 nm處具有最大吸收值。

Product name	CheKine™ Micro γ-Aminobutyric Acid (GABA) Content Detection Kit
SampleType	Animal or plant tissues
Alternative	γ-Aminobutyric Acid (GABA)
Kit components	•Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ
Features & Benefits	The verified samples are complete in type and easy to operate．
Usage notes	• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If  don’t perform the assay at the same time, it’s better to complete the Sample Preparation step before storing the samples.
Storage instructions	Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	γ-Aminobutyric Acid (GABA) is a non-proteinogenic amino acid consisting of four carbon atoms, which is widely present in the organisms of plants, animals, and microorganisms. Within plants, GABA is primarily generated through decarboxylation of L-glutamate catalyzed by glutamate decarboxylase. In the mammalian brain, GABA serves as an efficacious inhibitory neurotransmitter, exhibiting functions such as lowering blood pressure, enhancing brain vitality, nourishing nerve cells, maintaining neural stability, promoting growth hormone secretion, and benefiting liver and kidney health. It has already found extensive applications in both pharmaceuticals and health food products.Principle of Determination: GABA reacts with phenol and sodium hypochlorite, giving rise to a blue-green product that exhibits a maximum absorbance at 640 nm.
Alternative	γ-Aminobutyric Acid (GABA)

樣品製備: 

注意：建議使用新鮮樣品。如不立即進行檢測，樣本可在-80℃下保存一個月。檢測時，應控制解凍的溫度和時間。室溫解凍時，樣品應在4小時內解凍。稱取0.1 g組織樣品，加入1 mL萃取緩衝液，充分混勻，然後將混合物轉移至EP管中。在95℃水浴中孵育2小時（確保管蓋緊閉，並在管口周圍貼上密封膜以防止水分蒸發）。冷卻後，在25℃下以8,000 g離心10分鐘。收集上清液進行後續分析。