CD274體內應用抗體 (In Vivo antibody) | Clone 10F.9G2 InvivoCrown貨號 IV0101
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InVivoPro系列單株抗體 (體內抗體) ->>CD274 (B7-H1和PD-L1)
InVivoPro Anti-Mouse PD-L1(B7-H1, CD274) in vivo antibody, Clone 10F.9G2
CD274，又稱為B7-H1和PD-L1，是一種分子量為40 kD的I型跨膜蛋白，屬於免疫球蛋白受體超家族(immunoglobulin receptor superfamily)中的B7家族成員(B7 family)。它表達於T細胞、B細胞、NK細胞、樹突狀細胞(dendritic cells)、IFN-γ活化的內皮細胞和單核細胞(monocytes)。內毒素≤ 1.0 EU/mg, by the LAL method，應用於In vivo PD-L1 blocked。InVivoPro 抗小鼠 PD-1(CD279) 體內抗體（clone 29F.1A12）已成為臨床前免疫治療研究(preclinical immunotherapy research)的基石。這款高度特異的單株抗體由 BioXCell 開發，可針對小鼠 PD-1。 PD-1 是一種關鍵的免疫檢查點分子(immune checkpoint molecule)，可抑制 T 細胞反應(T-cell responses)，使腫瘤( tumors)和病原體(pathogens)逃避免疫檢測(immune detection)。克隆29F.1A12源自大鼠IgG2a克隆(rat IgG2a clone)，在嚴格的animal-free conditions下生產，確保卓越的純度（≥95%）和超低內毒素水平（<1 EU/mg）。它以高親和力和特異性與PD-1胞外結構域結合，有效阻斷其與PD-L1和PD-L2配體的相互作用。透過阻斷此抑制性訊號通路，此抗體可重振T細胞功能，增強其對癌細胞的細胞毒性能力(cytotoxic ability)，並增強抗病毒免疫力( antiviral immunity)。
在臨床前腫瘤學(oncology)研究中，Clone 29F.1A12已顯示出顯著的療效。在小鼠黑色素瘤(melanoma)、大腸直腸癌(colorectal cancer)和乳癌(breast cancer)模型中，此抗體治療可顯著抑制腫瘤生長(tumor growth suppression)。它促進 CD8+ T 細胞的活化(activation)和增殖(proliferation)，同時降低腫瘤微(tumor microenvironment)環境中耗竭 T 細胞的比例。Clone 29F.1A12與其他免疫療法藥物（如抗 CTLA-4 抗體或免疫刺激性細胞激素）合併使用時，可發揮協同作用，進一步增強抗腫瘤(antitumor)反應。除了癌症研究(cancer research)之外，這種抗體在傳染病研究( infectious disease studies)中也具有不可估量的價值。在包括鼠鉅細胞病毒(viral infections)和淋巴球脈絡叢腦膜炎病毒(lymphocytic choriomeningitis virus)在內的病毒感染模型中，Clone 29F.1A12可恢復抗病毒T細胞活性，促進病毒清除並預防慢性感染的發展。它還有助於闡明PD-1在持續性病毒感染(viral infections)期間免疫衰竭中的作用。Clone 29F.1A12 的性能已通過多種實驗方法得到嚴格驗證。流式細胞儀( Flow cytometry )證實其與PD-1+ T細胞特異性結合，而免疫組織化學染色(immunohistochemistry)則可觀察到組織中PD-1的表達。藥物動力學研究已確定最佳給藥方案，通常為 intraperitoneal administration 給藥，劑量為2.5-7.5 mg/kg，每週兩次。
CD274體內應用抗體(In Vivo antibody) | Clone: 10F.9G2 InvivoCrown貨號 IV0101
產品介紹
InVivoPro致力於為科學家提供純度高、適合研究領域的體內應用抗體(In Vivo antibody)，不斷擴展的產品組合包含 20 多種高品質單株抗體 (MAb)，每種都經過嚴格的體內應用( in vivo applications)驗證。這些經過科學表徵的試劑，同時可立即提供毫克至數克的量級，並由英國的快速配送提供支持，以確保可靠的供應鏈連續性。主要產品包括：針對特定表位( epitopes )的阻斷性 MAb、用於機制藥理學研究的功能性 MAb (Functional MAbs) 和生物仿製藥對照(biosimilar controls)、 針對物種有特異性的抗鼠試劑(anti-mouse reagents)，經過優化以消除交叉反應混雜(cross-reactivity confounders)因素。所有產品均經過三級品質保證(three-tier quality assurance)，確保在臨床前模型(preclinical models.)中的可重複性。
| Catalog | IV0101 |
| Product Name | InVivoPro Anti-Mouse PD-L1(B7-H1, CD274) in vivo antibody, Clone 10F.9G2 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG2b κ |
| Clone | 10F.9G2 |
| Target | PD-L1 |
| Other Names | Programmed cell death ligand 1，B7-H1，CD274，PD-L1 |
| Isotype Control Catalog Code | IV0106 or Rat IgG2b Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 200-500 μg per mouse; or 10 mg/kg. This range is based off the most recent publication data using the 10F.9G2 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background(ichorbio) | CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. |
| Applications | In vivo PD-L1 blocked |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
InvivoCrown
https://www.crownzz.com/
| InvivoCrown 產品貨號 | InvivoCrown 品項 | 網站資料 | 操作手冊 |
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