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白蛋白(Alb)含量測定試劑盒 | CheKine™ Micro Albumin (Alb) Content Assay Kit Abbkine貨號KTB2221

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白蛋白(Alb)含量測定試劑盒操作手冊

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白蛋白(Alb)含量測定試劑盒 | CheKine™ Micro Albumin (Alb) Content Assay Kit Abbkine貨號KTB2221

CheKine™ Micro Albumin (Alb) Content Assay Kit

產品介紹:

Albumin is synthesized by hepatocytes and is the most abundant protein in plasma. It plays important physiological roles,including the maintenance of colloid osmotic pressure, and the binding of long-chain fatty acids, bile acids, bilirubin, heme, calcium, and magnesium ions. Albumin possesses antioxidant and anticoagulant properties, acts as a carrier for nutrients and drugs, and also functions as a buffer for plasma pH. The level of serum albumin is directly associated with the progression of liver diseases, kidney diseases, malnutrition, or protein-losing enteropathies, making it an important indicator in clinical diagnostics. CheKine™ Micro Albumin (Alb) Content Assay Kit provides a simple, convenient, and rapid method for determining albumin content, suitable for both serum and urine samples. The principle is based on the fact that albumin carries a positive charge in a buffer solution at pH 4.2, and can bind to the negatively charged dye bromocresol green to form a blue-green complex. This complex has an absorption peak at 630 nm, and within a certain range, the intensity of the color is directly proportional to the concentration of albumin.

白蛋白(Albumin)由肝細胞(hepatocytes)合成,是血漿中最豐富的蛋白質。它發揮著重要的生理作用,包括維持膠體滲透壓( osmotic pressure),以及與長鏈脂肪酸(long-chain fatty acids)、膽汁酸(bile acids)、膽紅素( bilirubin)、血紅素(heme)、鈣(calcium)和鎂離子(magnesium ions)的結合。白蛋白(Albumin )具有抗氧化(antioxidant)和抗凝血(anticoagulant)特性,可作為營養物質和藥物的載體,並作為血漿pH值的緩衝劑。血清白蛋白水平與肝病(liver diseases)、腎病(kidney diseases)、營養不良或蛋白質丟失性腸病的進展直接相關,是臨床診斷的重要指標。 CheKine™ 微量白蛋白(Alb)含量測定試劑盒提供了一種簡單、方便、快速的測定白蛋白含量的方法,適用於血清和尿液樣本。其原理是基於白蛋白在pH 4.2的緩衝溶液中帶正電荷,可以與帶負電荷的染料溴甲酚綠結合形成藍綠色複合物。此複合物在630nm處有吸收峰,在一定範圍內,顏色的強度與白蛋白的濃度成正比。

Product name CheKine™ Micro Albumin (Alb) Content Assay Kit
SampleType Serum, Plasma, and Urine samples
Alternative Albumin (Alb)
Kit components • ReagentⅠ
•Standard
Features & Benefits The verified samples are complete in type and easy to operate.
Usage notes • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don’t perform the assay at the same time, it’s better to complete the Sample Preparation step before storing the samples.
Storage instructions Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
background Albumin is synthesized by hepatocytes and is the most abundant protein in plasma. It plays important physiological roles,including the maintenance of colloid osmotic pressure, and the binding of long-chain fatty acids, bile acids, bilirubin, heme, calcium, and magnesium ions. Albumin possesses antioxidant and anticoagulant properties, acts as a carrier for nutrients and drugs, and also functions as a buffer for plasma pH. The level of serum albumin is directly associated with the progression of liver diseases, kidney diseases, malnutrition, or protein-losing enteropathies, making it an important indicator in clinical diagnostics. CheKine™ Micro Albumin (Alb) Content Assay Kit provides a simple, convenient, and rapid method for determining albumin content, suitable for both serum and urine samples. The principle is based on the fact that albumin carries a positive charge in a buffer solution at pH 4.2, and can bind to the negatively charged dye bromocresol green to form a blue-green complex. This complex has an absorption peak at 630 nm, and within a certain range, the intensity of the color is directly proportional to the concentration of albumin.
Alternative Albumin (Alb)

樣品製備: 

樣品製備
注意:建議使用新鮮樣品。如不立即檢測,樣本可在-80℃保存,最多可保存一個月。樣品製備過程中應控制解凍溫度和時間。室溫解凍應在4小時內完成。 1. 血清、血漿樣本:可直接測定。 2. 尿液樣品:可直接測定。

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